Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,951,838 G→T 11.5% P568H (CCT→CAT) ‡ mnmC ← methyltransferase/FAD‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,951,8380GT11.5% 176.4 / 10.9 59P568H (CCT→CAT) ‡mnmCmethyltransferase/FAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (24/28);  new base T (4/3);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCAGCAGCGCCAGGTTGCCCTGGTGCTC  >  NC_002947/1951690‑1951983
                                                                                                                                                    |                                                                                                                                                 
aCTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAg                                                                                                                                                   >  8:239044/1‑149 (MQ=255)
 cTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAgg                                                                                                                                                  >  7:251042/1‑149 (MQ=255)
  tCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAggg                                                                                                                                                 >  1:6338/1‑149 (MQ=255)
  tCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAggg                                                                                                                                                 <  4:1685/149‑1 (MQ=255)
  tCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAggg                                                                                                                                                 >  2:311829/1‑149 (MQ=255)
    aaCGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGcc                                                                                                                                               <  1:263908/149‑1 (MQ=255)
    aaCGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGc                                                                                                                                                <  1:311829/148‑1 (MQ=255)
      ctgTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCcga                                                                                                                                             <  1:229090/147‑1 (MQ=255)
       ggTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCcgac                                                                                                                                            <  2:229193/149‑1 (MQ=255)
       ggTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCCCTATCGCcgac                                                                                                                                            <  5:112777/149‑1 (MQ=255)
           ccGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATc                                                                                                                                        <  7:239044/149‑1 (MQ=255)
                  cAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAgg                                                                                                                                  >  4:119019/1‑148 (MQ=255)
                   aaCCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCcga                                                                                                                                             >  3:309340/1‑136 (MQ=255)
                   aaCCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCcga                                                                                                                                             <  4:309340/136‑1 (MQ=255)
                   aaCCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCCCTATCGCcgacgc                                                                                                                                          >  3:19408/1‑138 (MQ=255)
                   aaCCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCCCTATCGCcgacgc                                                                                                                                          <  4:19408/139‑2 (MQ=255)
                       cgcgTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAg                                                                                                                                   >  2:322732/1‑142 (MQ=255)
                       cgcgTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAg                                                                                                                                   <  1:322732/142‑1 (MQ=255)
                          gTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCggg                                                                                                                         <  7:252799/149‑1 (MQ=255)
                             aGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTg                                                                                                                      <  3:119019/149‑1 (MQ=255)
                              gCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTg                                                                                                                      <  2:217573/148‑1 (MQ=255)
                              gCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAAAGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTg                                                                                                                      >  1:217573/1‑148 (MQ=255)
                               ccATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCCCTATCGCcgac                                                                                                                                            <  3:105245/125‑1 (MQ=255)
                               ccATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCCCTATCGCcgac                                                                                                                                            >  4:105245/1‑125 (MQ=255)
                                   gCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCaa                                                                                                                >  8:210110/1‑149 (MQ=255)
                                       gCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACgg                                                                                                             >  3:155525/1‑148 (MQ=255)
                                         tGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAAc                                                                                                          >  6:1681/1‑149 (MQ=255)
                                           ttCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACgc                                                                                                        <  1:311126/149‑1 (MQ=255)
                                             cacaTACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACgca                                                                                                       <  5:1681/148‑1 (MQ=255)
                                              acaTACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACgcagc                                                                                                     <  8:251042/149‑1 (MQ=255)
                                              acaTACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACgcagc                                                                                                     <  7:210110/149‑1 (MQ=255)
                                                aTACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAg                                                                                                                                   >  1:266762/1‑117 (MQ=255)
                                                aTACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAg                                                                                                                                   <  2:266762/117‑1 (MQ=255)
                                                 tACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACg                                                                                                  >  1:134535/1‑149 (MQ=255)
                                                   cAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTc                                                                                                >  7:107048/1‑149 (MQ=255)
                                                    aaCCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTcc                                                                                               >  7:46132/1‑149 (MQ=255)
                                                       ccGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTg                                                                                            <  2:134535/149‑1 (MQ=255)
                                                           ccAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAAt                                                                                        >  1:58698/1‑149 (MQ=255)
                                                                cAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTc                                                                                   >  6:206443/1‑149 (MQ=255)
                                                                    ggcagggcaCATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCgggg                                                                               <  3:249897/149‑1 (MQ=255)
                                                                    ggcagggcaCATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCgggg                                                                               >  4:249897/1‑149 (MQ=255)
                                                                           cacaTCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGtt                                                                        <  5:206443/149‑1 (MQ=255)
                                                                              aTCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTc                                                                                   <  3:231855/135‑1 (MQ=255)
                                                                              aTCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTc                                                                                   >  4:231855/1‑135 (MQ=255)
                                                                               tCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTcagc                                                                    <  6:25253/149‑1 (MQ=255)
                                                                                            gcgcATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCggg                                                                                >  5:67494/1‑124 (MQ=255)
                                                                                            gcgcATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCggg                                                                                <  6:67494/124‑1 (MQ=255)
                                                                                                  cccGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTgcgc                                                  >  6:261635/1‑148 (MQ=255)
                                                                                                          aGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGAc                                         <  8:107048/149‑1 (MQ=255)
                                                                                                                 cATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAAtt                                  >  7:73528/1‑149 (MQ=255)
                                                                                                                 cATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAAtt                                  >  7:213166/1‑149 (MQ=255)
                                                                                                                     ggCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCg                               <  2:6338/148‑1 (MQ=255)
                                                                                                                      gCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTc                             >  3:127099/1‑149 (MQ=255)
                                                                                                                            gCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCg                                                                                  <  6:76960/90‑1 (MQ=255)
                                                                                                                            gCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCg                                                                                  >  5:76960/1‑90 (MQ=255)
                                                                                                                             cAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCagcagc                      <  8:46132/149‑1 (MQ=255)
                                                                                                                                  gCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCAGCAGCGCCAg                 <  2:58698/149‑1 (MQ=255)
                                                                                                                                    cTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCAGCAGCGCCAGGt               >  5:339982/1‑149 (MQ=255)
                                                                                                                                    cTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCAGCAGCGCCAGGt               >  3:45942/1‑149 (MQ=255)
                                                                                                                                      gatcgTCGGCCCTATCGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCAGCAGCGCCAGGTTg             >  4:292532/4‑149 (MQ=255)
                                                                                                                                       atcgTCGGCCCTATCGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCAGCAGCGCCAGGTTGc            >  5:177977/3‑149 (MQ=255)
                                                                                                                                           tCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCAGCAGCGCCAGGTTGCCCTg        >  3:15152/1‑149 (MQ=255)
                                                                                                                                             ggCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCAGCAGCGGCAGGTTGCCCTGGt      <  4:15152/149‑1 (MQ=255)
                                                                                                                                                 aTAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCAGCAGCGCCAGGTTGCCCTGGTGCTc  <  4:45942/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCATCCCGGCCCAGTACTGCATAGGCTTCGGCAAACGCCTGCGCGTCGGCGATAGGGCCGACGATCGGCAGGTAATCCGGGCTGGTGCAACGGAACGCAGCACGTCCCTGCAATTGCTCGGGGTCCAGTTCAGCCACAGCCAGCCGTTGCGCCAGATCGACGGAAATTTCGTCCAGCAGCGCCAGGTTGCCCTGGTGCTC  >  NC_002947/1951690‑1951983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑