Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,072,914 G→A 9.3% T165I (ACC→ATC)  PP_1847 ← TonB‑dependent ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,072,9140GA 9.3% 118.3 / 2.4 43T165I (ACC→ATC) PP_1847TonB‑dependent ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (22/17);  new base A (2/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

TTCATGGGCCAGGTTGGCGCGCAGGCCGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGCTACTGGCGG  >  NC_002947/2072781‑2073061
                                                                                                                                     |                                                                                                                                                       
ttCATGGGCCAGGTTGGCGCGCAGGCCGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATc                                                                                                                                           >  7:21686/1‑148 (MQ=255)
               ggCGCGCAGGCCGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTcc                                                                                                                            <  7:173253/148‑1 (MQ=255)
                         ccGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGc                                                                                                                     <  6:156299/145‑1 (MQ=255)
                         ccGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGc                                                                                                                     >  5:156299/1‑145 (MQ=255)
                          cGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGATCAACTATGTGACCAAgcg                                                                                                                                      <  4:2703/127‑2 (MQ=255)
                          cGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGATCAACTATGTGACCAAgcg                                                                                                                                      >  3:2703/1‑126 (MQ=255)
                           gAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCt                                                                                                               <  8:21686/149‑1 (MQ=255)
                                 cTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGcc                                                                                                      >  2:108698/1‑149 (MQ=255)
                                 cTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGcc                                                                                                      >  2:228159/1‑149 (MQ=255)
                                       gCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGa                                                                                                  >  4:74796/1‑147 (MQ=255)
                                       gCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGAGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGa                                                                                                  >  8:36418/1‑147 (MQ=255)
                                        cTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAg                                                                                               >  1:268060/1‑149 (MQ=255)
                                         tGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCaa                                                                                                                                         <  4:234948/109‑1 (MQ=255)
                                         tGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCaa                                                                                                                                         >  3:234948/1‑109 (MQ=255)
                                         tGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAgg                                                                                              >  4:178543/1‑149 (MQ=255)
                                             gAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCG‑GCTTGATCAACTATGTGACCAAgcgcgc                                                                                                                                   >  3:329475/1‑106 (MQ=37)
                                             gAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCG‑GCTTGATCAACTATGTGACCAAgcgcgc                                                                                                                                   <  4:329475/110‑5 (MQ=37)
                                                 cAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAg                                                                                      <  2:268060/149‑1 (MQ=255)
                                                  aGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAg                                                                                      >  4:9775/1‑148 (MQ=255)
                                                     cgccAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCgg                                                                                                                  >  6:253749/1‑120 (MQ=255)
                                                     cgccAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGccc                                                                                                     >  2:215048/1‑130 (MQ=255)
                                                     cgccAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGccc                                                                                                     <  1:215048/130‑1 (MQ=255)
                                                        cAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAg                                                                                      <  5:168080/142‑1 (MQ=255)
                                                        cAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAg                                                                                      >  6:168080/1‑142 (MQ=255)
                                                              cGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACctgc                                                                         <  3:9775/149‑1 (MQ=255)
                                                                 tGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCtgctgc                                                                      <  3:178543/149‑1 (MQ=255)
                                                                        gTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGttt‑t                                                              >  1:330566/1‑149 (MQ=255)
                                                                        gTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGttt                                                                >  4:285034/1‑148 (MQ=255)
                                                                           gCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGTCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCa                                                           <  3:285034/149‑1 (MQ=255)
                                                                              ttCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTAcc                                                                            >  4:264896/1‑130 (MQ=255)
                                                                              ttCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTAcc                                                                            <  3:264896/130‑1 (MQ=255)
                                                                                              gTCGATACCGTGACGCTGCGTATGTCTTCGGGGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTcc                                        >  3:163933/1‑149 (MQ=255)
                                                                                               tCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCg                                       <  3:74796/149‑1 (MQ=255)
                                                                                                 gATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAg                                                                                                        >  2:285898/1‑83 (MQ=255)
                                                                                                       gTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCAAATAGTTTATCAAGCCGCCCGGCTCCTACACGCCGGTCTGCCTCCGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAAt                                  <  1:108698/149‑1 (MQ=255)
                                                                                                          aCGCTGCGTATGTCTTCGGGGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCt                             <  4:163933/148‑1 (MQ=255)
                                                                                                           cGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTTCTCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTg                            <  1:228159/149‑1 (MQ=255)
                                                                                                              tGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCt                                               >  7:148044/1‑126 (MQ=255)
                                                                                                              tGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCt                                               <  8:148044/126‑1 (MQ=255)
                                                                                                               gCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACatgc                                                                         >  2:177168/1‑100 (MQ=255)
                                                                                                                    tGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATg                  >  3:198390/1‑149 (MQ=255)
                                                                                                                           ggCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGc           <  7:36418/149‑1 (MQ=255)
                                                                                                                           ggCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGc           >  8:32418/1‑149 (MQ=255)
                                                                                                                                    ggTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑CCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGCTACTggcgg  >  4:90334/1‑149 (MQ=255)
                                                                                                                                     |                                                                                                                                                       
TTCATGGGCCAGGTTGGCGCGCAGGCCGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGCCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGCTACTGGCGG  >  NC_002947/2072781‑2073061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑