Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,072,927 T→C 10.4% I161V (ATC→GTC)  PP_1847 ← TonB‑dependent ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,072,9270TC10.4% 123.0 / 6.1 48I161V (ATC→GTC) PP_1847TonB‑dependent ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base T (25/18);  new base C (2/3);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

TTCATGGGCCAGGTTGGCGCGCAGGCCGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGCTACTGGCGGCGTTCAG  >  NC_002947/2072781‑2073068
                                                                                                                                                  |                                                                                                                                                  
ttCATGGGCCAGGTTGGCGCGCAGGCCGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATc                                                                                                                                                   >  7:21686/1‑148 (MQ=255)
               ggCGCGCAGGCCGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTcc                                                                                                                                   <  7:173253/148‑1 (MQ=255)
                         ccGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGc                                                                                                                            <  6:156299/145‑1 (MQ=255)
                         ccGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGc                                                                                                                            >  5:156299/1‑145 (MQ=255)
                          cGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGATCAACTATGTGACCAAgcg                                                                                                                                              <  4:2703/127‑2 (MQ=255)
                          cGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGATCAACTATGTGACCAAgcg                                                                                                                                              >  3:2703/1‑126 (MQ=255)
                           gAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCt                                                                                                                      <  8:21686/149‑1 (MQ=255)
                                 cTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGcc                                                                                                             >  2:108698/1‑149 (MQ=255)
                                 cTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGcc                                                                                                             >  2:228159/1‑149 (MQ=255)
                                       gCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGa                                                                                                         >  4:74796/1‑147 (MQ=255)
                                       gCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGAGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGa                                                                                                         >  8:36418/1‑147 (MQ=255)
                                        cTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAg                                                                                                      >  1:268060/1‑149 (MQ=255)
                                         tGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCaa                                                                                                                                                 <  4:234948/109‑1 (MQ=255)
                                         tGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCaa                                                                                                                                                 >  3:234948/1‑109 (MQ=255)
                                         tGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAgg                                                                                                     >  4:178543/1‑149 (MQ=255)
                                             gAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCG‑GCTTGATCAACTATGTGACCAAgcgcgc                                                                                                                                           >  3:329475/1‑106 (MQ=37)
                                             gAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCG‑GCTTGATCAACTATGTGACCAAgcgcgc                                                                                                                                           <  4:329475/110‑5 (MQ=37)
                                                 cAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAg                                                                                             <  2:268060/149‑1 (MQ=255)
                                                  aGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAg                                                                                             >  4:9775/1‑148 (MQ=255)
                                                     cgccAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCgg                                                                                                                         >  6:253749/1‑120 (MQ=255)
                                                     cgccAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGccc                                                                                                            <  1:215048/130‑1 (MQ=255)
                                                     cgccAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGccc                                                                                                            >  2:215048/1‑130 (MQ=255)
                                                        cAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAg                                                                                             >  6:168080/1‑142 (MQ=255)
                                                        cAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAg                                                                                             <  5:168080/142‑1 (MQ=255)
                                                              cGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACctgc                                                                                <  3:9775/149‑1 (MQ=255)
                                                                 tGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCtgctgc                                                                             <  3:178543/149‑1 (MQ=255)
                                                                        gTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGttt‑t                                                                     >  1:330566/1‑149 (MQ=255)
                                                                        gTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGttt                                                                       >  4:285034/1‑148 (MQ=255)
                                                                           gCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGTCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCa                                                                  <  3:285034/149‑1 (MQ=255)
                                                                              ttCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTAcc                                                                                   >  4:264896/1‑130 (MQ=255)
                                                                              ttCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTAcc                                                                                   <  3:264896/130‑1 (MQ=255)
                                                                                              gTCGATACCGTGACGCTGCGTATGTCTTCGGGGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTcc                                               >  3:163933/1‑149 (MQ=255)
                                                                                               tCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCg                                              <  3:74796/149‑1 (MQ=255)
                                                                                                 gATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAg                                                                                                               >  2:285898/1‑83 (MQ=255)
                                                                                                       gTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCAAATAGTTTATCAAGC‑CGCCCGGCTCCTACACGCCGGTCTGCCTCCGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAAt                                         <  1:108698/149‑1 (MQ=255)
                                                                                                          aCGCTGCGTATGTCTTCGGGGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCt                                    <  4:163933/148‑1 (MQ=255)
                                                                                                           cGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTTCTCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTg                                   <  1:228159/149‑1 (MQ=255)
                                                                                                              tGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCt                                                      >  7:148044/1‑126 (MQ=255)
                                                                                                              tGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCt                                                      <  8:148044/126‑1 (MQ=255)
                                                                                                               gCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACatgc                                                                                >  2:177168/1‑100 (MQ=255)
                                                                                                                    tGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATg                         >  3:198390/1‑149 (MQ=255)
                                                                                                                           ggCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGc                  <  7:36418/149‑1 (MQ=255)
                                                                                                                           ggCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGc                  >  8:32418/1‑149 (MQ=255)
                                                                                                                                    ggTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑CCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGCTACTggcgg         >  4:90334/1‑149 (MQ=255)
                                                                                                                                        acaTAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGCTACTGGCGGCGtt     >  8:268765/1‑149 (MQ=255)
                                                                                                                                             tgtGACCAAGCGCGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGCTACTGGCGGCGTTCAg  <  4:327795/146‑1 (MQ=255)
                                                                                                                                             gTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGCTACTGGCGGCGtt     >  1:81569/1‑144 (MQ=255)
                                                                                                                                             gTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGCTACTGGCGGCGtt     <  2:81569/144‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTCATGGGCCAGGTTGGCGCGCAGGCCGAACTGCTTTTCGCTGCCGAACCAGCCGCCAAGGTCGGTGGCCAGGTAGCGTTCGCCCTGTTCGTTGGTCGATACCGTGACGCTGCGTATGTCTTCGGCGCGCTTGGTCACATAGTTGATCAAGC‑CGCCCGGCTCCGACACGCCGGTCTG‑‑‑CAGCCCGGACAGGCCCTTGAGCAATTCTACCTGCTGCTTGTTT‑TCCAGGGCTACGTTCTGCTCTCCGGCAATGGTCTGGCCATTGATGCGGTAGCTACTGGCGGCGTTCAG  >  NC_002947/2072781‑2073068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑