Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,103,339	T→G	6.5%	intergenic (+138/+30)	PP_t24 → / ← PP_1879	tRNA‑Gly/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,103,339	0	T	G	6.5%	185.9 / 4.6	62	intergenic (+138/+30)	PP_t24/PP_1879	tRNA‑Gly/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/28);  new base G (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCAGCCTCAACA  >  NC_002947/2103195‑2103484                                                                                                                                                 |                                                                                                                                                   cGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCg                                                                                                                                                >  5:205565/1‑149 (MQ=255)  gCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCgg                                                                                                                                               <  8:141892/149‑1 (MQ=255)  gCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCgg                                                                                                                                               <  1:323127/149‑1 (MQ=255)    tCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGt                                                                                                                                             >  7:49733/1‑149 (MQ=255)     ccAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTGTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTg                                                                                                                                            >  1:175675/1‑149 (MQ=255)     ccAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTg                                                                                                                                            >  4:178479/1‑149 (MQ=255)      cAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTgg                                                                                                                                           <  2:104418/149‑1 (MQ=255)            aaGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTATTTCGGGTGGCG‑tttt                                                                                                                                    >  2:175346/1‑149 (MQ=255)               aTTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑ttttttt                                                                                                                                 >  6:65036/1‑149 (MQ=255)               aTTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑ttttttt                                                                                                                                 >  2:143149/1‑149 (MQ=255)                tctACAGACCTCCGTGGAAGGCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑tttttttt                                                                                                                                >  6:317736/3‑149 (MQ=255)                  tACAGCCTTCCACGTAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑ttttttttt                                                                                                                               <  3:166574/148‑1 (MQ=255)                    cAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑tttttttttgca                                                                                                                            >  3:252231/1‑146 (MQ=255)                       ccTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACCCGAAAGAGTGGCG‑ttttt                                                                                                                                   >  6:324961/1‑139 (MQ=255)                       ccTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACCCGAAAGAGTGGCG‑ttttt                                                                                                                                   <  5:324961/139‑1 (MQ=255)                        cTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCg                                                                                                                        >  4:113026/1‑149 (MQ=255)                             aCGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTa                                                                                                                   >  1:40254/1‑149 (MQ=255)                                gAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACgg                                                                                                                <  3:178479/149‑1 (MQ=255)                                   gTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCtt                                                                                                             <  3:113026/149‑1 (MQ=255)                                    tCTGTAGATCCTCGAAAATATCCGCTTGGGCGTCTTTTTTGGTTTCGGTCAAACCCATCGGGCTCCATGGGCATCCCCAAATTTGGCTTCCCTGCAAAAAACGCCACTCTTTCGGGGGGCG‑TTTTTTTTTTGCATCGGGCTACGGCtt                                                                                                             <  2:175675/148‑1 (MQ=255)                                      tGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCg                                                                                                                                                >  1:290654/1‑112 (MQ=255)                                      tGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCg                                                                                                                                                <  2:290654/112‑1 (MQ=255)                                          gATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTc                                                                                                       <  1:143149/148‑1 (MQ=255)                                          gATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTATTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTc                                                                                                       <  1:175346/148‑1 (MQ=255)                                            tCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCa                                                                                                     <  8:49733/148‑1 (MQ=255)                                            tCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAg                                                                                                    >  8:147223/1‑149 (MQ=255)                                               tCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCGTTTTTTTTTTTGCATCGGGCTACGGCTTGTCTTcc                                                                                                      <  2:241309/145‑1 (MQ=255)                                               tCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCGTTTTTTTTTTTGCATCGGGCTACGGCTTGTCTTcc                                                                                                      >  1:241309/1‑145 (MQ=255)                                                      tAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACCCGAAAGAGTGGCG‑ttttt                                                                                                                                   <  3:246346/108‑1 (MQ=38)                                                          cGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCAt                                                                                      <  2:40254/149‑1 (MQ=255)                                                             ttCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCgg                                                                                                                       >  2:249024/1‑113 (MQ=255)                                                             ttCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCgg                                                                                                                       <  1:249024/113‑1 (MQ=255)                                                                ggCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGa                                                                                 >  2:196776/1‑148 (MQ=255)                                                                 gCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGaaa                                                                               >  4:304568/1‑149 (MQ=255)                                                                   gTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAAtt                                                                             <  7:147223/149‑1 (MQ=255)                                                                     cTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATAGGGCTACTGCTTGTGTTCCAGTTGGGCTGCCGCATCGGGAGAttt                                                                            >  7:154958/1‑148 (MQ=255)                                                                       tttttCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑‑TTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCaa                                                                         <  4:252231/148‑1 (MQ=255)                                                                                   gTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCggg                                                                                                                      <  2:22792/92‑1 (MQ=255)                                                                                   gTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCggg                                                                                                                      >  1:22792/1‑92 (MQ=255)                                                                                      aaaGCCATCGGGATCCATGGGCATCACCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTAtc                                                          <  5:65036/149‑1 (MQ=255)                                                                                          ccATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGGATCGGGCTACGGCTTGTCTTCCAGTTTGGCTGCCGCATCGGGAAATTTCAACCAGCGCAGCTTAtcgtc                                                       >  3:262983/1‑148 (MQ=255)                                                                                           cATCGGGATCCATGGGCATCACCACATTTGGCTTCACTGCAAAAAAAGCCACACTTTCGGGTGCCGTTTTTTTTTTTGCATCGGGCAACGCATTGAGAACCAGTTCTGGTGCCGCAACGGGAAATTTAAACCATCTCATCTTAtcctc                                                       >  8:89745/1‑148 (MQ=255)                                                                                            aTCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGC‑AAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTAAGGCTTGTCTTCCAGTTGGGCTGCCGCATGGGGAAATTTCAAACCGCTAAGATTAACGTCGAAc                                                   >  6:193200/1‑149 (MQ=255)                                                                                                gcaTCCATGGGCATCCCCACATTTGGCTTCACTCCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGac                                                <  5:172115/147‑1 (MQ=255)                                                                                                 gATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCCGGAAATTTCAACCAGCTCAGCTTa                                                            >  7:267039/1‑136 (MQ=255)                                                                                                 gATCCATGGGCATCCACACATTTGGCTTCACTTCAAAAAACGCCACTGTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTa                                                            <  8:267039/136‑1 (MQ=255)                                                                                                          gCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGTATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACcagctcag                                                                <  1:100017/123‑1 (MQ=255)                                                                                                          gCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACcagctcag                                                                >  2:100017/1‑123 (MQ=255)                                                                                                             tCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGaca                                   <  5:115490/149‑1 (MQ=255)                                                                                                              ccccACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGacac                                  <  6:205565/149‑1 (MQ=255)                                                                                                               cccACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGacac                                  <  3:304568/148‑1 (MQ=255)                                                                                                               cccACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACAcc                                 >  8:322469/1‑149 (MQ=255)                                                                                                                ccACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACAccc                                >  7:8179/1‑149 (MQ=255)                                                                                                                  acaTTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCg                               >  8:186633/1‑148 (MQ=255)                                                                                                                          cTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATa                      <  7:322469/149‑1 (MQ=255)                                                                                                                                  cAAAAAACGCCACTCTTTCGGTTGGCT‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCTACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATTGTTGAtt               <  5:343746/148‑1 (MQ=255)                                                                                                                                  cAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCAAAGCGCTACGGCTTGACTTCCAGTTGGGAAGCCGCATCGGGAAATTTCAACCAGCTCAGCTTAACGTCCAACGACACTAACACCGACACCCGGAACAGATAGTTGAtt               >  6:343746/1‑148 (MQ=255)                                                                                                                                   aaaaaaCGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTATTCCAGTTGGGCTGCCGAATCGGGAAATTTCAACCAGCTAAGCTTATCGTCGAACGCCAATTCAACCGACACACGGAACTGAAAGTTGATTGc             >  7:72892/1‑149 (MQ=255)                                                                                                                                   aaaaaaCGCCACCCGAAAGAGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGaa                                                    >  2:84585/1‑110 (MQ=38)                                                                                                                                     aaaaCGCCACTCTTTCGGGTTGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTTCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCa           <  5:193200/149‑1 (MQ=255)                                                                                                                                          gCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGa                                                 <  6:245453/106‑1 (MQ=255)                                                                                                                                          gCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGa                                                 >  5:245453/1‑106 (MQ=255)                                                                                                                                              ctctTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCAGCCTCAACa  <  7:186633/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CGCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCAGCCTCAACA  >  NC_002947/2103195‑2103484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑