Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,103,344	G→A	6.8%	intergenic (+143/+25)	PP_t24 → / ← PP_1879	tRNA‑Gly/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,103,344	0	G	A	6.8%	167.5 / 2.2	59	intergenic (+143/+25)	PP_t24/PP_1879	tRNA‑Gly/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/27);  new base A (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

GCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCAGCCTCAACAGTTCGGTT  >  NC_002947/2103196‑2103492                                                                                                                                                     |                                                                                                                                                      gCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCgg                                                                                                                                                       <  8:141892/149‑1 (MQ=255) gCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCgg                                                                                                                                                       <  1:323127/149‑1 (MQ=255)   tCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGt                                                                                                                                                     >  7:49733/1‑149 (MQ=255)    ccAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTGTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTg                                                                                                                                                    >  1:175675/1‑149 (MQ=255)    ccAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTg                                                                                                                                                    >  4:178479/1‑149 (MQ=255)     cAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTgg                                                                                                                                                   <  2:104418/149‑1 (MQ=255)           aaGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTATTTCGGGTGGCG‑tttt                                                                                                                                            >  2:175346/1‑149 (MQ=255)              aTTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑ttttttt                                                                                                                                         >  2:143149/1‑149 (MQ=255)              aTTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑ttttttt                                                                                                                                         >  6:65036/1‑149 (MQ=255)               tctACAGACCTCCGTGGAAGGCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑tttttttt                                                                                                                                        >  6:317736/3‑149 (MQ=255)                 tACAGCCTTCCACGTAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑ttttttttt                                                                                                                                       <  3:166574/148‑1 (MQ=255)                   cAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑tttttttttgca                                                                                                                                    >  3:252231/1‑146 (MQ=255)                      ccTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACCCGAAAGAGTGGCG‑ttttt                                                                                                                                           <  5:324961/139‑1 (MQ=255)                      ccTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACCCGAAAGAGTGGCG‑ttttt                                                                                                                                           >  6:324961/1‑139 (MQ=255)                       cTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCg                                                                                                                                >  4:113026/1‑149 (MQ=255)                            aCGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTa                                                                                                                           >  1:40254/1‑149 (MQ=255)                               gAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACgg                                                                                                                        <  3:178479/149‑1 (MQ=255)                                  gTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCtt                                                                                                                     <  3:113026/149‑1 (MQ=255)                                   tCTGTAGATCCTCGAAAATATCCGCTTGGGCGTCTTTTTTGGTTTCGGTCAAACCCATCGGGCTCCATGGGCATCCCCAAATTTGGCTTCCCTGCAAAAAACGCCACTCTTTCGGGGGGCG‑TTTTTTTTTTGCATCGGGCTACGGCtt                                                                                                                     <  2:175675/148‑1 (MQ=255)                                         gATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTc                                                                                                               <  1:143149/148‑1 (MQ=255)                                         gATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTATTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTc                                                                                                               <  1:175346/148‑1 (MQ=255)                                           tCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCa                                                                                                             <  8:49733/148‑1 (MQ=255)                                           tCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAg                                                                                                            >  8:147223/1‑149 (MQ=255)                                              tCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCGTTTTTTTTTTTGCATCGGGCTACGGCTTGTCTTcc                                                                                                              <  2:241309/145‑1 (MQ=255)                                              tCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCGTTTTTTTTTTTGCATCGGGCTACGGCTTGTCTTcc                                                                                                              >  1:241309/1‑145 (MQ=255)                                                     tAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACCCGAAAGAGTGGCG‑ttttt                                                                                                                                           <  3:246346/108‑1 (MQ=38)                                                         cGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCAt                                                                                              <  2:40254/149‑1 (MQ=255)                                                            ttCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCgg                                                                                                                               >  2:249024/1‑113 (MQ=255)                                                            ttCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCgg                                                                                                                               <  1:249024/113‑1 (MQ=255)                                                               ggCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGa                                                                                         >  2:196776/1‑148 (MQ=255)                                                                gCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGaaa                                                                                       >  4:304568/1‑149 (MQ=255)                                                                  gTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAAtt                                                                                     <  7:147223/149‑1 (MQ=255)                                                                    cTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATAGGGCTACTGCTTGTGTTCCAGTTGGGCTGCCGCATCGGGAGAttt                                                                                    >  7:154958/1‑148 (MQ=255)                                                                      tttttCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑‑TTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCaa                                                                                 <  4:252231/148‑1 (MQ=255)                                                                                  gTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCggg                                                                                                                              <  2:22792/92‑1 (MQ=255)                                                                                  gTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCggg                                                                                                                              >  1:22792/1‑92 (MQ=255)                                                                                     aaaGCCATCGGGATCCATGGGCATCACCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTAtc                                                                  <  5:65036/149‑1 (MQ=255)                                                                                         ccATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGGATCGGGCTACGGCTTGTCTTCCAGTTTGGCTGCCGCATCGGGAAATTTCAACCAGCGCAGCTTAtcgtc                                                               >  3:262983/1‑148 (MQ=255)                                                                                          cATCGGGATCCATGGGCATCACCACATTTGGCTTCACTGCAAAAAAAGCCACACTTTCGGGTGCCGTTTTTTTTTTTGCATCGGGCAACGCATTGAGAACCAGTTCTGGTGCCGCAACGGGAAATTTAAACCATCTCATCTTAtcctc                                                               >  8:89745/1‑148 (MQ=255)                                                                                           aTCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGC‑AAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTAAGGCTTGTCTTCCAGTTGGGCTGCCGCATGGGGAAATTTCAAACCGCTAAGATTAACGTCGAAc                                                           >  6:193200/1‑149 (MQ=255)                                                                                               gcaTCCATGGGCATCCCCACATTTGGCTTCACTCCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGac                                                        <  5:172115/147‑1 (MQ=255)                                                                                                gATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCCGGAAATTTCAACCAGCTCAGCTTa                                                                    >  7:267039/1‑136 (MQ=255)                                                                                                gATCCATGGGCATCCACACATTTGGCTTCACTTCAAAAAACGCCACTGTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTa                                                                    <  8:267039/136‑1 (MQ=255)                                                                                                         gCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGTATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACcagctcag                                                                        <  1:100017/123‑1 (MQ=255)                                                                                                         gCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACcagctcag                                                                        >  2:100017/1‑123 (MQ=255)                                                                                                            tCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGaca                                           <  5:115490/149‑1 (MQ=255)                                                                                                             ccccACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGacac                                          <  6:205565/149‑1 (MQ=255)                                                                                                              cccACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGacac                                          <  3:304568/148‑1 (MQ=255)                                                                                                              cccACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACAcc                                         >  8:322469/1‑149 (MQ=255)                                                                                                               ccACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACAccc                                        >  7:8179/1‑149 (MQ=255)                                                                                                                 acaTTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCg                                       >  8:186633/1‑148 (MQ=255)                                                                                                                         cTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATa                              <  7:322469/149‑1 (MQ=255)                                                                                                                                 cAAAAAACGCCACTCTTTCGGTTGGCT‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCTACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATTGTTGAtt                       <  5:343746/148‑1 (MQ=255)                                                                                                                                 cAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCAAAGCGCTACGGCTTGACTTCCAGTTGGGAAGCCGCATCGGGAAATTTCAACCAGCTCAGCTTAACGTCCAACGACACTAACACCGACACCCGGAACAGATAGTTGAtt                       >  6:343746/1‑148 (MQ=255)                                                                                                                                  aaaaaaCGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTATTCCAGTTGGGCTGCCGAATCGGGAAATTTCAACCAGCTAAGCTTATCGTCGAACGCCAATTCAACCGACACACGGAACTGAAAGTTGATTGc                     >  7:72892/1‑149 (MQ=255)                                                                                                                                  aaaaaaCGCCACCCGAAAGAGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGaa                                                            >  2:84585/1‑110 (MQ=38)                                                                                                                                    aaaaCGCCACTCTTTCGGGTTGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTTCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCa                   <  5:193200/149‑1 (MQ=255)                                                                                                                                         gCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGa                                                         <  6:245453/106‑1 (MQ=255)                                                                                                                                         gCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGa                                                         >  5:245453/1‑106 (MQ=255)                                                                                                                                             ctctTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCAGCCTCAACa          <  7:186633/149‑1 (MQ=255)                                                                                                                                                  tCGGGTTGCG‑TTTTTGTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCTCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCAGCCTCAACAGTTc      <  4:262983/148‑1 (MQ=255)                                                                                                                                                     ggTGGCT‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCAGCCTCAACAGTTCGGtt  <  1:196776/149‑1 (MQ=255)                                                                                                                                                     ggTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCAGCCTCAACAGTTCGGtt  <  8:8179/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GCTCCAAATCAAGATTTACAGCCTTCCACGGAGGTCTGTAGATCCTCGAAAATAGACGCTTCGGCGTCTTTTTTCGTTTCGGTCAAAGCCATCGGGATCCATGGGCATCCCCACATTTGGCTTCACTGCAAAAAACGCCACTCTTTCGGGTGGCG‑TTTTTTTTTTGCATCGGGCTACGGCTTGTCTTCCAGTTGGGCTGCCGCATCGGGAAATTTCAACCAGCTCAGCTTATCGTCGAACGACACTTCAACCGACACCCGGAACTGATAGTTGATTGCCAGCCTCAACAGTTCGGTT  >  NC_002947/2103196‑2103492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑