Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,109,859 C→G 8.1% G1476A (GGT→GCT)  PP_1882 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,109,8590CG 8.1% 144.3 / 2.5 47G1476A (GGT→GCT) PP_1882membrane protein
Reads supporting (aligned to +/- strand):  ref base C (21/22);  new base G (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGCTTCCGTTTGGAGCGCCGTACCGGTGACTGACAACGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATG  >  NC_002947/2109712‑2110005
                                                                                                                                                   |                                                                                                                                                    
aTCGCTTCCGTTTGGAGCGCCGTACCGGTGACTGACAACGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCAGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCAcc                                                                                                                                                     >  6:104572/1‑149 (MQ=255)
 tCGCTTCCGTTTGGAGCGCCGTACCGGTGACTGACAACGTGGCTGTAGCCACCGCGGCCGCCAACCTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACcg                                                                                                                                                    >  3:222833/1‑149 (MQ=255)
    cTTCCGTTTGGAGCGCCGTACCGGTGACTGACAACGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACcggcg                                                                                                                                                 >  3:87140/1‑149 (MQ=255)
     ttCCGTTTGGAGCGCCGTACCGGTGACTGACAACGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACcggcgg                                                                                                                                                <  8:284468/149‑1 (MQ=255)
       ccGTTTGGAGCGCCGTACCGGTGACTGACAACGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACcggcgg                                                                                                                                                <  8:70236/147‑1 (MQ=255)
                   ccGTACCGGTGACTGACAACGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACcggcgg                                                                                                                                                >  1:333628/1‑135 (MQ=255)
                   ccGTAACGGTGAATGACAACGTTTCTTTATCCACCGCGGCCTCCAACTTGATTCCGAATGCGAGCGATAATGTGGCAATTGCCCCTACACCTGCAGAAAGTGCCGCACCCGCGCCACTGATGCCGCCTcctgatt                                                                                                                                                <  2:333628/135‑6 (MQ=255)
                    cGTACCGGTGACTGACAACGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCc‑g                                                                                                                                >  5:352101/1‑149 (MQ=255)
                                  acAACGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCa                                                                                                                  <  4:87140/149‑1 (MQ=255)
                                    aaCGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCGGTggcggcatc                                                                                                                                            <  3:186439/122‑4 (MQ=255)
                                    aaCGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCGGTggcggcatc                                                                                                                                            <  7:251990/122‑4 (MQ=255)
                                    aaCGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCGGTggcggcatc                                                                                                                                            >  4:186439/1‑119 (MQ=255)
                                    aaCGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCGGTggcggcatc                                                                                                                                            >  8:251990/1‑119 (MQ=255)
                                    aaCGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACcggcg                                                                                                                                                 <  2:63308/117‑1 (MQ=255)
                                    aaCGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACcggcg                                                                                                                                                 >  1:63308/1‑117 (MQ=255)
                                    aaCGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑g                                                                                                                >  2:171060/1‑148 (MQ=255)
                                      cGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTa                                                                                                             <  5:104572/149‑1 (MQ=255)
                                       gTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACcggcg                                                                                                                                                 <  8:129202/114‑1 (MQ=255)
                                       gTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACcggcg                                                                                                                                                 >  7:129202/1‑114 (MQ=255)
                                         ggCTGTAGCCACCGCGGCCGCCAACCTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGc                                                                                                          <  4:222833/149‑1 (MQ=255)
                                              tAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCAc                                                                                                       <  5:125598/147‑1 (MQ=255)
                                                           cgccAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGc                                                                                                                                            >  2:343272/1‑99 (MQ=255)
                                                           cgccAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGc                                                                                                                                            <  1:343272/99‑1 (MQ=255)
                                                             ccAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGAcggcg                                                                                      <  2:241789/149‑1 (MQ=255)
                                                                aCTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCCCCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGcc                                                                                   >  8:225396/1‑149 (MQ=255)
                                                                      tGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGAtt                                                                                                                           <  2:333123/104‑1 (MQ=255)
                                                                             ggCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACg                                                                                <  6:162547/139‑1 (MQ=255)
                                                                             ggCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACg                                                                                >  5:162547/1‑139 (MQ=255)
                                                                                gagcgaTACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTgcgg                                                                   <  8:271820/149‑1 (MQ=255)
                                                                                    gaTACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTgcggcg                                                                 >  4:263627/1‑147 (MQ=255)
                                                                                         gCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTa                                                          >  5:147391/1‑149 (MQ=255)
                                                                                               aaTTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGcc                                                      >  8:37843/1‑147 (MQ=255)
                                                                                                 ttGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAgg                                                  <  6:147391/149‑1 (MQ=255)
                                                                                                   gCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGaa                                                               <  6:14754/134‑1 (MQ=255)
                                                                                                                   aaaGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGa                                                                              <  1:221976/103‑1 (MQ=255)
                                                                                                                   aaaGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGa                                                                              >  2:221976/1‑103 (MQ=255)
                                                                                                                    aaGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGa                               <  3:263627/149‑1 (MQ=255)
                                                                                                                           gCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCCGCC‑GCGCTTGCCGGCGCA‑GCTACGCCACCTGTCCCCCCGACGGCGGCCACGGAGGCTACTGCGGCGCAGCGAAAGCCCCGGCCTATCCCCGGCCAGCCGCTCGCAcc                        >  8:183381/1‑149 (MQ=255)
                                                                                                                                 gcgcCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCAACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCTGCCGATCGCACCTAAgga                  >  2:96070/1‑148 (MQ=255)
                                                                                                                                     cACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATc                                            <  7:162297/119‑1 (MQ=255)
                                                                                                                                     cACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATc                                            >  8:162297/1‑119 (MQ=255)
                                                                                                                                     cACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTc                                      <  7:294614/125‑1 (MQ=255)
                                                                                                                                     cACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTc                                      >  8:294614/1‑125 (MQ=255)
                                                                                                                                           tGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGc        <  6:352101/149‑1 (MQ=255)
                                                                                                                                              cgccACCGGCGGCTGCCCCGGCAGCC‑GCGATTGACGGCGCA‑GCTACGCCACCTGTAACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATTCCCAGGCCTATGCCCCTACAGCCGCTAGCCCCTAAGGCCTTGACCCGc        >  8:219536/1‑146 (MQ=255)
                                                                                                                                              cgccACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGc        >  5:213897/1‑146 (MQ=255)
                                                                                                                                               gccACCGGCGGCTGCCCAGGCAGCCGGCGATTGCCGGCGCA‑GCTATGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGc        >  1:181099/1‑146 (MQ=255)
                                                                                                                                                 cACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATg  <  7:219536/149‑1 (MQ=255)
                                                                                                                                                 cACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATg  <  6:213897/149‑1 (MQ=255)
                                                                                                                                                   ccGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCAGGCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTg              >  1:233478/1‑136 (MQ=255)
                                                                                                                                                   ccGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTg              <  2:233478/135‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATCGCTTCCGTTTGGAGCGCCGTACCGGTGACTGACAACGTGGCTGTAGCCACCGCGGCCGCCAACTTGATGCCGACGGCGAGCGATACGCTGGCAATTGCCCCGACACCTGCCGAAAGTGCCGCACCCGCGCCACTGATGCCGCCACCGGCGGCTGCCCAGGCAGCC‑GCGATTGCCGGCGCA‑GCTACGCCACCTGTCACCACGACGGCGGCCACGGAGGCAACTGCGGCGAAGAGTATGCCCAGGCCTATCCCCGTCCAGCCGATCGCACCTAAGGCCTTGCCCCGCCAGATG  >  NC_002947/2109712‑2110005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑