Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,147,208 T→G 14.1% intergenic (+81/+36) murB → / ← rne UDP‑N‑acetylenolpyruvoylglucosamine reductase/ribonuclease E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,147,2080TG14.1% 150.4 / 14.0 56intergenic (+81/+36)murB/rneUDP‑N‑acetylenolpyruvoylglucosamine reductase/ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (24/24);  new base G (5/3);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTT  >  NC_002947/2147069‑2147348
                                                                                                                                           |                                                                                                                                             
aGGCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTgg                                                                                                                                       <  4:82501/148‑1 (MQ=255)
 ggCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCg                                                                                                                                     >  8:66689/1‑149 (MQ=255)
     gaaatCTTCGAGCGGTTTGGGGGTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGc                                                                                                                                 <  3:330283/146‑1 (MQ=255)
         tCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCGGGGCtttt                                                                                                                             >  7:132055/1‑149 (MQ=255)
          cTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCGGGGCttt                                                                                                                              <  1:248407/147‑1 (MQ=255)
          cTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCGGGGCttt                                                                                                                              >  2:248407/1‑147 (MQ=255)
           ttCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCttttt                                                                                                                            >  6:319026/1‑148 (MQ=255)
             cGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCtttt                                                                                                                             <  6:222536/145‑1 (MQ=255)
              gAGTGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTttca                                                                                                                          <  6:5162/147‑1 (MQ=255)
              gAGTGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTttca                                                                                                                          >  5:5162/1‑147 (MQ=255)
               aGCGGTTTGGGGTTGAGCTGGAGATGGAGCATAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCGGGGCtttttttgg                                                                                                                        <  8:132055/148‑5 (MQ=38)
                    tttGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCgg                                                                                                                                    <  4:193747/131‑1 (MQ=255)
                    tttGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCgg                                                                                                                                    >  3:193747/1‑131 (MQ=255)
                    tttGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCtt                                                                                                                  >  6:305470/1‑149 (MQ=255)
                                 tGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGcc                                                                                                      >  5:25723/1‑148 (MQ=255)
                                   gagaTGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGcc                                                                                                      <  6:25723/146‑1 (MQ=255)
                                       tGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGTTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGTCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGt‑gatga                                                                                              <  8:33422/149‑1 (MQ=255)
                                       tGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGAAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGt‑gatga                                                                                              <  5:305470/149‑1 (MQ=255)
                                          aGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGt                                                                                           >  2:280287/1‑149 (MQ=255)
                                            ccTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGttt                                                                                         <  1:280287/149‑1 (MQ=255)
                                                aTCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGtt                                                                                                                                               <  2:45127/92‑1 (MQ=255)
                                                aTCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGtt                                                                                                                                               >  1:45127/1‑92 (MQ=255)
                                                aTCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGc                                                                                     >  2:75047/1‑149 (MQ=255)
                                                aTCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGAGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGc                                                                                     >  3:295379/1‑149 (MQ=255)
                                                  cTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCt                                                                                    >  5:356795/1‑148 (MQ=255)
                                                    ttACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAg                                                                                                                     <  1:143105/114‑1 (MQ=255)
                                                    ttACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAg                                                                                                                     >  2:143105/1‑114 (MQ=255)
                                                               ccGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GACGAGGTTTGTGCTCATCGGCGGCTTc                                                                       >  1:251190/1‑148 (MQ=255)
                                                                cGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCa                                                                     <  1:75047/149‑1 (MQ=255)
                                                                      tgAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATcggcg                                                                            <  1:334847/136‑1 (MQ=255)
                                                                      tgAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATcggcg                                                                            >  2:334847/1‑136 (MQ=255)
                                                                          cccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTtg                                                                                        <  3:54974/120‑1 (MQ=255)
                                                                          cccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCt                                                                >  5:144880/1‑144 (MQ=255)
                                                                          cccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCt                                                                <  6:144880/144‑1 (MQ=255)
                                                                          cccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGATTTGGCTATCAGCCGT‑GATGAGGTTtg                                                                                        >  4:54974/1‑120 (MQ=255)
                                                                             ccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTg                                                        <  5:319026/149‑1 (MQ=255)
                                                                                  ggAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGaa                                                    <  4:295379/148‑1 (MQ=255)
                                                                                     gCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGACGGCTTCCAGGGCTGGTTCCTGGGAAGCTg                                                <  6:356795/149‑1 (MQ=255)
                                                                                      cGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCt                                                                >  2:8525/1‑132 (MQ=255)
                                                                                      cGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCt                                                                <  1:8525/132‑1 (MQ=255)
                                                                                        ggTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgcag                                             <  7:66689/149‑1 (MQ=255)
                                                                                             aCCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCt                                        >  8:141967/1‑149 (MQ=255)
                                                                                                            cGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTg                         >  2:301146/1‑149 (MQ=255)
                                                                                                             gTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTgg                        <  7:141967/149‑1 (MQ=255)
                                                                                                                  gCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGgcagca                   <  7:65141/149‑1 (MQ=255)
                                                                                                                  gCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGgcag                     >  2:73931/1‑147 (MQ=255)
                                                                                                                  gCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGgcag                     >  8:65141/1‑147 (MQ=255)
                                                                                                                     aaCCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GACGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGcc                <  2:251190/149‑1 (MQ=255)
                                                                                                                              aaGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCgg            >  7:13139/1‑144 (MQ=255)
                                                                                                                              aaGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCgg            <  8:13139/144‑1 (MQ=255)
                                                                                                                                  cccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCt   >  2:238088/1‑149 (MQ=255)
                                                                                                                                  cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGcc          >  3:120874/1‑142 (MQ=255)
                                                                                                                                  cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGcc          <  4:120874/142‑1 (MQ=255)
                                                                                                                                  cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCt   >  7:274686/1‑149 (MQ=255)
                                                                                                                                  cccGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCt   >  3:147968/1‑149 (MQ=255)
                                                                                                                                   ccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCtg                                   >  3:285441/1‑116 (MQ=255)
                                                                                                                                   ccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCtg                                   <  4:285441/116‑1 (MQ=255)
                                                                                                                                    cGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCtt  >  1:87743/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                             
AGGCGGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGT‑GATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTT  >  NC_002947/2147069‑2147348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑