Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,157,948 C→A 10.5% R100S (CGC→AGC)  fabG → 3‑oxoacyl‑ACP reductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,157,9480CA10.5% 104.4 / 2.1 36R100S (CGC→AGC) fabG3‑oxoacyl‑ACP reductase subunit
Reads supporting (aligned to +/- strand):  ref base C (15/17);  new base A (2/2);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCATTACCGGCACTGGCATGGAGCTGAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGACCAAGGCGCGTTGGGGTCGTATCATCAGCATCGGCTCGGTCGTTGGTGCCAT  >  NC_002947/2157807‑2158087
                                                                                                                                             |                                                                                                                                           
ggCATTACCGGCACTGGCATGGAGCTGAACGTAACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGa                                                                                                                                       >  8:305279/1‑148 (MQ=255)
 gCATTACCGGCACTGGCATGGAGCTGAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCGCAGCATGagg                                                                                                                                     <  1:195333/149‑3 (MQ=255)
  cATTACCGGCACTGGCATGGAGCTGAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAg                                                                                                                                    <  1:280776/149‑1 (MQ=255)
          gCACTGGCATGGAGCTGAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAga                                                                                                                                   <  1:30289/142‑1 (MQ=255)
          gCACTGGCATGGAGCTGAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAga                                                                                                                                   >  2:30289/1‑142 (MQ=255)
           cACTGGCATGGAGCTGAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGAcgacgatt                                                                                                                           <  7:229338/149‑3 (MQ=255)
           cACTGGCATGGAGCTGAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGt                                                                                                                           <  4:201063/149‑1 (MQ=255)
                gCATGGAGCTGAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGtt                                                                                                                       <  1:323348/148‑1 (MQ=255)
                          gAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCGCAGCATGag                                                                                                                                      >  4:241151/1‑122 (MQ=255)
                          gAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCGCAGCATGag                                                                                                                                      <  3:241151/123‑2 (MQ=255)
                                                gTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCAt                                                                                                              >  5:45994/1‑125 (MQ=255)
                                                gTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCAt                                                                                                              <  6:45994/125‑1 (MQ=255)
                                                     aGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGt                                                                                  >  2:210992/1‑148 (MQ=255)
                                                     aGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGt                                                                                  <  1:210992/148‑1 (MQ=255)
                                                         gTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACCCCAACCTGAACAGCCTCTACCGTCTGTc                                                                             >  1:233953/1‑149 (MQ=255)
                                                             tGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCGAg                                                                         >  7:76405/1‑149 (MQ=255)
                                                               gccgccATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAggg                                                                       >  1:47539/1‑149 (MQ=255)
                                                                 cgccATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCctc                                                                                        <  2:243006/130‑1 (MQ=255)
                                                                 cgccATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCctc                                                                                        >  1:243006/1‑130 (MQ=255)
                                                                 cgccATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGt                                                                                  <  6:223228/136‑1 (MQ=255)
                                                                 cgccATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGAGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGt                                                                                  >  5:223228/1‑136 (MQ=255)
                                                                          cGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTg                                                            >  2:31820/1‑149 (MQ=255)
                                                                           gAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGaaa                                                                                                                                     >  8:301963/1‑75 (MQ=255)
                                                                            aGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCGAGGGCGTGCTGCGTGGc                                                          <  8:76405/149‑1 (MQ=255)
                                                                                          ccGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTg                                                            >  6:295604/1‑133 (MQ=255)
                                                                                          ccGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTg                                                            <  5:295604/133‑1 (MQ=255)
                                                                                           cGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGACCAAGGCGCGt                                           <  7:305279/149‑1 (MQ=255)
                                                                                                                ccGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGACCAAGGCGCGTTGGGGTCGTATCATCAGCAt                       <  2:233953/148‑1 (MQ=255)
                                                                                                                   gTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACAGTCTGTACAAGGGCGTGCTGAGTGGCATGACCAAGGCGCGTTGGGGTCGTATCATCAGCATCGGc                   >  5:9868/1‑149 (MQ=255)
                                                                                                                      tCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGACCAAGGCGCGTTGGGGTCGTATCATCAGCATCGGCTCg                >  1:111433/1‑149 (MQ=255)
                                                                                                                        aCCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGACCAAGGCGCGTTGGGGTCGTATCATCAGCATCGGCTCGGt              <  6:9868/149‑1 (MQ=255)
                                                                                                                         cccGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGAc                                                     <  7:83552/109‑1 (MQ=255)
                                                                                                                         cccGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGAc                                                     >  8:83552/1‑109 (MQ=255)
                                                                                                                              gACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGACCAAGGCGCGTTGGGGTCGTATCATCAGCATCGGCTCGGTCGTTgg        >  1:103202/1‑149 (MQ=255)
                                                                                                                                   ccTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGACCAAGGCGCGTTGGGGTCGTATCATCAGCATCGGCTCGGTCGTTGGTGCCa   <  1:31820/149‑1 (MQ=255)
                                                                                                                                    cTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGACCAAGGCGCGTTGGGGTCGTATCATCAGCATCGGCTCGGTCGTTGGTGCCAt  <  2:111433/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
GGCATTACCGGCACTGGCATGGAGCTGAACGTGACCAGCGCCGAATCGGTTGAAGCCGTACTGGCCGCCATTGGCGAGCAGTTCGGCGCGCCGGCCATCTTGGTCAACAATGCCGGTATCACCCGCGACAACCTCATGCTGCGCATGAAAGACGACGAGTGGTTTGATGTCATCGACACCAACCTGAACAGCCTCTACCGTCTGTCCAAGGGCGTGCTGCGTGGCATGACCAAGGCGCGTTGGGGTCGTATCATCAGCATCGGCTCGGTCGTTGGTGCCAT  >  NC_002947/2157807‑2158087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑