Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,160,919 C→A 8.2% C219* (TGC→TGA) ‡ pabC → 4‑amino‑4‑deoxychorismate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,160,9190CA 8.2% 150.1 / 4.4 49C219* (TGC→TGA) ‡pabC4‑amino‑4‑deoxychorismate lyase
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

GTGATCGAAGGGGTATACAGCAATCTGTTTCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGCCCGCTCACTCGTAAACTGCAGGCCGTTGCCCGTACGTTAC  >  NC_002947/2160773‑2161067
                                                                                                                                                  |                                                                                                                                                    
gTGATCGAAGGGGTATACAGCAATCTGTTTCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGa                                                                                                                                                    >  6:338837/1‑149 (MQ=255)
      gAAGGGGTTTACAGCAATCTGTTTCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCTGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGt                                                                                                                                               <  8:294625/148‑1 (MQ=255)
       aaGGGGTATACAGCAATCTGTTTCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCg                                                                                                                                             >  3:30372/1‑149 (MQ=255)
             tctaCAGCAATCGGTTTCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGGGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTTGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAg                                                                                                                                       <  7:235944/147‑1 (MQ=255)
                     aaTCTGTTTCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGa                                                                                                                                        <  6:34272/140‑1 (MQ=255)
                     aaTCTGTTTCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGa                                                                                                                                        >  5:34272/1‑140 (MQ=255)
                      aTCTGTTTCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAg                                                                                                                              <  6:174687/149‑1 (MQ=255)
                           tttCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAg                                                                                                                          <  4:219786/148‑1 (MQ=255)
                           tttCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCGCAGACCTGtacc                                                                                                                                            >  6:294063/1‑127 (MQ=255)
                           tttCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCGCAGACCTGtacc                                                                                                                                            <  5:294063/130‑4 (MQ=255)
                               tGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAAt                                                                                                                     >  4:232510/1‑149 (MQ=255)
                               tGGGGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTTGAACAGGGCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTGGAGGCGCGGGAGCAAGCGAAt                                                                                                                     <  6:309309/149‑1 (MQ=255)
                                 gTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAAt                                                                                                                     <  5:81659/147‑1 (MQ=255)
                                 gTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATg                                                                                                                    >  8:178052/1‑148 (MQ=255)
                                 gTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGa                                                                                                                   <  4:30372/149‑1 (MQ=255)
                                  tGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCGCAGACCTGtacc                                                                                                                                            <  1:17494/123‑4 (MQ=255)
                                  tGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCGCAGACCTGtacc                                                                                                                                            >  2:17494/1‑120 (MQ=255)
                                        aTGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTAttt                                                                                                            <  7:178052/149‑1 (MQ=255)
                                                  gctTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTAGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCaa                                                                                                    >  2:268137/1‑147 (MQ=255)
                                                               cTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCAt                                                                                     >  1:258246/1‑149 (MQ=255)
                                                                  aGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGAACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTg                                                                                  >  1:227307/1‑149 (MQ=255)
                                                                   gCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTgg                                                                                 <  3:232510/149‑1 (MQ=255)
                                                                          tggcgtggcgGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCcgtg                                                                           <  2:258246/148‑1 (MQ=255)
                                                                                       gTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCcgc                                                             >  1:130822/1‑149 (MQ=255)
                                                                                         gATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTAtttgttt                                                                                                        <  1:288593/104‑1 (MQ=255)
                                                                                         gATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTAtttgttt                                                                                                        >  2:288593/1‑104 (MQ=255)
                                                                                          aTGCGTTCCGCACTGCGGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCgcgc                                                           <  2:227307/148‑1 (MQ=255)
                                                                                                 ccGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTg                                                    <  5:338837/148‑1 (MQ=255)
                                                                                                 ccGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTg                                                    <  6:304318/148‑1 (MQ=255)
                                                                                                         ctgGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGc                                                                                                                         >  7:1523/1‑71 (MQ=255)
                                                                                                         ctgGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGc                                                                                                                         <  8:1523/71‑1 (MQ=255)
                                                                                                         ctgGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCggg                                           >  5:155941/1‑149 (MQ=255)
                                                                                                           ggAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGt                                                                   <  2:332429/123‑1 (MQ=255)
                                                                                                           ggAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGt                                                                   >  1:332429/1‑123 (MQ=255)
                                                                                                           ggAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGcc                                         >  6:106798/1‑149 (MQ=255)
                                                                                                             aaCAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGTTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGCCCg                                       >  1:95995/1‑149 (MQ=255)
                                                                                                               cAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGCCCGCt                                     <  6:155941/149‑1 (MQ=255)
                                                                                                                    cccGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGc                                                                                >  2:63536/1‑101 (MQ=255)
                                                                                                                    cccGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGc                                                                                <  1:63536/101‑1 (MQ=255)
                                                                                                                     ccGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGCCCGCTCACTc                                <  6:360547/148‑1 (MQ=255)
                                                                                                                     ccGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGCCCGCTCACTc                                >  5:360547/1‑148 (MQ=255)
                                                                                                                               gTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGCCCGCTCACTCGTAAACTGCAg                     <  2:130822/149‑1 (MQ=255)
                                                                                                                                 aTTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCcgc                                                             <  3:190121/107‑1 (MQ=255)
                                                                                                                                 aTTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCcgc                                                             >  4:190121/1‑107 (MQ=255)
                                                                                                                                           aGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGCCCGCTCACTCGTAAACTGCAGGCCGTTGCCCg          >  4:177941/1‑148 (MQ=255)
                                                                                                                                           aGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGCCCCGCTCACTCGTAAACTGCAGGCCGTTGCCCg          >  8:86567/1‑148 (MQ=255)
                                                                                                                                             gTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTAc                                                                                          >  1:313736/1‑66 (MQ=255)
                                                                                                                                             gTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTAc                                                                                          <  2:313736/66‑1 (MQ=255)
                                                                                                                                             gTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGCCCGCTCACTCGTAAACTGCAGGCCGTTGCCCGTAc       >  4:102906/1‑149 (MQ=255)
                                                                                                                                                  cGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGCCCGCTCACTCGTAAACTGCAGGCCGTTGCCCGTACGTTAc  >  2:5825/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTGATCGAAGGGGTATACAGCAATCTGTTTCTGGTGCGCGATGGCGTGCTGCTTACCGCCGACCTTAGCCGTTGTGGCGTGGCGGGCGTGATGCGTGCCGCACTGCTGGAACAGGCCCCGCTGCTGGGTATTGCGGTACAGGTCTGCGACCTGTCGTTCGAGGCGCTGGAGCAAGCGAATGAAGTATTTGTTTGCAATAGCGTTTACGGCATTTGGCCCGTGCGTGGCGTAGCCGCGCTAAACTGGTCGCCGGGCCCGCTCACTCGTAAACTGCAGGCCGTTGCCCGTACGTTAC  >  NC_002947/2160773‑2161067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑