Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,171,503 G→A 9.2% intergenic (+38/+365) PP_5484 → / ← PP_1924 hypothetical protein/phosphinothricin N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,171,5030GA 9.2% 147.3 / 4.4 54intergenic (+38/+365)PP_5484/PP_1924hypothetical protein/phosphinothricin N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base A (3/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

AGAATTACTACTTTGCTCGACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGACAGCTCTCCCGCCGAGACAGCTGGCTATCCCCACTTGGCT  >  NC_002947/2171368‑2171646
                                                                                                                                       |                                                                                                                                               
ataaTTACTACTTTGCTCGTCAGCTTTCGGCTGCACCCGTGATTTGGGGGTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTAt                                                                                                                                    <  8:159212/147‑1 (MQ=255)
    ttACTACTTTGCTCGACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAg                                                                                                                                >  6:211114/1‑149 (MQ=255)
          ctTTGCTCGACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCCAAGAGCggg                                                                                                                                          <  2:220460/133‑1 (MQ=255)
          ctTTGCTCGACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCCAAGAGCggg                                                                                                                                          >  1:220460/1‑133 (MQ=255)
           tttGCTCGACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTggggg                                                                                                                          >  8:137163/1‑148 (MQ=255)
           tttGCTCGACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGt                                                                                                                         >  4:327252/1‑149 (MQ=255)
             tGCTCGACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTa                                                                                                                        <  4:97454/148‑1 (MQ=255)
                tCGACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGc                                                                                                                    >  8:146949/1‑149 (MQ=255)
                  gACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGc                                                                                                                                             >  2:17925/1‑122 (MQ=255)
                  gACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGc                                                                                                                                             <  1:17925/122‑1 (MQ=255)
                  gACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCgg                                                                                                                  <  5:211114/149‑1 (MQ=255)
                   ctatcTTTCGGCTGCACCCGTGATTTGGGGTTGCGGCATTTCACAAATCAGCTGCCTTTTCCCGGTTCGCCTGTACTGATTGACGACATGGGTATGCGAAGTTTCGACCCTCTCTTGGTGCGGGTTTTAAGCAGTGGGGGTAGGGCggg                                                                                                                 <  4:90069/145‑1 (MQ=255)
                   aCAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCggg                                                                                                                 >  2:150441/1‑149 (MQ=255)
                   aCAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCggg                                                                                                                 <  1:150441/149‑1 (MQ=255)
                        tttCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGc                                                                                                                    >  1:43834/1‑141 (MQ=255)
                        tttCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGc                                                                                                                    <  2:43834/141‑1 (MQ=255)
                        tttCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAac                                                                                                            >  1:226064/1‑149 (MQ=255)
                        tttCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTAGGAGCGGCATTTATCCAGTGGGGGTAGGGCggtgagat                                                                                                            >  3:90069/1‑143 (MQ=255)
                             gCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAacgac                                                                                                         >  6:262352/1‑147 (MQ=255)
                             gCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCCAAGAGCgggtc                                                                                                                                        <  8:215941/116‑3 (MQ=255)
                             gCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCCAAGAGCgggtc                                                                                                                                        >  7:215941/1‑114 (MQ=255)
                                    ccGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGtt                                                                                                <  5:262352/149‑1 (MQ=255)
                                    ccGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGtt                                                                                                <  4:150474/149‑1 (MQ=255)
                                    ccGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGt                                                                                                 >  1:34971/1‑148 (MQ=255)
                                    ccGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGt                                                                                                 >  3:150474/1‑148 (MQ=255)
                                               ggTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGCTAACGACGTGCCGGTTACGTGCTCttt                                                                                     <  7:146949/149‑1 (MQ=255)
                                                gTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTTATTGACGACATGGGTAGGCGATGTTTCGACCCGCTCTTGGAGCGGTTTTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTa                                                                                    <  3:327252/149‑1 (MQ=255)
                                                    cGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCgggg                                                                                                                >  5:279375/1‑117 (MQ=255)
                                                    cGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCgggg                                                                                                                <  6:279375/117‑1 (MQ=255)
                                                      ccAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAg                                                                              >  8:203842/1‑149 (MQ=255)
                                                       cAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATTCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGc                                                                             <  1:265555/149‑1 (MQ=255)
                                                            cacaATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTATGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAaca                                                                         <  1:78879/148‑1 (MQ=255)
                                                             acaATTCAGCTGCATTTTCCCTAAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGGTACGTGCTCTTTATGTCAGCAACAcc                                                                       >  7:222900/1‑149 (MQ=255)
                                                                    cgccgCATTTTCCCTGCCGGCCGGTACTGTTTGACCACATGGGTCGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCCGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGAtt                                                                 <  8:222900/144‑1 (MQ=255)
                                                                    aGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGAtt                                                                 <  7:137163/148‑1 (MQ=255)
                                                                          aTTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTc                                                          <  2:34971/149‑1 (MQ=255)
                                                                           ttttCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGt                                                                                            <  8:65212/114‑1 (MQ=255)
                                                                           ttttCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCGTGGAGCGGGATTTATACAGTGGGGGTAGGGCGGGGTAACGACGTGGCGGTTACGTGCTGTTTATGTCAGCACCCCCCGGCTTGAGCGtgc                                                         >  8:260398/1‑147 (MQ=255)
                                                                             ttCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGc                                                        <  2:308752/148‑1 (MQ=255)
                                                                                ccTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATg                                                    <  7:203842/149‑1 (MQ=255)
                                                                                           gTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGa                                          >  8:106864/1‑148 (MQ=255)
                                                                                                     acgacATGGGTAGGCGAAGTTTCGCCCCGCTCTTGGAGAGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGACGACAGCTCTCccg                               >  8:164167/1‑149 (MQ=255)
                                                                                                         cATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGc                                                             <  6:325636/115‑1 (MQ=255)
                                                                                                         cATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGc                                                             >  5:325636/1‑115 (MQ=255)
                                                                                                                    gAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGACAGCTCTCCCGCCGAGACAGCTGGc                 >  2:232932/1‑148 (MQ=255)
                                                                                                                     aaGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGACAGCTCTCCCGCCGAGACAGCTGGCTa               >  1:219610/1‑149 (MQ=255)
                                                                                                                             aCCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGACAGCTCTCCCGCCGAGACAGCTGGCTATcc            <  8:78934/144‑1 (MQ=255)
                                                                                                                             aCCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGACAGCTCTCCCGCCGAGACAGCTGGCTATcc            >  7:78934/1‑144 (MQ=255)
                                                                                                                              cccGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGACAGCTCTCCCACCGAGACAGCTGGCTATCCCCACtt      >  8:104017/1‑149 (MQ=255)
                                                                                                                              cccGCTCCAAGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGACAGCTCTCCCGCCGAGACAGCTGGCTATCCCCACtt      >  3:83079/1‑149 (MQ=255)
                                                                                                                                cGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGACAGCTCTCCCACCGAGACAGCTGGCTATCCCCACTTgg    <  7:104017/149‑1 (MQ=255)
                                                                                                                                  ctctTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGACAGCTCTCCCGCCGAGACAGCTGGCTATCCCCACTTGGCt  <  2:226064/149‑1 (MQ=255)
                                                                                                                                     ttGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCttt                                                                                     <  6:101389/63‑1 (MQ=255)
                                                                                                                                     ttGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCttt                                                                                     >  5:101389/1‑63 (MQ=255)
                                                                                                                                       |                                                                                                                                               
AGAATTACTACTTTGCTCGACAGCTTTCGGCTGCACCCGTGATTTGGGGTTCCGCCAGTTCACAATTCAGCTGCATTTTCCCTGAGCGCCGGTACTGATTGACGACATGGGTAGGCGAAGTTTCGACCCGCTCTTGGAGCGGGATTTATCCAGTGGGGGTAGGGCGGGGTAACGACGTGCCGGTTACGTGCTCTTTATGTCAGCAACACCCGGATTGAGCGTCGCCATGCAATAGCCGACAGCTCTCCCGCCGAGACAGCTGGCTATCCCCACTTGGCT  >  NC_002947/2171368‑2171646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑