Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	204,738	C→G	44.2%	T3415S (ACC→AGC)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	204,738	0	C	G	44.2%	‑0.5 / 5.1	9	T3415S (ACC→AGC)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base G (2/2);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTC  >  NC_002947/204709‑204878                              |                                                                                                                                             ccAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGt                       <  4:208555/149‑1 (MQ=32)       tcagggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGTctgctg                 >  8:76473/7‑149 (MQ=255)           ggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTgg              <  1:194164/146‑1 (MQ=255)           ggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTgg              >  2:194164/3‑148 (MQ=255)               aCGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATg           <  7:332751/147‑1 (MQ=40)               aCGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATg           <  8:271206/147‑1 (MQ=40)               aCGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTa         <  7:76473/149‑1 (MQ=255)                    cTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACgg    >  1:311003/1‑149 (MQ=255)                      ggTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTc  >  8:203193/1‑149 (MQ=255)                              |                                                                                                                                             CCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCGTACACCCACGCTGCTCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTC  >  NC_002947/204709‑204878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑