Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,238,142 A→T 16.1% F148I (TTC→ATC)  PP_1975 ← EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,238,1420AT16.1% 70.3 / 5.2 31F148I (TTC→ATC) PP_1975EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base A (12/14);  new base T (3/2);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTCAAGCCCGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAGGCCATCGAGCACAGCA  >  NC_002947/2237998‑2238288
                                                                                                                                                |                                                                                                                                                  
tctcAAGCCCGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATc                                                                                                                                                >  7:96146/1‑149 (MQ=255)
              aaCAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGATGTTCGCCATga                                                                                                                                         >  4:152898/1‑141 (MQ=255)
              aaCAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGATGTTCGCCATga                                                                                                                                         <  3:152898/142‑2 (MQ=255)
                 aaGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGcgcg                                                                                                                               >  2:216009/1‑149 (MQ=255)
                    ccGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCggtg                                                                                                                            >  4:197969/1‑149 (MQ=255)
                        cGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGc                                                                                                                        <  4:219966/149‑1 (MQ=255)
                           tCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCg                                                                                                                     <  3:197969/149‑1 (MQ=255)
                                                tatTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAgg                                                                                                <  1:7562/149‑1 (MQ=255)
                                                         agaagaTGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGcc                                                                                       >  4:13519/1‑149 (MQ=255)
                                                             gaTGTATTCCCAGGCCGAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGAGCggtg                                                                                                                            <  8:107580/108‑1 (MQ=255)
                                                             gaTGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGACGCCCAGGGTGCGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGATCATCGCCATCCGTTAGGCGCggtg                                                                                                                            >  7:107580/1‑108 (MQ=255)
                                                                 tATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGAt                                                                               <  1:216009/149‑1 (MQ=255)
                                                                  aTTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGcc                                                                                       >  6:356688/1‑140 (MQ=255)
                                                                  aTTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGcc                                                                                       <  5:356688/140‑1 (MQ=255)
                                                                  aTTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGAt                                                                               <  8:96146/148‑1 (MQ=255)
                                                                            ccAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCGGGGTGAAGGCCAGCGGGATCAGCGCGCCAc                                                                    >  1:29580/1‑149 (MQ=255)
                                                                                     ggTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGGAGGCCAGCgg                                                                                  >  2:238746/1‑126 (MQ=255)
                                                                                     ggTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGGAGGCCAGCgg                                                                                  <  1:238746/126‑1 (MQ=255)
                                                                                      gTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCAGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAAcc                                                          >  1:250725/1‑149 (MQ=255)
                                                                                           ccagccGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGc                                                                                                      <  1:334004/100‑1 (MQ=255)
                                                                                           ccagccGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGc                                                                                                      >  2:334004/1‑100 (MQ=255)
                                                                                                    cAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACgg                                             >  6:343649/1‑148 (MQ=255)
                                                                                                       ggtgggtCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACa                                         <  6:62546/149‑1 (MQ=255)
                                                                                                           ggtCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCggg                                                                                                                   <  1:255944/71‑1 (MQ=255)
                                                                                                           ggtCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCggg                                                                                                                   >  2:255944/1‑71 (MQ=255)
                                                                                                                        gggCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAgg                        <  3:13519/149‑1 (MQ=255)
                                                                                                                              aaGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAg                  <  3:328481/149‑1 (MQ=255)
                                                                                                                                  caaaggcATGGCGATGTTCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATg                                 >  2:171624/7‑130 (MQ=255)
                                                                                                                                  caaaggcATGGCGATGTTCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATg                                 <  1:171624/124‑1 (MQ=255)
                                                                                                                                            gCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGCTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAGGCCATCGAGCAcag    >  3:27752/1‑149 (MQ=255)
                                                                                                                                              gAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCGGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAGGCCATCGAGCAcagca  <  2:29580/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TCTCAAGCCCGTATAACAAGCCGGCGATCATTAGCAGGCCCGGTGGGATATTGATGTAGAAGATGTATTCCCAGCCCCAGTTCTCGGTCAGCCAGCCGCCCAGGGTGGGTCCGATGGAGGGGGCAAAAGTGGCGGTCATGGCGAACATCGCCATGCCTTTGGCGCGGTGGTGCTCGGGCAGCTTGATCAGCGTCAGGGTGAAGGCCAGCGGGATCAGCGCGCCACCGGTGAAACCCTGCAAGGCACGGAACAGGATCATGCTCTCCAGGTTCCAGGCCATCGAGCACAGCA  >  NC_002947/2237998‑2238288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑