Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,241,568 G→C 10.8% noncoding (35/76 nt) PP_t25 → tRNA‑Ala

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,241,5680GC10.8% 102.9 / 5.4 37noncoding (35/76 nt)PP_t25tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base G (19/14);  new base C (2/2);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GTTTGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCT  >  NC_002947/2241428‑2241715
                                                                                                                                             |                                                                                                                                                   
gTTTGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCagg                                                                                                                                             <  7:283221/149‑1 (MQ=255)
 tttGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCag                                                                                                                                              >  2:248653/1‑147 (MQ=255)
  ttGACATACCTGCAATCCGTACTTAATATGCGCCTAGTCCAGATCGATGAACGAAAACGAAACTCCAGGCACCCAGCCATCGATTCAGTTGA‑AAGCGACATTGTGGGGCTATAGCTGATCTGGTAGAGCGCATGCATGCAATGGaggc                                                                                                                                            <  4:269705/148‑2 (MQ=255)
   tGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCAGGGAGAGCGCCTGCATGGCATGCaggag                                                                                                                                           <  1:194148/148‑1 (MQ=255)
         cccTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGc                                                                                                                                     <  6:190309/148‑1 (MQ=255)
         cccTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGc                                                                                                                                     >  5:190309/1‑148 (MQ=255)
               aaTCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGa                                                                                                                              >  1:22061/1‑149 (MQ=255)
                                 gcCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccacc                                                                                                             >  5:238485/1‑148 (MQ=255)
                                            aGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCt                                                                                                   >  5:135505/1‑147 (MQ=255)
                                                        aaacgaaacgCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCt                                                                                     >  3:273833/1‑149 (MQ=255)
                                                           cgaaacgCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGAt                                                                                  <  2:22061/149‑1 (MQ=255)
                                                            gaaacgCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGAt                                                                                  >  7:261412/1‑148 (MQ=255)
                                                             aaacgCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGAt                                                                                  >  1:16766/1‑147 (MQ=255)
                                                                cgCCAGGCACCCAGCCAGCGATTCAGTTCAGAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAg                                                                               >  1:208022/1‑148 (MQ=255)
                                                                     ggCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGAcc                                                                        <  8:261412/149‑1 (MQ=255)
                                                                     ggCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGAcc                                                                        >  2:17909/1‑149 (MQ=255)
                                                                       cACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCgg                                                                      <  2:208022/149‑1 (MQ=255)
                                                                              ccagcGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAg                                                                >  6:20959/1‑148 (MQ=255)
                                                                                   gATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCg                                                          >  4:115470/1‑149 (MQ=255)
                                                                                    aTTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCgg                                                         <  6:135505/149‑1 (MQ=255)
                                                                                        aGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCATAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTc                                                      <  1:248653/148‑1 (MQ=255)
                                                                                            cA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTg                                                   >  7:258153/1‑147 (MQ=255)
                                                                                               aaGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGCGTCTTGAAgg                                               >  4:214742/1‑149 (MQ=255)
                                                                                                          tgGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCAACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTc                                    >  7:3154/1‑149 (MQ=255)
                                                                                                          tgGGGCTATAGCTCAGCTGGGAGAGCGCCTACATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTc                                    <  6:169089/149‑1 (MQ=255)
                                                                                                                   aGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTc                           >  4:40791/1‑149 (MQ=255)
                                                                                                                      tCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTc                        >  2:336978/1‑149 (MQ=255)
                                                                                                                        aGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTc                        <  4:273833/147‑1 (MQ=255)
                                                                                                                        aGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTa                      <  2:16766/149‑1 (MQ=255)
                                                                                                                             ggAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTTAGAAGGTTCGTCCCCTTCGTCTAGTGGc                 <  8:258153/149‑1 (MQ=255)
                                                                                                                             ggAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTg                   <  1:17909/147‑1 (MQ=255)
                                                                                                                               agagCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAAc                                                            >  2:204357/1‑104 (MQ=255)
                                                                                                                               agagCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAAc                                                            <  1:204357/104‑1 (MQ=255)
                                                                                                                                   ctccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAg             >  4:141759/3‑147 (MQ=255)
                                                                                                                                   ctccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAg             <  3:141759/145‑1 (MQ=255)
                                                                                                                                    tccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGa           >  7:306435/2‑148 (MQ=255)
                                                                                                                                     ccTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGaca         >  4:245122/1‑149 (MQ=255)
                                                                                                                                        gcatggcatgCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGACACCg      >  6:15427/1‑149 (MQ=255)
                                                                                                                                        gcatggcatgCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGACACCg      >  5:318022/1‑149 (MQ=255)
                                                                                                                                         catggcatgCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGACAcc       <  3:40791/147‑1 (MQ=255)
                                                                                                                                           tggcatgCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGACACCGccc   <  6:318022/149‑1 (MQ=255)
                                                                                                                                            ggcatgCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCt  >  6:160062/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
GTTTGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCA‑AAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCT  >  NC_002947/2241428‑2241715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑