Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 237,450 G→A 10.0% D111N (GAT→AAT) ‡ algR → alginate biosynthesis regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947237,4500GA10.0% 137.9 / 4.8 50D111N (GAT→AAT) ‡algRalginate biosynthesis regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base A (3/2);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

CAGGTTGCCGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGATCTAT  >  NC_002947/237309‑237596
                                                                                                                                              |                                                                                                                                                  
cAGGTTGCCGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGACGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCtt                                                                                                                                             >  8:299929/1‑149 (MQ=255)
   gTTGCCGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTgcg                                                                                                                                          >  6:179414/1‑149 (MQ=255)
       ccGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCaa                                                                                                                                       <  5:146240/148‑1 (MQ=255)
       ccGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCAAGGCATCGCGCAAg                                                                                                                                      <  8:102875/149‑1 (MQ=255)
       ccGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGACGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAg                                                                                                                                      <  7:299929/149‑1 (MQ=255)
        cGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAgg                                                                                                                                     <  4:2475/149‑1 (MQ=255)
           tCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCAAGGCATCGCGCAAGGcgt                                                                                                                                  >  5:89034/1‑147 (MQ=255)
             gCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCAAGGCATCGCGCAAGGcgtgg                                                                                                                                <  6:89034/149‑5 (MQ=255)
                     gAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCaa                                                                                                                        >  6:213440/1‑149 (MQ=255)
                      aaCGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAAc                                                                                                                       <  3:62623/149‑1 (MQ=255)
                                             gtgtTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTAc                                                                                                <  5:179414/149‑1 (MQ=255)
                                               gtTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTAcc                                                                                               >  1:143468/1‑148 (MQ=255)
                                               gtTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTAcc                                                                                               <  2:143468/148‑1 (MQ=255)
                                                 tttGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACccgg                                                                                            >  7:72083/1‑149 (MQ=255)
                                                            gatgatGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGa                                                                                 <  1:48401/149‑1 (MQ=255)
                                                             atgatgAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATg                                                                                  >  7:220043/1‑147 (MQ=255)
                                                                atgaATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAATCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCaa                                                                                                                        >  1:265765/1‑106 (MQ=255)
                                                                atgaATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCaa                                                                                                                        <  2:265765/106‑1 (MQ=255)
                                                                     tACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAggcggcgg                                                                        >  5:32027/1‑149 (MQ=255)
                                                                     tACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCCTACCCGGCCGGCCAATGAAggcggcgg                                                                        >  8:225407/1‑149 (MQ=255)
                                                                             agagGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCACGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCCCg                                                                  >  8:76779/1‑147 (MQ=255)
                                                                             agagGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGCCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACg                                                                  >  3:78904/1‑147 (MQ=255)
                                                                              gagGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAg                                                               <  8:220043/149‑1 (MQ=255)
                                                                                ggCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGcc                                                             <  4:78904/149‑1 (MQ=255)
                                                                                   aTTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAAt                                                                                   <  3:8503/124‑1 (MQ=255)
                                                                                   aTTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAAt                                                                                   >  4:8503/1‑124 (MQ=255)
                                                                                   aTTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCata                                                          >  2:349238/1‑149 (MQ=255)
                                                                                   aTTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCata                                                          >  6:73945/1‑149 (MQ=255)
                                                                                               tACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCa                                              <  6:32027/149‑1 (MQ=255)
                                                                                                   cTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCTGCGATGCCTTGCGCAAGGCCGAAAAACCCAAc                                                                                                                       <  1:37909/73‑1 (MQ=255)
                                                                                                   cTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAAc                                                                                                                       >  2:37909/1‑72 (MQ=255)
                                                                                                    tCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCAcgcg                                          >  4:295114/1‑148 (MQ=255)
                                                                                                       gCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCaaa                                      <  1:349238/149‑1 (MQ=255)
                                                                                                           tgtgACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAggcggcgg                                                                        >  3:115277/1‑111 (MQ=255)
                                                                                                           tgtgACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAggcggcgg                                                                        <  4:115277/111‑1 (MQ=255)
                                                                                                            gtgACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCAt                                 >  6:85586/1‑149 (MQ=255)
                                                                                                                 cAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAg                                                      >  8:231865/1‑123 (MQ=255)
                                                                                                                 cAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc                            >  3:29974/1‑149 (MQ=255)
                                                                                                                 cAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc                            >  8:57849/1‑149 (MQ=255)
                                                                                                                 cAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc                            <  7:57849/149‑1 (MQ=255)
                                                                                                                 cAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc                            <  4:29974/149‑1 (MQ=255)
                                                                                                                   aGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACt                           >  5:113390/1‑148 (MQ=255)
                                                                                                                      ccTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATc                       <  6:113390/149‑1 (MQ=255)
                                                                                                                                ccACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTAccc              >  2:329657/1‑148 (MQ=255)
                                                                                                                                ccACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGCCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCAAATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTAccc              >  7:225049/1‑148 (MQ=255)
                                                                                                                                 cACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAg                                     >  4:159091/1‑124 (MQ=255)
                                                                                                                                 cACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAg                                     <  3:159091/124‑1 (MQ=255)
                                                                                                                                     ccTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGt         >  8:334506/1‑148 (MQ=255)
                                                                                                                                      cTTGCGCAAGGCATCGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGa       >  1:322918/1‑149 (MQ=255)
                                                                                                                                      cTTGCGCAAGGCATCGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGa       >  8:87499/1‑149 (MQ=255)
                                                                                                                                            gcgATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc                            >  7:55614/1‑123 (MQ=255)
                                                                                                                                            gcgATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc                            <  8:55614/123‑1 (MQ=255)
                                                                                                                                            gcgATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCCTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGATCTAt  <  7:225407/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CAGGTTGCCGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCGCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGATCTAT  >  NC_002947/237309‑237596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: