Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,242,672	G→A	8.9%	R82Q (CGG→CAG)	PP_1978 →	TetR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,242,672	0	G	A	8.9%	191.3 / 6.3	67	R82Q (CGG→CAG)	PP_1978	TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base A (2/4);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.74e-01

TGCGGCAGGCCTGACCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTTGAT  >  NC_002947/2242532‑2242816                                                                                                                                             |                                                                                                                                                  tGCGGCAGGCCTGACCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCCCCGGccagcgctcgc                                                                                                                                           >  6:106073/1‑144 (MQ=255) tGCGGCAGGCCTGACCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCCCCGGccagcgctcgc                                                                                                                                           >  6:224331/1‑144 (MQ=255)     gCAGGCCTGACCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGc                                                                                                                                       <  6:70603/149‑1 (MQ=255)      cAGGCCTGACCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGcc                                                                                                                                      >  8:221201/1‑149 (MQ=255)      cAGGCCTGACCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGcc                                                                                                                                      >  8:219251/1‑149 (MQ=255)         gCCTGACCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCggg                                                                                                                                   <  6:116511/149‑1 (MQ=255)         gCCTGACCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCggg                                                                                                                                   <  6:189719/149‑1 (MQ=255)              aCCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑tt                                                                                                                             <  2:215562/149‑1 (MQ=255)              aCCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCCCCGGCCAGCGCTCGCCGCCCGGCGccat                                                                                                                              <  5:224331/149‑3 (MQ=255)              aCCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCCCCGGCCAGCGCTCGCCGCCCGGCGccat                                                                                                                              <  5:106073/149‑3 (MQ=255)                cGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCt                                                                                                                           <  2:108275/149‑1 (MQ=255)                  tCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTAc                                                                                                                         <  5:116053/149‑1 (MQ=255)                      cggcTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACctct                                                                                                                     <  2:303243/149‑1 (MQ=255)                          ttCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTcgcg                                                                                                                 >  5:109846/1‑149 (MQ=255)                          ttCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCCCCGGCCAGCGCTCGCCGCCCGGCGcc                                                                                                                                >  5:104455/1‑135 (MQ=255)                          ttCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCCCCGGCCAGCGCTCGCCGCCCGGCGcc                                                                                                                                <  6:104455/135‑1 (MQ=255)                               tGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAg                                                                                                             >  5:175595/1‑148 (MQ=255)                                    aCTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCgg                                                                                                       >  5:30342/1‑149 (MQ=255)                                      ttCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGAAGGCCCCGGCCAGCGCTCGCCGCCCGGCGCC‑ATcga                                                                                                                          <  5:167376/128‑3 (MQ=25)                                      ttCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGAAGGCCCCGGCCAGCGCTCGCCGCCCGGCGCC‑ATcga                                                                                                                          >  6:167376/1‑126 (MQ=25)                                          aCAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGc                                                                                                 <  2:238532/149‑1 (MQ=255)                                              cAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAg                                                                                             >  3:208441/1‑149 (MQ=255)                                              cAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAg                                                                                             >  7:224438/1‑149 (MQ=255)                                               aaGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGt                                                                                            <  8:224438/149‑1 (MQ=255)                                                 ggACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTa                                                                                           >  5:182962/1‑148 (MQ=255)                                                 ggACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTAc                                                                                          <  7:219251/149‑1 (MQ=255)                                                 ggACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTAc                                                                                          <  7:221201/149‑1 (MQ=255)                                                       gCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCtt                                                                                    <  6:350645/149‑1 (MQ=255)                                                         ttATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATg                                                                                       >  5:294326/1‑144 (MQ=255)                                                         ttATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATg                                                                                       <  6:294326/144‑1 (MQ=255)                                                           aTGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGc                                                                                  <  2:8186/147‑1 (MQ=255)                                                             gCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCgg                                                                                                       >  8:187601/1‑124 (MQ=255)                                                             gCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATg                                                                                       <  2:152895/140‑1 (MQ=255)                                                             gCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATg                                                                                       >  1:152895/1‑140 (MQ=255)                                                                 gAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTc                                                                                                                            >  2:126576/1‑99 (MQ=255)                                                                       ttCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAAcgc                                                                    >  5:352591/1‑149 (MQ=255)                                                                            ccAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTgg                                                               >  4:236370/1‑149 (MQ=255)                                                                             cAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTggg                                                              <  3:64391/149‑1 (MQ=255)                                                                                ttgttgGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTgg                                                               <  1:156618/145‑1 (MQ=255)                                                                                ttgttgGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTgg                                                               >  2:156618/1‑145 (MQ=255)                                                                                  gttgGGTGAGCGCCGTGCCTTGCTCGCCCAGATAGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATTCTTGCCCGCTGCCTAACGCGTTGGGCGaaa                                                         >  7:122464/1‑149 (MQ=255)                                                                                   ttgGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGt                                                                                            >  3:183187/1‑113 (MQ=255)                                                                                   ttgGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGt                                                                                            <  4:183187/113‑1 (MQ=255)                                                                                     gggTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATg                                                       >  6:24018/1‑148 (MQ=255)                                                                                            cgcCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCt                                               <  4:208441/149‑1 (MQ=255)                                                                                                   ccTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGa                                         <  5:24018/148‑1 (MQ=255)                                                                                                    cTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTa                                                                                                                          >  1:346215/1‑66 (MQ=255)                                                                                                    cTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTa                                                                                                                          <  2:346215/66‑1 (MQ=255)                                                                                                    cTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAgg                                       >  6:269925/1‑149 (MQ=255)                                                                                                         tCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAg                                                    <  7:254600/131‑1 (MQ=255)                                                                                                         tCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAg                                                    >  8:254600/1‑131 (MQ=255)                                                                                                         tCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTc                                  >  3:4938/1‑149 (MQ=255)                                                                                                           gCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCcg                                >  5:94529/1‑149 (MQ=255)                                                                                                             ccAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCcgc                               >  4:90491/1‑148 (MQ=255)                                                                                                                gATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAgg                           >  2:43756/1‑149 (MQ=255)                                                                                                                 aTCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGccc                                                                                <  5:181220/95‑1 (MQ=255)                                                                                                                 aTCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGccc                                                                                >  6:181220/1‑95 (MQ=255)                                                                                                                      ccccAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTgg                     <  3:236370/149‑1 (MQ=255)                                                                                                                      ccccAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTgg                     >  4:175180/1‑149 (MQ=255)                                                                                                                      ccccAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTgg                     <  4:4938/149‑1 (MQ=255)                                                                                                                       cccAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTTCCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGa                                         <  1:286914/128‑1 (MQ=255)                                                                                                                       cccAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGa                                         >  2:286914/1‑128 (MQ=255)                                                                                                                         cAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGcc                   >  7:293030/1‑148 (MQ=255)                                                                                                                          aaTCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGa                 <  3:175180/149‑1 (MQ=255)                                                                                                                                gATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGc               <  6:182962/145‑1 (MQ=255)                                                                                                                                     cgcCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGt      <  6:94529/149‑1 (MQ=255)                                                                                                                                     cgcCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGt      <  8:293030/149‑1 (MQ=255)                                                                                                                                      gcCGGGCGGCGAGCGCTGGCCGGGGCCGTTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGt      >  1:192631/1‑149 (MQ=255)                                                                                                                                         gggCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTtgat  <  2:192631/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                  TGCGGCAGGCCTGACCGTCGGCGGCTTCTATGCGCACTTCGACAGCAAGGACGAGCTTATGCTCGAAGCGTTCCGCCAGTTGTTGGGTGAGCGCCGTGCCTTGCTCGCCCAGATCGACCCCAATCTCGATGGCGCCGGGCGGCGAGCGCTGGCCGGGGCC‑TTCTACCTCTCGCGCAAGCACCGGGACTGCGAAGTACATGCTTGCCCGCTGCCTAACGCGTTGGGCGAAATGCAGCGCCTGCCGGAGGTCTTCCGCGAGGTGCTGGCCGAGCACATCGAGTTGAT  >  NC_002947/2242532‑2242816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑