| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 237,455 | C→A | 9.3% | A112A (GCC→GCA) | algR → | alginate biosynthesis regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 237,455 | 0 | C | A | 9.3% | 164.5 / 6.9 | 54 | A112A (GCC→GCA) | algR | alginate biosynthesis regulatory protein |
| Reads supporting (aligned to +/- strand): ref base C (27/22); new base A (3/2); total (30/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01 | |||||||||||
CAGGTTGCCGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGATCTATTTCA > NC_002947/237309‑237600 | cAGGTTGCCGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGACGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCtt > 8:299929/1‑149 (MQ=255) gTTGCCGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTgcg > 6:179414/1‑149 (MQ=255) ccGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCaa < 5:146240/148‑1 (MQ=255) ccGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCAAGGCATCGCGCAAg < 8:102875/149‑1 (MQ=255) ccGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGACGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAg < 7:299929/149‑1 (MQ=255) cGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAgg < 4:2475/149‑1 (MQ=255) tCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCAAGGCATCGCGCAAGGcgt > 5:89034/1‑147 (MQ=255) gCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCAAGGCATCGCGCAAGGcgtgg < 6:89034/149‑5 (MQ=255) gAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCaa > 6:213440/1‑149 (MQ=255) aaCGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAAc < 3:62623/149‑1 (MQ=255) gtgtTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTAc < 5:179414/149‑1 (MQ=255) gtTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTAcc < 2:143468/148‑1 (MQ=255) gtTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTAcc > 1:143468/1‑148 (MQ=255) tttGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACccgg > 7:72083/1‑149 (MQ=255) gatgatGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGa < 1:48401/149‑1 (MQ=255) atgatgAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATg > 7:220043/1‑147 (MQ=255) atgaATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAATCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCaa > 1:265765/1‑106 (MQ=255) atgaATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCaa < 2:265765/106‑1 (MQ=255) tACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAggcggcgg > 5:32027/1‑149 (MQ=255) tACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCCTACCCGGCCGGCCAATGAAggcggcgg > 8:225407/1‑149 (MQ=255) agagGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCACGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCCCg > 8:76779/1‑147 (MQ=255) agagGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGCCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACg > 3:78904/1‑147 (MQ=255) gagGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAg < 8:220043/149‑1 (MQ=255) ggCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGcc < 4:78904/149‑1 (MQ=255) aTTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAAt > 4:8503/1‑124 (MQ=255) aTTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAAt < 3:8503/124‑1 (MQ=255) aTTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCata > 2:349238/1‑149 (MQ=255) aTTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCata > 6:73945/1‑149 (MQ=255) tACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCa < 6:32027/149‑1 (MQ=255) cTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGCTGCGATGCCTTGCGCAAGGCCGAAAAACCCAAc < 1:37909/73‑1 (MQ=255) cTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAAc > 2:37909/1‑72 (MQ=255) tCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCAcgcg > 4:295114/1‑148 (MQ=255) gCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCaaa < 1:349238/149‑1 (MQ=255) tgtgACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAggcggcgg > 3:115277/1‑111 (MQ=255) tgtgACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAggcggcgg < 4:115277/111‑1 (MQ=255) gtgACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCAt > 6:85586/1‑149 (MQ=255) cAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAg > 8:231865/1‑123 (MQ=255) cAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc > 3:29974/1‑149 (MQ=255) cAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc > 8:57849/1‑149 (MQ=255) cAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc < 7:57849/149‑1 (MQ=255) cAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc < 4:29974/149‑1 (MQ=255) aGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACt > 5:113390/1‑148 (MQ=255) ccTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATc < 6:113390/149‑1 (MQ=255) ccACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTAccc > 2:329657/1‑148 (MQ=255) ccACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGCCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCAAATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTAccc > 7:225049/1‑148 (MQ=255) cACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAg < 3:159091/124‑1 (MQ=255) cACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAg > 4:159091/1‑124 (MQ=255) ccTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGt > 8:334506/1‑148 (MQ=255) cTTGC‑GCAAGGCATCGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGa > 1:322918/1‑149 (MQ=255) cTTGC‑GCAAGGCATCGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGa > 8:87499/1‑149 (MQ=255) gcgATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc > 7:55614/1‑123 (MQ=255) gcgATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAAc < 8:55614/123‑1 (MQ=255) gcgATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCCTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGATCTAt < 7:225407/149‑1 (MQ=255) tGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGATCTATTTCa < 5:85586/149‑1 (MQ=255) | CAGGTTGCCGCTCGCCTGTGCGAACGCGAGGCGCCGCCCGCGGTGGTGTTTTGCTCCGGGGATGATGAATACGGTGCAGAGGCATTCAAGGACAGTACCCTCAGCCATGTGACCAAGCCCTTCCAGGCCCACGCCTTGC‑GCGATGCCTTGCGCAAGGCCGAAAAACCCAACCGCGCCCAATTGGCGGCGCTTACCCGGCCGGCCAATGAAGGCGGCGGCCCACGCAGCCATATCAGCGCCCGCACGCGCAAAGGCATCGAACTGATCCCGTTACCCCAGGTGATCTATTTCA > NC_002947/237309‑237600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |