BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,300,416	C→A	6.1%	R314L (CGC→CTC)	sbcD ←	exonuclease subunit SbcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,300,416	0	C	A	6.1%	379.1 / 10.3	115	R314L (CGC→CTC)	sbcD	exonuclease subunit SbcD
Reads supporting (aligned to +/- strand): ref base C (59/49); new base A (5/2); total (64/51)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 115 total aligned reads displayed.

ATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACCGTCCAAC  >  NC_002947/2300271‑2300562

aTCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑Gt                                                                                                                                                  >  8:252854/1‑149 (MQ=255)

      ttCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGt                                                                                                                                            >  6:236289/1‑149 (MQ=255)

          gCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTc                                                                                                                                        <  3:56706/149‑1 (MQ=255)

           cGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTgg                                                                                                                                               <  8:285864/141‑1 (MQ=255)

           cGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTgg                                                                                                                                               >  7:285864/1‑141 (MQ=255)

           cGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCa                                                                                                                                       <  5:43698/149‑1 (MQ=255)

               gttgCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGGACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGc                                                                                                                                    <  3:343399/148‑1 (MQ=255)

                ttgCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCa                                                                                                                                   <  6:284589/148‑1 (MQ=255)

                           gTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAGGCAGG‑TCGACCACgg                                                                                                                      >  6:266043/1‑149 (MQ=255)

                              cTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAGGCAGG‑TCGACCACGGGc                                                                                                                    <  5:266043/148‑1 (MQ=255)

                               tCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAg                                                                                                                  >  6:114952/1‑149 (MQ=255)

                               gtacggcTGATTCGTATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAg                                                                                                                  >  1:94803/6‑149 (MQ=255)

                                cGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCtt                                                                                                                                         <  1:311920/126‑1 (MQ=255)

                                cGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCtt                                                                                                                                         >  2:311920/1‑126 (MQ=255)

                                 ggCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTg                                                                                                                                                >  8:176496/1‑118 (MQ=255)

                                 ggCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTg                                                                                                                                                <  7:176496/118‑1 (MQ=255)

                                     cTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGc                                                                                                            <  5:213572/149‑1 (MQ=255)

                                          aCGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGtt                                                                                                       >  1:300536/1‑149 (MQ=255)

                                           cGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGtt                                                                                                       <  5:236289/148‑1 (MQ=255)

                                            gAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTtgc                                                                                                     >  3:345864/1‑149 (MQ=255)

                                               tCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTtgctgc                                                                                                  <  2:300536/149‑1 (MQ=255)

                                               tCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTtgctg                                                                                                   >  1:16910/1‑148 (MQ=255)

                                                   ccGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCaa                                                                                                >  3:312716/1‑147 (MQ=255)

                                                   ccGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCaa                                                                                                <  4:312716/147‑1 (MQ=255)

                                                     gTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCGTCGAGGGGTGGTAAAGCAGG‑TGGACCACCGGGAGGTCGGCCCGTTGCTGCAACaa                                                                                             >  1:285896/1‑148 (MQ=255)

                                                     gTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGc                                                                                            <  5:114952/149‑1 (MQ=255)

                                                       aCCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCACCAGCTc                                                                                          >  3:189184/1‑149 (MQ=255)

                                                       aCCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTc                                                                                          >  8:229586/1‑149 (MQ=255)

                                                        ccGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTc                                                                                          <  2:135377/148‑1 (MQ=255)

                                                         cGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTtgct                                                                                                    <  5:333896/137‑1 (MQ=255)

                                                         cGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTtgct                                                                                                    >  6:333896/1‑137 (MQ=255)

                                                           ccttgccttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACACGCACCTCCAGACAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGACAGTTGCTGAAACAGCTCgac                                                                                       >  6:225537/1‑146 (MQ=255)

                                                             ttgccttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGcc                                                                                       >  7:23490/1‑146 (MQ=255)

                                                               gccttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCa                                                                                     <  8:23490/146‑1 (MQ=255)

                                                                 cttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGt                                                                                >  2:169595/1‑149 (MQ=255)

                                                                  ttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTgg                                                                                                                                               <  7:276439/86‑1 (MQ=255)

                                                                  ttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTgg                                                                                                                                               >  8:276439/1‑86 (MQ=255)

                                                                  ttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTg                                                                               >  4:173813/1‑149 (MQ=255)

                                                                  ttCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTACAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTg                                                                               >  3:20002/1‑149 (MQ=255)

                                                                   ggcaaggcgGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGc                                                                              <  1:148627/143‑1 (MQ=255)

                                                                       gcgcGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGc                                                                                   <  7:289705/140‑1 (MQ=255)

                                                                       gcgcGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCggg                                                                          >  2:178658/1‑149 (MQ=255)

                                                                        cgcgGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGc                                                                         <  7:252854/149‑1 (MQ=255)

                                                                         gcgGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCg                                                                                  <  4:124792/139‑1 (MQ=255)

                                                                               tCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCg                                                                  >  6:269002/1‑149 (MQ=255)

                                                                                  aTCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTg                                                               >  4:3040/1‑149 (MQ=255)

                                                                                  aTCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTg                                                               >  8:114653/1‑149 (MQ=255)

                                                                                   tCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTg                                                               <  4:28842/148‑1 (MQ=255)

                                                                                   tCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTg                                                               >  3:28842/1‑148 (MQ=255)

                                                                                       ctgGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAg                                                                                                         >  6:319953/1‑102 (MQ=255)

                                                                                       ctgGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAg                                                                                                         <  5:319953/102‑1 (MQ=255)

                                                                                       ctgGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGtt                                                                <  1:169595/143‑1 (MQ=255)

                                                                                               ggTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGgcg                                                  >  3:156251/1‑149 (MQ=255)

                                                                                                  cGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGt                                                                                <  3:46924/116‑1 (MQ=255)

                                                                                                  cGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGt                                                                                >  4:46924/1‑116 (MQ=255)

                                                                                                   gggTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTg                                              <  2:94803/149‑1 (MQ=255)

                                                                                                     gTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTgg                                             >  1:307193/1‑148 (MQ=255)

                                                                                                     gTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTgg                                             >  2:277823/1‑148 (MQ=255)

                                                                                                          ggCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCT‑GTTGGTGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACtgg                                       <  6:339739/149‑1 (MQ=255)

                                                                                                           gCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACgggg                                      >  5:58906/1‑149 (MQ=255)

                                                                                                           gCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACggg                                       >  6:99880/1‑148 (MQ=255)

                                                                                                           gCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACggg                                       <  5:99880/148‑1 (MQ=255)

                                                                                                               gtcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTg                                  >  1:187693/1‑149 (MQ=255)

                                                                                                                tcgAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGGTTGGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTg                                  >  1:270322/1‑149 (MQ=255)

                                                                                                                 cgAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGc                                >  2:309597/1‑149 (MQ=255)

                                                                                                                   aGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGTGTGGCTc                              >  2:47217/1‑149 (MQ=255)

                                                                                                                     cAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTg                                              <  6:131251/131‑1 (MQ=255)

                                                                                                                     cAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTg                                              >  5:131251/1‑131 (MQ=255)

                                                                                                                     cAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGa                            >  5:220328/1‑149 (MQ=255)

                                                                                                                      aaCACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGAc                           <  2:7434/149‑1 (MQ=255)

                                                                                                                       acacCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACg                          <  6:58906/149‑1 (MQ=255)

                                                                                                                          ccccaaCCGCCAGCCCTGGCTGGAG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACggg                                       <  7:182850/128‑1 (MQ=255)

                                                                                                                          ccccaaCCGCCAGCCCTGGCTGGAG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACggg                                       >  8:182850/6‑133 (MQ=255)

                                                                                                                           ccGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGa                      >  4:92035/1‑149 (MQ=255)

                                                                                                                           ccGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGa                      >  2:314027/1‑149 (MQ=255)

                                                                                                                           ccGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGa                      <  5:329908/149‑1 (MQ=255)

                                                                                                                             gCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGccc                    >  6:265771/1‑149 (MQ=255)

                                                                                                                             gCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGAcc                    >  7:300807/1‑149 (MQ=255)

                                                                                                                               aCCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAg                  >  3:28225/1‑149 (MQ=255)

                                                                                                                                 cgccagccctGGCTGGAG‑GTGCGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGAc                           <  8:267208/136‑1 (MQ=255)

                                                                                                                                  tCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGcc               >  4:141676/1‑149 (MQ=255)

                                                                                                                                  tCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGcc               >  2:231131/1‑149 (MQ=255)

                                                                                                                                   ccagccctGGCTGGAG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCg              >  6:272910/1‑149 (MQ=255)

                                                                                                                                   ccagccagGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCg              >  6:352605/1‑149 (MQ=255)

                                                                                                                                     agccagGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGc                         >  1:120720/1‑136 (MQ=255)

                                                                                                                                     agccagGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGc                         <  2:120720/136‑1 (MQ=255)

                                                                                                                                     agccagGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGtt            <  2:187693/149‑1 (MQ=255)

                                                                                                                                      gccagGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTa           <  1:231131/149‑1 (MQ=255)

                                                                                                                                       ccagGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCa                   >  3:318783/1‑140 (MQ=255)

                                                                                                                                       ccagGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCa                   <  4:318783/140‑1 (MQ=255)

                                                                                                                                         ctagCTGGAG‑GTGCGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACCg        >  7:15894/4‑149 (MQ=255)

                                                                                                                                         agGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACCg        >  5:90760/1‑149 (MQ=255)

                                                                                                                                         agGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACCg        <  2:270322/149‑1 (MQ=255)

                                                                                                                                         agGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGAGCCACGCCCCGTACCACGCGCCGTGGcc                               <  6:176094/126‑2 (MQ=255)

                                                                                                                                         agGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGAGCCACGCCCCGTACCACGCGCCGTGGcc                               >  5:176094/1‑125 (MQ=255)

                                                                                                                                           ggCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACCGTc      <  5:269002/149‑1 (MQ=255)

                                                                                                                                            gCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACCGTcc     <  7:199662/149‑1 (MQ=255)

                                                                                                                                             cTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCCCGCGTGGCAGGGCCCCGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCCGGCCGTTACCGTcc     >  7:252202/1‑148 (MQ=255)

                                                                                                                                              tGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACCGTCCaa   >  3:241717/1‑149 (MQ=255)

                                                                                                                                               ggCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACCGTCCAAc  <  3:173813/149‑1 (MQ=255)

ATCGTCTTCGGCGTTGTTGCGGCCGGCGTACTCGGCGCTGATACGAATCAGCCGTACCGCCTTGCCTTCAAGCGCGGTTTCGATCTGCTGGCGCAGGTCGGGTTGCGGCTCGTCGAGCAACACCCGCACCTCCAGCCAGGGCTGGCG‑GTTGGGGTCTTCAAGCAGG‑TCGACCACGGGCAGGTCGGCCAGTTGCTGCAACAGCTCGCCCAGCGGTGCCGGGCCCACCCGTTGCAGGGCCACGGCGCGTGGTACGGGGCGTGGCTCGACGCTGACCAGGCCGTTACCGTCCAAC  >  NC_002947/2300271‑2300562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`