Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,363,386	T→A	6.5%	D164V (GAC→GTC)	PP_2076 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,363,386	0	T	A	6.5%	285.8 / 5.2	97	D164V (GAC→GTC)	PP_2076	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (47/43);  new base A (5/2);  total (52/45)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

TGTGGCGGGGCGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCTGTGCCATTC  >  NC_002947/2363239‑2363534                                                                                                                                                    |                                                                                                                                                       tgtgGCGGGGCGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTc                                                                                                                                                       <  6:126680/149‑1 (MQ=255) tgtgGCGGGGCGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTAGTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTc                                                                                                                                                       <  7:230051/149‑1 (MQ=255)   tgGCGGGGCGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCa                                                                                                                                                      <  2:302622/148‑1 (MQ=255)    ggCGGGGCGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGACAT‑c                                                                                                                                                   >  4:154063/1‑149 (MQ=255)    ggCGGGGCGTAGTAAGGCTTGATGAAGAGGGCGATATCGATTGTGGCCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGAGGGGTCAGCGGGGAAGGGCTGAACAATTGACGCAGCTCCTCGATGTCAta                                                                                                                                                    >  4:214648/1‑148 (MQ=21)       ggggCGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGACAT‑CCAg                                                                                                                                                <  3:154063/149‑1 (MQ=255)              cGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCg                                                                                                                                            >  4:56533/1‑146 (MQ=255)               ggAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑a                                                                                                                                         >  8:31239/1‑147 (MQ=255)                      tGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGAt                                                                                                                                >  4:181782/1‑149 (MQ=255)                        gATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATg                                                                                                                               >  6:33402/1‑148 (MQ=255)                        gATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATg                                                                                                                               <  5:33402/148‑1 (MQ=255)                           gacgCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGGTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGACAT‑CCAGGTCGA‑AGAGCGTGATGGTcc                                                                                                                           >  6:183799/4‑149 (MQ=255)                              gCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑ACAGGGTGATGGTCCAGc                                                                                                                        >  6:72861/1‑149 (MQ=255)                              gCGGGCGATATCGATTGTGGGCAGGCTGCGGCGGTATTCTTCGTCGAGCATGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGTGAAGGGCTGAACAATGGAGGCAGCTCCTGGATGTCAT‑CCAGGTGGT‑AGAGGGTGATGGTCCAGc                                                                                                                        <  3:214648/149‑1 (MQ=21)                                 ggCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGTGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑cc                                                                                                                                                  <  3:112719/121‑1 (MQ=255)                                 ggCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑cc                                                                                                                                                  >  4:112719/1‑121 (MQ=255)                                         cGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCAGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACcgcg                                                                                                              >  1:68846/1‑148 (MQ=255)                                          gATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑aga                                                                                                                                       <  1:311029/122‑1 (MQ=255)                                          gATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑aga                                                                                                                                       >  2:311029/1‑122 (MQ=255)                                             tgtgGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAgg                                                                                                          <  8:35458/148‑1 (MQ=255)                                                    aGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAg                                                                                                                                                <  2:135522/104‑1 (MQ=255)                                                    aGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAg                                                                                                                                                >  1:135522/1‑104 (MQ=255)                                                       ctgctgCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAgg                                                                                                                                     <  4:215577/111‑1 (MQ=255)                                                         gctgcGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGACAT‑CCAggag                                                                                                                                             <  2:297198/102‑3 (MQ=255)                                                         gctgcGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGACAT‑CCAggag                                                                                                                                             >  1:297198/1‑100 (MQ=255)                                                          ctgcGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGAtt                                                                                            <  7:31239/149‑1 (MQ=255)                                                             cGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGcc                                                                                         <  1:66813/149‑1 (MQ=255)                                                              ggTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGAGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCg                                                                                        >  4:62725/1‑149 (MQ=255)                                                                tATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑a                                                                                                                                         >  4:280712/1‑98 (MQ=255)                                                                 aTTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGgcg                                                                                     >  5:162095/1‑149 (MQ=255)                                                                  ttCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGCGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCTT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGgcgc                                                                                    >  1:101948/1‑149 (MQ=255)                                                                     gtcgtcgAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTgg                                                                                 >  4:64043/1‑149 (MQ=255)                                                                       cgtcgAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCa                                                                                                                                                 >  6:106913/1‑84 (MQ=255)                                                                       cgtcgAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCa                                                                                                                                                 <  5:106913/84‑1 (MQ=255)                                                                           gAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAg                                                                           <  3:56533/149‑1 (MQ=255)                                                                           gAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAg                                                                           >  2:249072/1‑149 (MQ=255)                                                                           gAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGAGcct                                                                           <  3:64043/149‑3 (MQ=255)                                                                            aGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACcgcg                                                                                                              >  3:259846/1‑113 (MQ=255)                                                                            aGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACcgcg                                                                                                              <  4:259846/113‑1 (MQ=255)                                                                              cAGGCGGTAGTTGTCGAGCATGCGCTGGGTCTGCGGGGAAGGGCTGAACAATGGACGCACCTCCTGGATGTCAT‑CCAGGTCGT‑AGTGGGTGATGGTCCAGCCGAAACCGCGATTGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCt                                                                        <  2:101948/149‑1 (MQ=25)                                                                               aGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTc                                                                       >  5:331108/1‑149 (MQ=255)                                                                                 gCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCAT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTcc                                                                      >  4:6092/1‑148 (MQ=255)                                                                                 gCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCAT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTcc                                                                      <  3:6092/148‑1 (MQ=255)                                                                                 gCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCGGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGTTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTccc                                                                     <  3:62725/149‑1 (MQ=255)                                                                                   ggTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAg                                                                   >  2:182164/1‑149 (MQ=255)                                                                                    gTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACTATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCg                                                                                        <  8:273669/127‑1 (MQ=255)                                                                                    gTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCg                                                                                        >  7:273669/1‑127 (MQ=255)                                                                                    gTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAgg                                                                  >  6:191476/1‑149 (MQ=255)                                                                                     tAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAggg                                                                 <  6:162095/149‑1 (MQ=255)                                                                                     tAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAggg                                                                 <  5:191476/149‑1 (MQ=255)                                                                                     tAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAggg                                                                 <  6:97701/149‑1 (MQ=255)                                                                                     tAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑ACAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAggg                                                                 <  5:72861/149‑1 (MQ=255)                                                                                       gTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAgggg                                                                <  2:68846/148‑1 (MQ=255)                                                                                       gTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGAGCCTGCCCCCAgcgc                                                                <  3:181782/148‑4 (MQ=25)                                                                                        ttGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAgg                                                                  >  7:115994/1‑145 (MQ=255)                                                                                        ttGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAgg                                                                  <  8:115994/145‑1 (MQ=255)                                                                                           tCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGt                                                            <  6:331108/148‑1 (MQ=255)                                                                                             gAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGAtt                                                                                            <  7:37751/114‑1 (MQ=255)                                                                                             gAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGAtt                                                                                            >  8:37751/1‑114 (MQ=255)                                                                                               gCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCgctg                                                       >  7:103480/1‑149 (MQ=255)                                                                                               gCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCgctg                                                       >  6:177408/1‑149 (MQ=255)                                                                                               gCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCgctg                                                       <  1:249072/149‑1 (MQ=255)                                                                                                cATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCgctgc                                                      >  5:107629/1‑149 (MQ=255)                                                                                                cATGCGCGGGGTCAGCGGGGAAGGGCGGAACAATGCACGCCGCGCCTGGATGTCAT‑CCAGGTCGT‑AGAGCGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCgctgc                                                      <  5:183799/149‑1 (MQ=255)                                                                                                   gcgcGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGa                                                   >  2:208812/1‑149 (MQ=255)                                                                                                      cgGGGTCAGCGCGGAAGGGCGGAAGAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCAACCAGATTCCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGctgctgtaca                                                <  8:105118/149‑5 (MQ=37)                                                                                                           tCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTAcag                                            >  2:119844/1‑148 (MQ=255)                                                                                                              gCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACagcagc                                        <  3:108985/149‑1 (MQ=255)                                                                                                                ggggAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGa                                      <  8:103480/149‑1 (MQ=255)                                                                                                                 gggAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCCCTGATGGTGTAAAGCAGCgcg                                     >  8:274144/1‑147 (MQ=255)                                                                                                                   gAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGt                                                            <  4:218605/124‑1 (MQ=255)                                                                                                                   gAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGt                                                            >  3:218605/1‑124 (MQ=255)                                                                                                                    aaGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCa                                  >  1:280368/1‑149 (MQ=255)                                                                                                                       ggCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTg                               >  6:165942/1‑149 (MQ=255)                                                                                                                       ggCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTg                               <  5:177408/149‑1 (MQ=255)                                                                                                                         cTGAACAATGGACGCAGCTCCTGGATGTCATGCCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTgg                              >  1:37101/1‑149 (MQ=255)                                                                                                                         cTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTggg                                                                                <  4:195326/98‑1 (MQ=255)                                                                                                                         cTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTggg                                                                                >  3:195326/1‑98 (MQ=255)                                                                                                                         cTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTgg                              <  2:37101/148‑1 (MQ=255)                                                                                                                          tGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTgg                              >  8:192081/1‑147 (MQ=255)                                                                                                                                aTGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGTGAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCAc                       >  1:223183/1‑149 (MQ=255)                                                                                                                                aTGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGTGAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCAc                       >  1:218092/1‑149 (MQ=255)                                                                                                                                   gACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCAcgcg                    <  1:208812/148‑1 (MQ=255)                                                                                                                                       cAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACt               <  7:192081/149‑1 (MQ=255)                                                                                                                                           tCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTAcagca                                          >  6:237644/1‑118 (MQ=255)                                                                                                                                           tCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTAcagca                                          <  5:237644/118‑1 (MQ=255)                                                                                                                                           tCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCt           >  4:292001/1‑149 (MQ=255)                                                                                                                                             cTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCtgt         >  2:85045/1‑149 (MQ=255)                                                                                                                                             cTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCtgt         >  8:196690/1‑149 (MQ=255)                                                                                                                                             cTGGATGACAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGAACAGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCtgt         >  1:282952/1‑149 (MQ=255)                                                                                                                                               ggATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCTGTGc       >  2:228826/1‑149 (MQ=255)                                                                                                                                                gATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTAc                <  3:242652/139‑1 (MQ=255)                                                                                                                                                gATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTAc                >  4:242652/1‑139 (MQ=255)                                                                                                                                                gATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGACCCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCTGTGc       >  5:105537/1‑148 (MQ=255)                                                                                                                                                gATGACAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCTGTGc       >  4:274255/1‑148 (MQ=255)                                                                                                                                                   gTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAggggg                                                               <  3:336780/89‑1 (MQ=255)                                                                                                                                                   gTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAggggg                                                               >  4:336780/1‑89 (MQ=255)                                                                                                                                                    tCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCTGTGCCATTc  <  5:165942/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                       TGTGGCGGGGCGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTCAT‑CCAGGTCGT‑AGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCTGTGCCATTC  >  NC_002947/2363239‑2363534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑