Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 70,938 T→C 7.7% T323A (ACC→GCC)  glyS ← glycine‑tRNA ligase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294770,9380TC 7.7% 141.8 / 2.5 52T323A (ACC→GCC) glySglycine‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (27/21);  new base C (2/2);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

GCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAGCAGGCAGAAGTACTTCTGGTTGTCCTGCATG  >  NC_002947/70802‑71080
                                                                                                                                        |                                                                                                                                              
gcggcCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGCCAGGCGCGGgcg                                                                                                                                    >  1:300620/1‑149 (MQ=255)
gcggcCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGCCAGGCGCGGgcg                                                                                                                                    >  1:305728/1‑149 (MQ=255)
      aGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGAc                                                                                                                              >  1:267688/1‑149 (MQ=255)
         cTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACttt                                                                                                                           >  3:44119/1‑149 (MQ=255)
                cacGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTg                                                                                                                    <  3:56675/149‑1 (MQ=255)
                    ttCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCtt                                                                                                                >  3:63548/1‑149 (MQ=255)
                       ggCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGc                                                                                                              <  2:267688/148‑1 (MQ=255)
                        gCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCAc                                                                                                            >  6:330100/1‑149 (MQ=255)
                         ccTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCAc                                                                                                            >  5:217765/1‑148 (MQ=255)
                          cTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGa                                                                                                          <  4:44119/149‑1 (MQ=255)
                             gTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATc                                                                                                        <  1:300231/148‑1 (MQ=255)
                                  acacGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTc                                                                                                  >  1:184254/1‑149 (MQ=255)
                                   cacGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTc                                                                                                  <  6:217765/148‑1 (MQ=255)
                                     cGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCggg                                                                                               <  8:75204/149‑1 (MQ=255)
                                     cGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCggg                                                                                               >  7:75204/1‑149 (MQ=255)
                                        tGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTcg                                                                                            <  7:130885/149‑1 (MQ=255)
                                          ccGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTcgcg                                                                                          <  5:141825/149‑1 (MQ=255)
                                          ccGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGCCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTcgcg                                                                                          <  2:305728/149‑1 (MQ=255)
                                          ccGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGCCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTcgcg                                                                                          <  2:300620/149‑1 (MQ=255)
                                                  aGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCAc                                                                                                                                 >  6:87990/1‑102 (MQ=255)
                                                        gAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCgg                                                                                         <  7:143391/136‑1 (MQ=255)
                                                        gAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCgg                                                                                         >  8:143391/1‑136 (MQ=255)
                                                        gAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGtt                                                                              <  2:184254/147‑1 (MQ=255)
                                                        gAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTgg                                                                            >  5:193554/1‑149 (MQ=255)
                                                              cacGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACgg                                                                      >  8:258309/1‑149 (MQ=255)
                                                               acGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACgg                                                                      >  3:261328/1‑148 (MQ=255)
                                                                       gAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAgc                                                             >  2:135145/1‑149 (MQ=255)
                                                                                gTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTc                                                                                    <  5:346648/117‑1 (MQ=255)
                                                                                gTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTc                                                                                    >  6:346648/1‑117 (MQ=255)
                                                                                 ttGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGcagcag                                                   >  5:272532/1‑149 (MQ=255)
                                                                                    aaGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCGTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCtt                                                <  7:258309/149‑1 (MQ=255)
                                                                                                 gctgctTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTcc                                   <  4:261328/149‑1 (MQ=255)
                                                                                                 gctgctTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTc                                    >  8:239133/1‑148 (MQ=255)
                                                                                                   tgctTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCcag                                 >  3:270628/1‑149 (MQ=255)
                                                                                                   tgctTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCcag                                 >  7:10526/1‑149 (MQ=255)
                                                                                                    gctTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCcag                                 >  6:42243/1‑148 (MQ=255)
                                                                                                     cttcttGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCcagc                                <  1:135145/148‑1 (MQ=255)
                                                                                                       tcttGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAgcagg                             >  1:17992/1‑149 (MQ=255)
                                                                                                        cttGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAgcagg                             >  6:996/1‑148 (MQ=255)
                                                                                                        cttGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAgcagg                             >  4:214110/1‑148 (MQ=255)
                                                                                                            tCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAGCAGGCAGaa                        <  4:63548/149‑1 (MQ=255)
                                                                                                               tGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCATGCACGATCTGCTTCGGGTCCCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTAGCCTTCGCTGTCCACCAGGCAGAAGTa                     >  7:235474/1‑149 (MQ=255)
                                                                                                                 cTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAGCAGGCAGAAGTAc                    <  4:270628/148‑1 (MQ=255)
                                                                                                                 cTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAGCAGGCAGAAGTAc                    <  3:214110/148‑1 (MQ=255)
                                                                                                                     aagaagaaCTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAGCAGGCAGAAGTACTTCTg               >  7:201714/1‑149 (MQ=255)
                                                                                                                       gaagaaCTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTg                                                                             >  7:328668/1‑85 (MQ=255)
                                                                                                                       gaagaaCTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTg                                                                             <  8:328668/85‑1 (MQ=255)
                                                                                                                        aagaaCTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAGCAGGCAGAAGTACTTCTGGtt            >  8:286197/1‑149 (MQ=255)
                                                                                                                         agaaCTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAGCAGGCAGAAGTACTTCTGGtt            <  5:996/148‑1 (MQ=255)
                                                                                                                           aaCTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAGCAGGCAGAAGTACTTCTGGTTGTc         <  7:286197/149‑1 (MQ=255)
                                                                                                                            aCTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGTCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTGCAGCAGGCAGCAGTTCTTCTGGTTGTcc        >  7:248700/1‑149 (MQ=255)
                                                                                                                                  cGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAGCAGGCAGAAGTACTTCTGGTTGTCCTGCAtg  <  2:17992/149‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                              
GCGGCCAGCCTGGAAACACGTTCGGCCTTGTCGTACACGGTGCCGAGCTGAGCCTGGAACACCACGTTCTTGAGGCGCTCGTTGAAGGTTTCCAGTGGCTGCTTCTTGTCCTGCTTGAAGAAGAACTCGGCGTCGGTCAGGCGCGGGCGCACGACTTTTTCGTTGCCTTGCACGATCTGCTTCGGGTCGCGGCTTTCGACGTTGGCCACGGTAATGAAGCGCGGCAGCAGCTTGCCTTCGCTGTCCAGCAGGCAGAAGTACTTCTGGTTGTCCTGCATG  >  NC_002947/70802‑71080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑