Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,454,973 T→A 7.1% Q49L (CAG→CTG)  mfd ← transcription‑repair coupling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,454,9730TA 7.1% 299.9 / 9.9 99Q49L (CAG→CTG) mfdtranscription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base T (40/52);  new base A (4/3);  total (44/55)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 101 total aligned reads displayed.

CAGACGGTACAGGCTGGCGATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTGCGGTAGGCGCAGAACTGACACGG  >  NC_002947/2454825‑2455120
                                                                                                                                                    |                                                                                                                                                   
cAGACGGTACAGGCTGGCGATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCt                                                                                                                                                     >  4:256388/1‑149 (MQ=255)
 aGACGGTACAGGCTGGCGATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTg                                                                                                                                                    <  2:62652/149‑1 (MQ=255)
 aGACGGGACAGGCTGGCGATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGGCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTg                                                                                                                                                    <  1:231076/149‑1 (MQ=255)
    cGGTACAGGCTGGCGATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCt                                                                                                                                                 <  2:191557/149‑1 (MQ=255)
       tACAGGCTGGCGATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGCCTGTc                                                                                                                                              >  3:21257/1‑149 (MQ=255)
                gCGATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTcag                                                                                                                                     <  3:335313/149‑1 (MQ=255)
                gCGATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTca                                                                                                                                      <  4:48500/148‑1 (MQ=255)
                gCGATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTGTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGCGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTc                                                                                                                                       <  2:263271/147‑1 (MQ=255)
                  gATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTcagca                                                                                                                                   <  7:30625/149‑1 (MQ=255)
                       ggtGGGTGATGATGTCCTGTTGCGGCGAGAACAGGTCGTAGGGGAGGTTTTCGCAATCGGGGTACGGCAGCACGGGCAGTTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAgg                                                                                                                              <  4:42436/147‑1 (MQ=255)
                       gcTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAgg                                                                                                                              <  2:76687/149‑1 (MQ=255)
                        cTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTc                                                                                                                                       >  6:72886/1‑139 (MQ=255)
                        cTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTc                                                                                                                                       <  5:72886/139‑1 (MQ=255)
                         tGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAgg                                                                                                                              >  4:338841/1‑147 (MQ=255)
                         tGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAgg                                                                                                                              <  3:338841/147‑1 (MQ=255)
                         tGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGt                                                                                                                            >  1:138524/1‑149 (MQ=255)
                          gggAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGt                                                                                                                            >  1:312463/1‑148 (MQ=255)
                           ggAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTg                                                                                                                           <  6:108751/148‑1 (MQ=255)
                             agaTGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAg                                                                                                                        <  7:233508/149‑1 (MQ=255)
                             agaTGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAg                                                                                                                        <  3:256388/149‑1 (MQ=255)
                              gatgatGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGaa                                                                                                                         >  6:33127/1‑147 (MQ=255)
                              gatgatGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGaa                                                                                                                         <  5:33127/147‑1 (MQ=255)
                                 gatgTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTg                                                                                                                                                    <  2:133032/117‑1 (MQ=255)
                                 gatgTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTg                                                                                                                                                    >  1:133032/1‑117 (MQ=255)
                                 gatgTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTcagca                                                                                                                                   >  8:307201/1‑134 (MQ=255)
                                 gatgTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTcagca                                                                                                                                   <  7:307201/134‑1 (MQ=255)
                                 gatgTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGc                                                                                                                    >  7:299207/1‑149 (MQ=255)
                                    gTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAg                                                                                                                 >  5:37083/1‑149 (MQ=255)
                                     tCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAgc                                                                                                                <  4:49279/149‑1 (MQ=255)
                                          gTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGTGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCgctgc                                                                                                           >  7:6754/1‑149 (MQ=255)
                                                cgAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTcag                                                                                                                                     >  2:326413/1‑117 (MQ=255)
                                                cgAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTcag                                                                                                                                     <  1:326413/117‑1 (MQ=255)
                                                 gagaACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTggcggc                                                                                                    >  3:65364/1‑149 (MQ=255)
                                                 gagaACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCCGCCAGCAGCGCTggccgc                                                                                                    <  8:100046/149‑1 (MQ=255)
                                                     aCAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTcag                                                                                                                                     <  2:34626/112‑1 (MQ=255)
                                                     aCAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTcag                                                                                                                                     >  1:34626/1‑112 (MQ=255)
                                                     aCAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCg                                                                                                >  1:125554/1‑149 (MQ=255)
                                                     aCAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGACTTCg                                                                                                <  2:312463/149‑1 (MQ=255)
                                                      cAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCgg                                                                                               <  4:229350/149‑1 (MQ=255)
                                                         gTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCg                                                                                                                                             >  8:209283/1‑100 (MQ=255)
                                                         gTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCg                                                                                                                                             <  7:209283/100‑1 (MQ=255)
                                                          tCGTAGGGCAGGGTTTCCCAATCGGTGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGAt                                                                                           <  8:6754/149‑1 (MQ=255)
                                                           cGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATg                                                                                          <  1:329631/149‑1 (MQ=255)
                                                               gggCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCCGCCAGCAGCGCTggccgc                                                                                                    <  1:115742/135‑1 (MQ=255)
                                                               gggCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCCGCCAGCAGCGCTggccgc                                                                                                    >  2:115742/1‑135 (MQ=255)
                                                                ggCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCgctgc                                                                                                           >  2:35115/1‑127 (MQ=255)
                                                                ggCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCgctgc                                                                                                           <  1:35115/127‑1 (MQ=255)
                                                                 gCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCg                                                                                                                      <  4:246194/115‑1 (MQ=255)
                                                                     ggTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGa                                                                                <  2:38325/149‑1 (MQ=255)
                                                                     ggTGTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGa                                                                                <  6:232790/149‑1 (MQ=255)
                                                                      gTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTggc                                                                                                       <  3:196056/125‑1 (MQ=255)
                                                                      gTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTggc                                                                                                       >  4:196056/1‑125 (MQ=255)
                                                                          cccAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATg                                                                                          >  3:202998/1‑134 (MQ=255)
                                                                          cccAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATg                                                                                          <  4:202998/134‑1 (MQ=255)
                                                                          cccAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGggc                                                                            <  6:37083/148‑1 (MQ=255)
                                                                           ccAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGggcgg                                                                          <  4:285103/149‑1 (MQ=255)
                                                                           ccAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGCTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGggcgg                                                                          >  8:3936/1‑149 (MQ=255)
                                                                             aaTCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATgg                                                                                         <  7:306191/132‑1 (MQ=255)
                                                                             aaTCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATgg                                                                                         >  8:306191/1‑132 (MQ=255)
                                                                                 ggggAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCg                                                                     <  8:299207/148‑1 (MQ=255)
                                                                                  gggAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCggg                                                                   >  3:320122/1‑149 (MQ=255)
                                                                                   ggAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCggg                                                                   >  5:230046/1‑148 (MQ=255)
                                                                                         gcagcaCGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCgg                                                                    <  5:72738/141‑1 (MQ=255)
                                                                                         gcagcaCGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCgg                                                                    >  6:72738/1‑141 (MQ=255)
                                                                                          cagcaCGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGc                                                           >  5:245196/1‑149 (MQ=255)
                                                                                           agcaCGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGcc                                                          <  2:125554/149‑1 (MQ=255)
                                                                                             caCGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGccc                                                         >  1:151573/1‑148 (MQ=255)
                                                                                                   cAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGgttt                                                  <  1:260542/149‑1 (MQ=255)
                                                                                                   cAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGgttt                                                  <  6:245196/149‑1 (MQ=255)
                                                                                                    aGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGgtttg                                                 <  6:230046/149‑1 (MQ=255)
                                                                                                      gTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGgtttgtt                                               <  4:320122/149‑1 (MQ=255)
                                                                                                      gTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGgtttgtt                                               >  7:89176/1‑149 (MQ=255)
                                                                                                         tGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTgc                                            >  6:115727/1‑149 (MQ=255)
                                                                                                           gggCAAAGAAGCCCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCTGCACCGGGCAGGTTGCCCCAGGtttgtttg                                             <  4:65364/146‑1 (MQ=255)
                                                                                                             gCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTgccggc                                        >  3:156354/1‑149 (MQ=255)
                                                                                                             gCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTgccggc                                        >  2:88002/1‑149 (MQ=255)
                                                                                                                aagaagCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGCGGGCGGCACCGGGCAGGTTGCCCCAGGTGTGTTTGACGTCCGt                                     >  4:267875/1‑149 (MQ=255)
                                                                                                                 agaagCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCAGTg                                    >  8:314153/1‑149 (MQ=255)
                                                                                                                    cgcgcAGCTCCTGCTGCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGcc                                 <  4:21257/148‑1 (MQ=255)
                                                                                                                    agCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGcc                                 >  8:58261/1‑149 (MQ=255)
                                                                                                                        cAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACa                             <  8:89176/149‑1 (MQ=255)
                                                                                                                           cTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTgccg                                          <  1:292359/133‑1 (MQ=255)
                                                                                                                           cTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTgccg                                          >  2:292359/1‑133 (MQ=255)
                                                                                                                            tCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTg                                                                                 >  7:99931/1‑93 (MQ=255)
                                                                                                                            tCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTg                         >  8:55954/1‑149 (MQ=255)
                                                                                                                            cccTGCTGCTGACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTg                         >  6:335939/2‑149 (MQ=25)
                                                                                                                            cccTGCTGCTGACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTg                         >  6:71013/2‑149 (MQ=25)
                                                                                                                             ccTGCTGCTGACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTGc                        >  3:54745/1‑149 (MQ=37)
                                                                                                                             ccTGCTGCTGACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTGc                        >  6:71628/1‑149 (MQ=37)
                                                                                                                              ctgctgctgACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTGCg                       <  5:71628/149‑1 (MQ=37)
                                                                                                                                 cTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAACCGCCAGCAGCGCTggc                                                                                                       <  5:75877/66‑1 (MQ=255)
                                                                                                                                 cTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAACCGCCAGCAGCGCTggc                                                                                                       >  6:75877/1‑66 (MQ=255)
                                                                                                                                 cTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGACAGCAGCAGGGTGAAGCCGCCAGCGCTCCTGGCGGCTTCGGCCATGGCAAGCCTGAGGGCCCCACCTGGCAGGTTGCCCCAGCTTAGTTTGCCGGCCCTGTCCGACATTAGCCGTa                    >  7:302447/1‑149 (MQ=255)
                                                                                                                                  tCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTCGCGGTAg                   >  6:319079/1‑149 (MQ=255)
                                                                                                                                    ctgACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTGCGGTAGgc                 <  4:54745/147‑1 (MQ=38)
                                                                                                                                    cAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTGCGGTAGgc                 <  7:58261/149‑1 (MQ=255)
                                                                                                                                          gTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTCGCGGTAGGCGCAGaa           <  5:319079/149‑1 (MQ=255)
                                                                                                                                           ccgacagcCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTGCGGTAGGCGCAGAAc          <  5:335939/143‑1 (MQ=255)
                                                                                                                                            cggcggCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTGCGGTAGGCGCAGAACt         <  5:115727/149‑1 (MQ=255)
                                                                                                                                                    tGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTGCGGTAGGCGCAGAACTGACACgg  >  2:72280/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGACGGTACAGGCTGGCGATGCGCTGGGAGATGATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGTGGCCGACATTTGCGGTAGGCGCAGAACTGACACGG  >  NC_002947/2454825‑2455120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑