Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,455,004 G→C 7.7% R39G (CGC→GGC)  mfd ← transcription‑repair coupling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,455,0040GC 7.7% 275.9 / 9.3 89R39G (CGC→GGC) mfdtranscription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base G (40/42);  new base C (4/3);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

GATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTCCAAGCGTTGCGGCAAAGA  >  NC_002947/2454858‑2455150
                                                                                                                                                  |                                                                                                                                                   
gatgTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGc                                                                                                                                                   >  7:299207/1‑149 (MQ=255)
   gTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAg                                                                                                                                                >  5:37083/1‑149 (MQ=255)
    tCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAgc                                                                                                                                               <  4:49279/149‑1 (MQ=255)
         gTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGTGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCgctgc                                                                                                                                          >  7:6754/1‑149 (MQ=255)
                gagaACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTggcggc                                                                                                                                   >  3:65364/1‑149 (MQ=255)
                gagaACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCCGCCAGCAGCGCTggccgc                                                                                                                                   <  8:100046/149‑1 (MQ=255)
                    aCAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCg                                                                                                                               >  1:125554/1‑149 (MQ=255)
                    aCAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGACTTCg                                                                                                                               <  2:312463/149‑1 (MQ=255)
                     cAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCgg                                                                                                                              <  4:229350/149‑1 (MQ=255)
                         tCGTAGGGCAGGGTTTCCCAATCGGTGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGAt                                                                                                                          <  8:6754/149‑1 (MQ=255)
                          cGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATg                                                                                                                         <  1:329631/149‑1 (MQ=255)
                              gggCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCCGCCAGCAGCGCTggccgc                                                                                                                                   >  2:115742/1‑135 (MQ=255)
                              gggCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCCGCCAGCAGCGCTggccgc                                                                                                                                   <  1:115742/135‑1 (MQ=255)
                               ggCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCgctgc                                                                                                                                          <  1:35115/127‑1 (MQ=255)
                               ggCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCgctgc                                                                                                                                          >  2:35115/1‑127 (MQ=255)
                                gCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCg                                                                                                                                                     <  4:246194/115‑1 (MQ=255)
                                    ggTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGa                                                                                                               <  2:38325/149‑1 (MQ=255)
                                    ggTGTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGa                                                                                                               <  6:232790/149‑1 (MQ=255)
                                     gTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTggc                                                                                                                                      <  3:196056/125‑1 (MQ=255)
                                     gTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTggc                                                                                                                                      >  4:196056/1‑125 (MQ=255)
                                         cccAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATg                                                                                                                         >  3:202998/1‑134 (MQ=255)
                                         cccAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATg                                                                                                                         <  4:202998/134‑1 (MQ=255)
                                         cccAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGggc                                                                                                           <  6:37083/148‑1 (MQ=255)
                                          ccAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGggcgg                                                                                                         <  4:285103/149‑1 (MQ=255)
                                          ccAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGCTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGggcgg                                                                                                         >  8:3936/1‑149 (MQ=255)
                                            aaTCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATgg                                                                                                                        <  7:306191/132‑1 (MQ=255)
                                            aaTCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATgg                                                                                                                        >  8:306191/1‑132 (MQ=255)
                                                ggggAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCg                                                                                                    <  8:299207/148‑1 (MQ=255)
                                                 gggAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCggg                                                                                                  >  3:320122/1‑149 (MQ=255)
                                                  ggAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCggg                                                                                                  >  5:230046/1‑148 (MQ=255)
                                                        gcagcaCGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCgg                                                                                                   <  5:72738/141‑1 (MQ=255)
                                                        gcagcaCGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCgg                                                                                                   >  6:72738/1‑141 (MQ=255)
                                                         cagcaCGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGc                                                                                          >  5:245196/1‑149 (MQ=255)
                                                          agcaCGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGcc                                                                                         <  2:125554/149‑1 (MQ=255)
                                                            caCGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGccc                                                                                        >  1:151573/1‑148 (MQ=255)
                                                                  cAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGgttt                                                                                 <  1:260542/149‑1 (MQ=255)
                                                                  cAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGgttt                                                                                 <  6:245196/149‑1 (MQ=255)
                                                                   aGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGgtttg                                                                                <  6:230046/149‑1 (MQ=255)
                                                                     gTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGgtttgtt                                                                              >  7:89176/1‑149 (MQ=255)
                                                                     gTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGgtttgtt                                                                              <  4:320122/149‑1 (MQ=255)
                                                                        tGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTgc                                                                           >  6:115727/1‑149 (MQ=255)
                                                                          gggCAAAGAAGCCCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCTGCACCGGGCAGGTTGCCCCAGGtttgtttg                                                                            <  4:65364/146‑1 (MQ=255)
                                                                            gCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTgccggc                                                                       >  3:156354/1‑149 (MQ=255)
                                                                            gCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTgccggc                                                                       >  2:88002/1‑149 (MQ=255)
                                                                               aagaagCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGCGGGCGGCACCGGGCAGGTTGCCCCAGGTGTGTTTGACGTCCG‑t                                                                   >  4:267875/1‑149 (MQ=255)
                                                                                agaagCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCAG‑Tg                                                                  >  8:314153/1‑149 (MQ=255)
                                                                                   cgcgcAGCTCCTGCTGCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGcc                                                               <  4:21257/148‑1 (MQ=255)
                                                                                   agCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGcc                                                               >  8:58261/1‑149 (MQ=255)
                                                                                       cAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACa                                                           <  8:89176/149‑1 (MQ=255)
                                                                                          cTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTgccg                                                                         <  1:292359/133‑1 (MQ=255)
                                                                                          cTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTgccg                                                                         >  2:292359/1‑133 (MQ=255)
                                                                                           tCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTg                                                                                                                <  8:99931/93‑1 (MQ=255)
                                                                                           tCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTg                                                                                                                >  7:99931/1‑93 (MQ=255)
                                                                                           tCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTg                                                       >  8:55954/1‑149 (MQ=255)
                                                                                           cccTGCTGCTGACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTg                                                       >  6:335939/2‑149 (MQ=25)
                                                                                           cccTGCTGCTGACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTg                                                       >  6:71013/2‑149 (MQ=25)
                                                                                            ccTGCTGCTGACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGc                                                      >  3:54745/1‑149 (MQ=37)
                                                                                            ccTGCTGCTGACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGc                                                      >  6:71628/1‑149 (MQ=37)
                                                                                             ctgctgctgACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCg                                                     <  5:71628/149‑1 (MQ=37)
                                                                                                cTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAACCGCCAGCAGCGCTggc                                                                                                                                      <  5:75877/66‑1 (MQ=255)
                                                                                                cTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAACCGCCAGCAGCGCTggc                                                                                                                                      >  6:75877/1‑66 (MQ=255)
                                                                                                cTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGACAGCAGCAGGGTGAAGCCGCCAGCGCTCCTGGCGGCTTCGGCCATGGCAAGCCTGAGGGCCCCACCTGGCAGGTTGCCCCAGCTTAGTTTGCCGGCCC‑TGTCCGACATTAGCCGTa                                                  >  7:302447/1‑149 (MQ=255)
                                                                                                 tCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTCGCGGTAg                                                 >  6:319079/1‑149 (MQ=255)
                                                                                                   ctgACGGCCGACAGCCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGgc                                               <  4:54745/147‑1 (MQ=38)
                                                                                                   cAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGgc                                               <  7:58261/149‑1 (MQ=255)
                                                                                                         gTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTCGCGGTAGGCGCAGaa                                         <  5:319079/149‑1 (MQ=255)
                                                                                                          ccgacagcCAGGCCGCCGACCGTCTGGAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAAc                                        <  5:335939/143‑1 (MQ=255)
                                                                                                           cggcggCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACt                                       <  5:115727/149‑1 (MQ=255)
                                                                                                                   tGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACgg                                >  2:72280/1‑148 (MQ=255)
                                                                                                                     gCTGTCGGCCGTCAGCACCACGCTGAAGCGGCCAGCGCTGCTGGCGGCTACGGCGATGGCAAGGCTGAGGGCGGCACCCGGCAGGTTGCCCCACGTTTGTTTGCCGCCCG‑TGGCCGACATTTGCGGTAGCCGCAGAACTGACACgg                                >  4:321893/1‑146 (MQ=255)
                                                                                                                       tGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGA‑GGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGAtt                          >  4:303931/1‑149 (MQ=255)
                                                                                                                        gTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGAtt                          >  4:40361/1‑149 (MQ=255)
                                                                                                                         tCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTg                         <  7:55954/149‑1 (MQ=255)
                                                                                                                           ggCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCGATGGCCGACATTTGCGGTAGGCGCAGaa                                         <  1:9887/132‑1 (MQ=255)
                                                                                                                           ggCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGaa                                         >  2:9887/1‑131 (MQ=255)
                                                                                                                           ggCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGc                        <  3:267875/148‑1 (MQ=255)
                                                                                                                           ggCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCa                       <  2:151573/149‑1 (MQ=255)
                                                                                                                            gCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGccc                      >  8:259752/1‑147 (MQ=255)
                                                                                                                               gTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCGGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTc                    >  8:152778/1‑148 (MQ=255)
                                                                                                                               gTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTc                    >  1:288127/1‑148 (MQ=255)
                                                                                                                                tCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCg                                                     <  4:20109/114‑1 (MQ=255)
                                                                                                                                tCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCg                                                     >  3:20109/1‑114 (MQ=255)
                                                                                                                                     agcagGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGA‑GGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTCCAAGCGtt            <  3:303931/149‑1 (MQ=255)
                                                                                                                                       cagGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGGGGTAGGCGCAGAACTGACACGGGAGATTGCACTCCAAGCGTTg           <  7:3936/149‑1 (MQ=255)
                                                                                                                                       cagGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTCCAAGCGTTg           >  1:220760/1‑149 (MQ=255)
                                                                                                                                        agGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTCCAAGCGTTGc          <  1:72280/149‑1 (MQ=255)
                                                                                                                                         gggTGAAGCGGGCAGGGCTGCTGGCGGCTTCGGCGATGGCAAGGATGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTCCAAGCGTTGc          <  8:302447/148‑1 (MQ=255)
                                                                                                                                          gccgAAGCCGCCAGCAGCGCTGGCCGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTCCAAGCGTTGCgg        >  5:96873/4‑149 (MQ=255)
                                                                                                                                              aaGCGGCCAGCGCTGCTGGCGGCTTCGGCTATGGCAAGGCTGAGGGCGGCACCGGTCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGGAGAACTGACACGGGAGATTGCACTCCAAGCGTTGCGGCaaa    <  3:321893/149‑1 (MQ=255)
                                                                                                                                               agtggCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGGCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTCCAAGCGTTGCGGCa      >  4:10937/4‑146 (MQ=255)
                                                                                                                                                gCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTCCAAGCGTTGCGGCAAaga  <  1:88002/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GATGTCCTGGTGCGGCGAGAACAGGTCGTAGGGCAGGGTTTCCCAATCGGGGAACGGCAGCACGGGCAGGTCTGGGGCAAAGAAGCGCAGCTCCTGCTCCAGACGGTCGGCGGCCTGGCTGTCGGCCGTCAGCAGCAGGGTGAAGCGGCCAGCGCTGCTGGCGGCTTCGGCGATGGCAAGGCTGAGGGCGGCACCGGGCAGGTTGCCCCAGGTTTGTTTGCCGGCCG‑TGGCCGACATTTGCGGTAGGCGCAGAACTGACACGGGAGATTGCACTCCAAGCGTTGCGGCAAAGA  >  NC_002947/2454858‑2455150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑