Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,564,324 C→T 9.1% G141G (GGC→GGT)  PP_2248 → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,564,3240CT 9.1% 118.2 / 2.7 45G141G (GGC→GGT) PP_2248putative transporter
Reads supporting (aligned to +/- strand):  ref base C (18/22);  new base T (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

GCCGTCACCGTGGTCATGCTGGCTGGCGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCATTGGCTGCTGGGTGCGTTCTTGT  >  NC_002947/2564178‑2564469
                                                                                                                                                  |                                                                                                                                                 
gCCGTCACCGTGGTCATGCTGGCTGGCGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGgcgc                                                                                                                                                 >  3:178304/1‑149 (MQ=255)
  cGTCACCGTGGTCATGCTGGCTGGCGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCa                                                                                                                                               >  5:245876/1‑149 (MQ=255)
   gTCACCGTGGTCATGCTGGCTGGCGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTTGACGTGCGCAAGATGATGCTTGCAATTGGCGCCAt                                                                                                                                              <  7:265450/149‑1 (MQ=255)
      aCCGTGGTCATGCTGGCTGGCGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATca                                                                                                                                            <  6:137981/148‑1 (MQ=255)
       ccGTGGTCATGCTGGCTGGCGGTGGAGCATTGCTGGAGAAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATcaca                                                                                                                                          >  3:150704/1‑149 (MQ=255)
               aTGCTGGCTGGCGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGc                                                                                                                                   <  4:93667/148‑1 (MQ=255)
                 gctggctgGCGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGa                                                                                                                                >  6:302199/1‑149 (MQ=255)
                  ctggctggCGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGTGCCATCACACCCTTGCTGAt                                                                                                                               >  5:337897/1‑149 (MQ=255)
                      ctggcGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCGGGACGTGCGCAAGATGAGGCTTGCAATTGGCGCCATCACACCCTTGCTGATccgg                                                                                                                           <  1:243685/149‑3 (MQ=255)
                         gcGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCtggtg                                                                                                                        <  5:302199/149‑1 (MQ=255)
                         gcGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCtggt                                                                                                                         <  6:245876/148‑1 (MQ=255)
                         gcGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCtggt                                                                                                                         <  4:178304/148‑1 (MQ=255)
                                      gCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTTGTGACGCTGGTGGTTGTCTGTATCGTTTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATa                                                                                                            <  2:50416/148‑1 (MQ=255)
                                      gCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGTGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATa                                                                                                            <  6:337897/148‑1 (MQ=255)
                                                       gCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGt                                                                                                     >  4:127459/1‑138 (MQ=255)
                                                       gCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGt                                                                                                     <  3:127459/138‑1 (MQ=255)
                                                       gCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGt                                                                                              <  7:272776/145‑1 (MQ=255)
                                                       gCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGt                                                                                              >  8:272776/1‑145 (MQ=255)
                                                            ccGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCcg                                                                                     >  6:347625/1‑149 (MQ=255)
                                                             cGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCAt                                                                                                                                              <  8:332650/91‑1 (MQ=255)
                                                             cGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCAt                                                                                                                                              >  7:332650/1‑91 (MQ=255)
                                                                      ccGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACGCTTGCTGATCCTGGTGGCTACGGCGATAGCGCTGTATGCCGTGATCACCCGagagcagagc                                                                           >  5:120894/1‑149 (MQ=255)
                                                                          caccggccTGGCGACGCTGCTGGCCGTCGGTATCGTCAGCCTGGACGTCCGCACGACCATCCTTGCAATTGGCGCCATCACACCCTTGCTGACCCTGGTCGCTTCGCCGATACCGCTGTATGCCGCCATCACCCCCGAGCAGAGCTTtg                                                                       <  6:120894/142‑1 (MQ=255)
                                                                                         gCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGcc                                                                                                <  7:208083/109‑1 (MQ=255)
                                                                                         gCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGcc                                                                                                >  8:208083/1‑109 (MQ=255)
                                                                                           tggtggtggTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTg                                                      >  4:128014/1‑149 (MQ=255)
                                                                                             gtggtggtCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGTGCCAt                                                                                                                                              <  4:221919/59‑1 (MQ=255)
                                                                                             gtggtggtCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGTGCCAt                                                                                                                                              >  3:221919/1‑59 (MQ=255)
                                                                                                   gtCGGTATCGTGTGCCTGCACGTGCGCAAGATGATGCTTGCAATTGGAGCCATACCCCCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTCTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGgccagc                                                >  4:21173/1‑147 (MQ=255)
                                                                                                              gtgCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGgcc                                                   <  4:226549/133‑1 (MQ=255)
                                                                                                              gtgCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGgcc                                                   >  3:226549/1‑133 (MQ=255)
                                                                                                                           cgcAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCATTg                      >  3:340571/1‑149 (MQ=255)
                                                                                                                                 atgatgCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCATTGgct                   <  4:264943/146‑1 (MQ=255)
                                                                                                                                 atgatgCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACACGCCATTGgct                   >  3:264943/1‑146 (MQ=255)
                                                                                                                                  tgatgCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCATTGGctgctg               >  3:275024/1‑149 (MQ=255)
                                                                                                                                        ttGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTtgct                                                                     <  8:218078/89‑1 (MQ=255)
                                                                                                                                        ttGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTtgct                                                                     >  7:218078/1‑89 (MQ=255)
                                                                                                                                        ttGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAAc                                         <  6:262529/117‑1 (MQ=255)
                                                                                                                                        ttGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAAc                                         >  5:262529/1‑117 (MQ=255)
                                                                                                                                        ttGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCATTGGCTGCTGGGTGCg         >  2:271062/1‑149 (MQ=255)
                                                                                                                                             aTTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCATTGGCTGCTGGGTGCGTTCtt    <  1:271062/149‑1 (MQ=255)
                                                                                                                                             aTTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCATTGGCTGCTGGGTGCGTTCtt    <  4:340571/149‑1 (MQ=255)
                                                                                                                                                ggCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCAtt                       >  6:188338/1‑127 (MQ=255)
                                                                                                                                                ggCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCAtt                       <  5:188338/127‑1 (MQ=255)
                                                                                                                                                ggCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCATTGGCTGCTGGGTGCGTTCTTGt  <  4:275024/148‑1 (MQ=255)
                                                                                                                                                ggCGCCATCACACCCTTGCTGATCCTGGGGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTGTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCATTGGCTGCTGGGTGCGTTCTTGt  <  5:347625/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCCGTCACCGTGGTCATGCTGGCTGGCGGTGGAGCATTGCTGGAGCAGCAGTTCGGCATCCCGGCCTTGACCGGCAGCGTGCTGGTGACGCTGGTGGTGGTCGGTATCGTGTGCCTGGACGTGCGCAAGATGATGCTTGCAATTGGCGCCATCACACCCTTGCTGATCCTGGTGGCTTCGGCGATAGCGCTGTATGCCGTGATCACCCGCGAGCAGAGCTTTGCTGCGCTCGATCAGGTGGCCAGCCAGCAACAGGCCGGCACCCGCCATTGGCTGCTGGGTGCGTTCTTGT  >  NC_002947/2564178‑2564469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑