Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,573,556 A→G 10.0% E104E (GAA→GAG)  PP_2257 → aerotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,573,5560AG10.0% 127.6 / 5.6 50E104E (GAA→GAG) PP_2257aerotaxis receptor
Reads supporting (aligned to +/- strand):  ref base A (24/21);  new base G (3/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

GTGCGCCACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTGCTGGCGCAGGCC  >  NC_002947/2573410‑2573700
                                                                                                                                                  |                                                                                                                                                
gTGCGCCACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAg                                                                                                                                                 <  6:102037/148‑1 (MQ=255)
     ccACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGc                                                                                                                                           >  2:85770/1‑149 (MQ=255)
       aCCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCgg                                                                                                                                         <  1:85770/149‑1 (MQ=255)
                            tgtACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTg                                                                                                                    >  4:202531/1‑149 (MQ=255)
                                cGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTgcgc                                                                                                                 <  4:152909/148‑1 (MQ=255)
                                cGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTgcgc                                                                                                                 <  6:55256/148‑1 (MQ=255)
                                 gAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGcgcg                                                                                                                >  6:235114/1‑148 (MQ=255)
                                   gTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTAt                                                                                                             >  5:284692/1‑149 (MQ=255)
                                         gtgGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTa                                                                                                       >  8:159202/1‑149 (MQ=255)
                                          tgGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTAc                                                                                                      >  1:347923/1‑149 (MQ=255)
                                                  ccTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGa                                                                                                <  5:2277/147‑1 (MQ=255)
                                                  acTGAAAGCCGGCAAGAGCTGGATGGGTATCGACAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCAACGTCACGCCCATCCTCGCAGGTGGGCGGGGAGTGGGTTACAAGTCGGTGCGCGTATGCACTACGCGCGAg                                                                                               >  6:127600/2‑148 (MQ=255)
                                                    tGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCgg                                                                                                                                         <  2:316717/104‑1 (MQ=255)
                                                    tGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCgg                                                                                                                                         >  1:316717/1‑104 (MQ=255)
                                                     gAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAg                                                                                           >  7:47700/1‑149 (MQ=255)
                                                      aacgCCGGCAAGAGCTGGTTGGGTATCGTCAAGAACGGCTGCCGTAACTGCGACTACTACTTGGTCAATGCCTTCGTCACGCCCATCCTCGAATGGGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGATCaa                                                                                            <  5:127600/144‑1 (MQ=255)
                                                      aaaGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCaa                                                                                            <  3:202531/147‑1 (MQ=255)
                                                      aaaGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAg                                                                                           <  6:284692/148‑1 (MQ=255)
                                                       aaGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTa                                                                                         <  8:47700/149‑1 (MQ=255)
                                                          ccGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGcc                                                                                      >  1:254961/1‑149 (MQ=255)
                                                            ggCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCg                                                                                    >  4:324674/1‑149 (MQ=255)
                                                                      ggATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGt                                                                                          <  3:74987/133‑1 (MQ=255)
                                                                      ggATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGt                                                                                          >  4:74987/1‑133 (MQ=255)
                                                                       gATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGct                                                                          <  2:254961/148‑2 (MQ=255)
                                                                        aTGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCt                                                                        <  5:235114/149‑1 (MQ=255)
                                                                                 gTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGcgcg                                                               >  4:161080/1‑149 (MQ=255)
                                                                                  tCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTActa                                                                                                                           <  4:198585/88‑3 (MQ=255)
                                                                                  tCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGa                                                                                                                           >  3:198585/1‑88 (MQ=255)
                                                                                           gCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAAc                                                     <  5:341253/149‑1 (MQ=255)
                                                                                            cTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACg                                                    <  7:159202/149‑1 (MQ=255)
                                                                                            cTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACg                                                    >  7:24547/1‑149 (MQ=255)
                                                                                            cTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCAGCCGCGCata                                                     >  1:147315/1‑146 (MQ=255)
                                                                                               ccGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCAGCCGCGCataca                                                   <  2:147315/147‑5 (MQ=255)
                                                                                                            tactactGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTc                                    >  8:48679/1‑149 (MQ=255)
                                                                                                              ctactGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTc                                    <  3:161080/147‑1 (MQ=255)
                                                                                                                         aTGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAACAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCgg                       >  2:251299/1‑149 (MQ=255)
                                                                                                                             cTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCt                     >  5:165071/1‑147 (MQ=255)
                                                                                                                                  acccGCCCACCTTCGAGGATGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTg              >  7:328646/4‑149 (MQ=255)
                                                                                                                                    ccGCCCACCTTCGAGGATGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGgcgccgc                            >  2:58628/2‑133 (MQ=255)
                                                                                                                                    ccGCCCACCTTCGAGGATGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGgcgccgc                            <  1:58628/132‑1 (MQ=255)
                                                                                                                                     cGCCCACCTTCGAGGATGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGa                   >  8:47764/1‑141 (MQ=255)
                                                                                                                                     cGCCCACCTTCGAGGATGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGa                   <  7:47764/141‑1 (MQ=255)
                                                                                                                                          aTCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTg              >  2:242877/1‑141 (MQ=255)
                                                                                                                                          aTCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTg              <  1:242877/141‑1 (MQ=255)
                                                                                                                                              tCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGa                   <  2:215157/132‑1 (MQ=255)
                                                                                                                                              tCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTGCTGgc         >  2:110664/1‑142 (MQ=255)
                                                                                                                                              tCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTGCTGgc         <  1:110664/142‑1 (MQ=255)
                                                                                                                                              tCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTGCTGGCGCAggcc  >  3:34234/1‑149 (MQ=255)
                                                                                                                                              tCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTGCTGGCGCAggcc  >  5:115121/1‑149 (MQ=255)
                                                                                                                                              tCGAAGGTGGGCGGGTAGTGGGTTACCAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGa                   >  1:215157/1‑132 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GTGCGCCACCCGGACATGCCGCCGGCGGTGTACGAGTTGATGTGGCGCTACCTGAAAGCCGGCAAGAGCTGGATGGGTATCGTCAAGAACCGCTGCCGTAACGGCGACTACTACTGGGTCAATGCCTACGTCACGCCCATCCTCGAAGGTGGGCGGGTAGTGGGTTACGAGTCGGTGCGCGTATGCCCTACGCGCGAGCAAGTAGCCCGTGCCGAAGCCCTGTATGCGCGGCTGCGCAACGGCCAGGCGGCATTGTCGGCGCCGCGCCGGCTGAGCTTGCTGGCGCAGGCC  >  NC_002947/2573410‑2573700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑