Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,577,533 G→A 6.9% H288Y (CAC→TAC)  PP_2259 ← sigma‑54 dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,577,5330GA 6.9% 171.9 / 2.1 58H288Y (CAC→TAC) PP_2259sigma‑54 dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base A (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

AACACTTCTTTGCCTACGCCTGTTTCGCCCTCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGG  >  NC_002947/2577388‑2577681
                                                                                                                                                 |                                                                                                                                                    
aaCACTTCTTTGCCTACGCCTGTTTCGCCCTCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCGTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCa                                                                                                                                                   <  3:190410/149‑1 (MQ=255)
    cttcttTGCCTACGCCTGTTTCGCCCTCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTgg                                                                                                                                               <  1:175187/149‑1 (MQ=255)
            ccTACGCCTGTTTCGCCCTCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGt                                                                                                                                           <  2:59940/145‑1 (MQ=255)
            ccTACGCCTGTTTCGCCCTCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGt                                                                                                                                           >  1:59940/1‑145 (MQ=255)
            ccTACGCCTGTTTCGCCCTCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGaaa                                                                                                                                       <  3:228518/149‑1 (MQ=255)
                          gCCCTCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTgg                                                                                                                                               >  5:113322/1‑127 (MQ=255)
                          gCCCTCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTgg                                                                                                                                               <  6:113322/127‑1 (MQ=255)
                          gCCCTCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAg                                                                                                                                            <  2:14181/130‑1 (MQ=255)
                              tCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGc                                                                                                                     >  5:153390/1‑149 (MQ=255)
                                gACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCt                                                                                                                   <  7:221499/149‑1 (MQ=255)
                                     acacCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCCCTGGCAGTGAAAGCGGtt                                                                                                                                 >  8:131016/1‑130 (MQ=255)
                                       acCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGc                                                                                                            >  3:141269/1‑149 (MQ=255)
                                          gggATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCTAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGGTGCGCCCGGTGGCCTGCAGAGCGaa                                                                                                         <  3:259993/149‑1 (MQ=255)
                                          gggATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGaa                                                                                                         <  6:153390/149‑1 (MQ=255)
                                             aTGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAggg                                                                                                      >  8:237457/1‑149 (MQ=255)
                                                tCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCtg                                                                                                   >  7:37841/1‑149 (MQ=255)
                                                   ttttCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCagag                                                                                                             <  2:307287/136‑1 (MQ=255)
                                                   ttttCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCagag                                                                                                             >  1:307287/1‑136 (MQ=255)
                                                   ttttCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCtgt                                                                                                  >  2:191894/1‑147 (MQ=255)
                                                   ttttCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCtgt                                                                                                  <  1:191894/147‑1 (MQ=255)
                                                           tAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGAt                                                                                        <  4:141269/149‑1 (MQ=255)
                                                                ggCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCt                                                                                    >  5:248447/1‑148 (MQ=255)
                                                                       ttGTATCAAGGCTTTTTCCAGGCGCGGATCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCt                                                                            >  2:81479/1‑149 (MQ=255)
                                                                            tCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCgg                                                                                            >  8:98683/1‑128 (MQ=255)
                                                                            tCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCgg                                                                                            <  7:98683/128‑1 (MQ=255)
                                                                             cAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTg                                                                        >  8:217721/1‑147 (MQ=255)
                                                                              aaGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAg                                                                     >  5:3445/1‑149 (MQ=255)
                                                                                 gCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAgggg                                                                  >  7:156241/1‑149 (MQ=255)
                                                                                  cTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGt                                                                 <  8:156241/149‑1 (MQ=255)
                                                                                   tttttCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCa                                                                      >  7:251294/1‑143 (MQ=255)
                                                                                   tttttCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCa                                                                      <  8:251294/143‑1 (MQ=255)
                                                                                         cAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTACCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAggg                                                                   >  7:258833/1‑140 (MQ=255)
                                                                                         cAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTACCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAggg                                                                   >  7:127849/1‑140 (MQ=255)
                                                                                         cAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTACCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAggg                                                                   <  8:127849/140‑1 (MQ=255)
                                                                                         cAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTACCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAggg                                                                   <  8:258833/140‑1 (MQ=255)
                                                                                          aGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCCCTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAAGTGGaa                                                         >  7:74673/1‑149 (MQ=255)
                                                                                          aGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCgg                                                                                            <  4:123548/114‑1 (MQ=255)
                                                                                          aGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCgg                                                                                            >  3:123548/1‑114 (MQ=255)
                                                                                           ggCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTc                                                                                              >  1:347680/1‑111 (MQ=255)
                                                                                           ggCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTc                                                                                              <  2:347680/111‑1 (MQ=255)
                                                                                                      gCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAgg                                                                    <  2:338657/126‑1 (MQ=255)
                                                                                                      gCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGAt                                             <  7:237457/149‑1 (MQ=255)
                                                                                                      gCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGAt                                             <  8:37841/149‑1 (MQ=255)
                                                                                                            ccGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGc                                                                                                                                     >  2:56142/1‑55 (MQ=255)
                                                                                                            ccGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGc                                                                                                                                     <  1:56142/55‑1 (MQ=255)
                                                                                                            ccGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTg                                                                        >  6:156563/1‑116 (MQ=255)
                                                                                                               gcgtCGGGGCGGCGAGTGGTTTCGCGTGGGGGGGGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTtgctg                                    <  8:74673/146‑1 (MQ=255)
                                                                                                                   cGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTtg                                       <  4:29342/142‑1 (MQ=255)
                                                                                                                   cGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTtg                                       >  3:29342/1‑142 (MQ=255)
                                                                                                                                 gTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCg                                               >  4:116911/1‑120 (MQ=255)
                                                                                                                                 gTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCg                                               <  3:116911/120‑1 (MQ=255)
                                                                                                                                 gTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAg                  <  1:81479/149‑1 (MQ=255)
                                                                                                                                  tttCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCgg                              >  2:59856/1‑136 (MQ=255)
                                                                                                                                  tttCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCgg                              <  1:59856/136‑1 (MQ=255)
                                                                                                                                  tttCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAg                  >  1:222226/1‑148 (MQ=255)
                                                                                                                                       cgTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGc             >  4:251989/1‑148 (MQ=255)
                                                                                                                                        gTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCgg                              <  2:186611/130‑1 (MQ=255)
                                                                                                                                        gTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCgg                              >  1:186611/1‑130 (MQ=255)
                                                                                                                                                 gCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCgg  >  2:6688/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AACACTTCTTTGCCTACGCCTGTTTCGCCCTCGACCAACACCGGGATGTCCTTTTCCAATAGCAGGCCGGCTTGTATCAAGGCTTTTTCCAGGCGCGGCTCGGCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGG  >  NC_002947/2577388‑2577681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑