| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,595,170 | G→T | 11.1% | intergenic (+29/‑112) | PP_5511 → / → PP_2273 | hypothetical protein/DNA polymerase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,595,170 | 0 | G | T | 11.1% | 178.1 / 12.8 | 63 | intergenic (+29/‑112) | PP_5511/PP_2273 | hypothetical protein/DNA polymerase I |
| Reads supporting (aligned to +/- strand): ref base G (26/30); new base T (5/2); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01 | |||||||||||
GATCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAG > NC_002947/2595030‑2595305 | gATCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCTGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCt > 2:110108/1‑149 (MQ=255)gATCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGAcc < 5:86256/148‑1 (MQ=255)gATCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCATTCGTTTCGaggt > 8:192059/1‑146 (MQ=255) aTCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGt > 4:108848/1‑141 (MQ=255) aTCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGt < 3:108848/141‑1 (MQ=255) aTGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTc < 2:299300/146‑1 (MQ=255) aTGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTc > 1:299300/1‑146 (MQ=255) aTGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGaa < 1:309402/149‑1 (MQ=255) cTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAAt < 8:182485/149‑1 (MQ=255) ggCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATgg < 7:65770/148‑1 (MQ=255) gAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTc > 1:308702/1‑149 (MQ=255) aaGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTcc < 6:37717/149‑1 (MQ=255) aGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTctt < 7:192059/149‑3 (MQ=38) ccTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTCCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGaaa < 2:177999/133‑1 (MQ=255) ccTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTACCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGaaa > 1:177999/1‑133 (MQ=255) ggCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCGCGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTg > 1:242616/1‑140 (MQ=255) ggCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTg < 2:242616/140‑1 (MQ=255) cTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCg > 8:126593/1‑114 (MQ=255) cTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCg < 7:126593/114‑1 (MQ=255) tACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCg < 6:207724/147‑1 (MQ=255) cACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATc > 4:344525/1‑149 (MQ=255) aCGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATc > 7:209039/1‑148 (MQ=255) gCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGAGTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCAtt < 1:110108/149‑1 (MQ=255) gCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCAtt < 8:209039/149‑1 (MQ=255) gtgATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCtt < 3:344525/148‑1 (MQ=255) cccTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGAccgc > 2:27898/1‑95 (MQ=255) ccTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCg > 8:113038/1‑148 (MQ=255) aCCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCa > 3:184150/1‑120 (MQ=255) aCCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCa < 4:184150/120‑1 (MQ=255) cGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAAc > 4:37976/1‑149 (MQ=255) aCGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCGTTGTCATCGAAGACACGGCAACTggg < 2:308702/149‑1 (MQ=255) gagctTCCGAGAGCTGTGGCATACGCCTACTCCCTGCTTACTGGGGTACCAATGCATGAAGGCTTTGTGTTGGACCTCGCATCGAATGGGTTACTGGAGGAAGTCTCAGTGATGCATTGCCTTGTCATCGAAGACACGGCAACTGGGGa < 8:236127/146‑1 (MQ=255) gCTCTACCGAGAGCTGCGGCATACGCCGACCCCCTGCTGACTTGGTGACCAATGCAAGAAACACTTCTGTTCGACCTAGAAACGAATGGGTTCCTGGAGTGTCTCTCCGTCATCAATTGCCTTGTCATCGATGACACGGCAACTGGGGa < 8:271076/149‑1 (MQ=255) ttCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGAc > 4:168033/1‑71 (MQ=255) ttCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGAc < 3:168033/71‑1 (MQ=255) gagaGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTgggg < 1:82782/140‑1 (MQ=255) gagaGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTgggg > 2:82782/1‑140 (MQ=255) agagCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGa > 5:120300/1‑148 (MQ=255) cTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTc < 3:37976/149‑1 (MQ=255) tgtgGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCATGGAGGCAGTCTCCGTAAACCATTGCCTTGTCAACGAAGCAACGCCAACTGGGGACGTTAAGAAATTcc > 6:265885/1‑149 (MQ=255) tACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGa > 5:218889/1‑113 (MQ=255) tACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGa < 6:218889/113‑1 (MQ=255) tACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTgg > 3:64925/1‑149 (MQ=255) gCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCt > 2:302146/1‑149 (MQ=255) ccGACCCCCTGCTTACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTc < 5:265885/149‑1 (MQ=255) ccccTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGc > 6:95003/1‑149 (MQ=255) ctgACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATgg < 5:95003/148‑1 (MQ=255) tgACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa < 7:113038/149‑1 (MQ=255) gACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAg > 6:153401/1‑149 (MQ=255) aCTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa < 6:120300/147‑1 (MQ=255) aCTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa < 1:302146/147‑1 (MQ=255) gggACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATc < 5:322785/85‑1 (MQ=255) gggACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTACTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATc > 6:322785/1‑85 (MQ=255) aaTGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATgg > 2:188718/1‑134 (MQ=255) aaTGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATgg < 1:188718/134‑1 (MQ=255) ctccaggAACCCATTCGTTTCGAGGTGGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTc > 1:186539/7‑147 (MQ=37) ccaggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCTTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAg > 1:12185/5‑148 (MQ=37) ccaggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCa > 1:110885/5‑128 (MQ=37) ccaggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCa < 2:110885/124‑1 (MQ=37) aaGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAg > 1:295610/1‑149 (MQ=255) ggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGATCCAGGAg > 7:159880/2‑148 (MQ=38) cTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa < 1:39291/123‑1 (MQ=255) cTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa > 2:39291/1‑123 (MQ=255) | GATCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAG > NC_002947/2595030‑2595305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |