Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,595,177 C→G 11.1% intergenic (+36/‑105) PP_5511 → / → PP_2273 hypothetical protein/DNA polymerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,595,1770CG11.1% 142.8 / 8.7 54intergenic (+36/‑105)PP_5511/PP_2273hypothetical protein/DNA polymerase I
Reads supporting (aligned to +/- strand):  ref base C (22/26);  new base G (4/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

GATCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGCT  >  NC_002947/2595030‑2595324
                                                                                                                                                   |                                                                                                                                                   
gATCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCTGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCt                                                                                                                                                    >  2:110108/1‑149 (MQ=255)
gATCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGAcc                                                                                                                                                     <  5:86256/148‑1 (MQ=255)
    aTGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTc                                                                                                                                                   <  2:299300/146‑1 (MQ=255)
    aTGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTc                                                                                                                                                   >  1:299300/1‑146 (MQ=255)
    aTGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGaa                                                                                                                                                <  1:309402/149‑1 (MQ=255)
          cTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAAt                                                                                                                                          <  8:182485/149‑1 (MQ=255)
             ggCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATgg                                                                                                                                        <  7:65770/148‑1 (MQ=255)
                gAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTc                                                                                                                                    >  1:308702/1‑149 (MQ=255)
                 aaGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTcc                                                                                                                                   <  6:37717/149‑1 (MQ=255)
                  aGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTctt                                                                                                                                  <  7:192059/149‑3 (MQ=38)
                     ccTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTCCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGaaa                                                                                                                                               <  2:177999/133‑1 (MQ=255)
                     ccTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTACCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGaaa                                                                                                                                               >  1:177999/1‑133 (MQ=255)
                            ggCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCGCGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTg                                                                                                                                 >  1:242616/1‑140 (MQ=255)
                            ggCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTg                                                                                                                                 <  2:242616/140‑1 (MQ=255)
                                  tACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCg                                                                                                                    <  6:207724/147‑1 (MQ=255)
                                     cACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATc                                                                                                               >  4:344525/1‑149 (MQ=255)
                                      aCGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATc                                                                                                               >  7:209039/1‑148 (MQ=255)
                                         gCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGAGTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCAtt                                                                                                           <  1:110108/149‑1 (MQ=255)
                                         gCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCAtt                                                                                                           <  8:209039/149‑1 (MQ=255)
                                               gtgATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCtt                                                                                                      <  3:344525/148‑1 (MQ=255)
                                                     cccTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGAccgc                                                                                                                                                   >  2:27898/1‑95 (MQ=255)
                                                      ccTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCg                                                                                               >  8:113038/1‑148 (MQ=255)
                                                                aCCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCa                                                                                                                 <  4:184150/120‑1 (MQ=255)
                                                                aCCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCa                                                                                                                 >  3:184150/1‑120 (MQ=255)
                                                                  cGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAAc                                                                                  >  4:37976/1‑149 (MQ=255)
                                                                      aCGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCGTTGTCATCGAAGACACGGCAACTggg                                                                              <  2:308702/149‑1 (MQ=255)
                                                                        gagctTCCGAGAGCTGTGGCATACGCCTACTCCCTGCTTACTGGGGTACCAATGCATGAAGGCTTTGTGTTGGACCTCGCATCGAATGGGTTACTGGAGGAAGTCTCAGTGATGCATTGCCTTGTCATCGAAGACACGGCAACTGGGGa                                                                            <  8:236127/146‑1 (MQ=255)
                                                                        gCTCTACCGAGAGCTGCGGCATACGCCGACCCCCTGCTGACTTGGTGACCAATGCAAGAAACACTTCTGTTCGACCTAGAAACGAATGGGTTCCTGGAGTGTCTCTCCGTCATCAATTGCCTTGTCATCGATGACACGGCAACTGGGGa                                                                            <  8:271076/149‑1 (MQ=255)
                                                                                gagaGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTgggg                                                                             >  2:82782/1‑140 (MQ=255)
                                                                                gagaGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTgggg                                                                             <  1:82782/140‑1 (MQ=255)
                                                                                 agagCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGa                                                                    >  5:120300/1‑148 (MQ=255)
                                                                                     cTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTc                                                               <  3:37976/149‑1 (MQ=255)
                                                                                      tgtgGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCATGGAGGCAGTCTCCGTAAACCATTGCCTTGTCAACGAAGCAACGCCAACTGGGGACGTTAAGAAATTcc                                                              >  6:265885/1‑149 (MQ=255)
                                                                                             tACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGa                                                                                           <  6:218889/113‑1 (MQ=255)
                                                                                             tACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGa                                                                                           >  5:218889/1‑113 (MQ=255)
                                                                                             tACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTgg                                                       >  3:64925/1‑149 (MQ=255)
                                                                                                gCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCt                                                    >  2:302146/1‑149 (MQ=255)
                                                                                                 ccGACCCCCTGCTTACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTc                                                   <  5:265885/149‑1 (MQ=255)
                                                                                                      ccccTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGc                                              >  6:95003/1‑149 (MQ=255)
                                                                                                            ctgACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATgg                                         <  5:95003/148‑1 (MQ=255)
                                                                                                             tgACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa                                       <  7:113038/149‑1 (MQ=255)
                                                                                                              gACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAg                                      >  6:153401/1‑149 (MQ=255)
                                                                                                               aCTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa                                       <  1:302146/147‑1 (MQ=255)
                                                                                                               aCTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa                                       <  6:120300/147‑1 (MQ=255)
                                                                                                                    gggACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATc                                                                                                <  5:322785/85‑1 (MQ=255)
                                                                                                                    gggACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTACTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATc                                                                                                >  6:322785/1‑85 (MQ=255)
                                                                                                                          aaTGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATgg                                         >  2:188718/1‑134 (MQ=255)
                                                                                                                          aaTGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATgg                                         <  1:188718/134‑1 (MQ=255)
                                                                                                                           ctccaggAACCCATTCGTTTCGAGGTGGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTc                           >  1:186539/7‑147 (MQ=37)
                                                                                                                             ccaggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCTTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAg                        >  1:12185/5‑148 (MQ=37)
                                                                                                                             ccaggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCa                                            <  2:110885/124‑1 (MQ=37)
                                                                                                                             ccaggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCa                                            >  1:110885/5‑128 (MQ=37)
                                                                                                                               aaGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAg                     >  1:295610/1‑149 (MQ=255)
                                                                                                                                ggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGATCCAGGAg                     >  7:159880/2‑148 (MQ=38)
                                                                                                                                       cTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa                                       >  2:39291/1‑123 (MQ=255)
                                                                                                                                       cTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa                                       <  1:39291/123‑1 (MQ=255)
                                                                                                                                                   cTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGCt  <  3:333351/148‑1 (MQ=255)
                                                                                                                                                   cTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGCt  >  4:333351/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATCATGATCCTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGCT  >  NC_002947/2595030‑2595324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑