Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,595,184 C→A 11.1% intergenic (+43/‑98) PP_5511 → / → PP_2273 hypothetical protein/DNA polymerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,595,1840CA11.1% 154.0 / 9.8 54intergenic (+43/‑98)PP_5511/PP_2273hypothetical protein/DNA polymerase I
Reads supporting (aligned to +/- strand):  ref base C (22/26);  new base A (4/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-01

CTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGCTTCATC  >  NC_002947/2595040‑2595329
                                                                                                                                                |                                                                                                                                                 
cTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAAt                                                                                                                                               <  8:182485/149‑1 (MQ=255)
   ggCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATgg                                                                                                                                             <  7:65770/148‑1 (MQ=255)
      gAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTc                                                                                                                                         >  1:308702/1‑149 (MQ=255)
       aaGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTcc                                                                                                                                        <  6:37717/149‑1 (MQ=255)
        aGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTctt                                                                                                                                       <  7:192059/149‑3 (MQ=38)
                  ggCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCGCGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTg                                                                                                                                      >  1:242616/1‑140 (MQ=255)
                  ggCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTg                                                                                                                                      <  2:242616/140‑1 (MQ=255)
                        tACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCg                                                                                                                         <  6:207724/147‑1 (MQ=255)
                           cACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATc                                                                                                                    >  4:344525/1‑149 (MQ=255)
                            aCGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATc                                                                                                                    >  7:209039/1‑148 (MQ=255)
                               gCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGAGTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCAtt                                                                                                                <  1:110108/149‑1 (MQ=255)
                               gCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCAtt                                                                                                                <  8:209039/149‑1 (MQ=255)
                                     gtgATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCtt                                                                                                           <  3:344525/148‑1 (MQ=255)
                                            ccTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCg                                                                                                    >  8:113038/1‑148 (MQ=255)
                                                      aCCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCa                                                                                                                      >  3:184150/1‑120 (MQ=255)
                                                      aCCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCa                                                                                                                      <  4:184150/120‑1 (MQ=255)
                                                        cGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAAc                                                                                       >  4:37976/1‑149 (MQ=255)
                                                            aCGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCGTTGTCATCGAAGACACGGCAACTggg                                                                                   <  2:308702/149‑1 (MQ=255)
                                                              gagctTCCGAGAGCTGTGGCATACGCCTACTCCCTGCTTACTGGGGTACCAATGCATGAAGGCTTTGTGTTGGACCTCGCATCGAATGGGTTACTGGAGGAAGTCTCAGTGATGCATTGCCTTGTCATCGAAGACACGGCAACTGGGGa                                                                                 <  8:236127/146‑1 (MQ=255)
                                                              gCTCTACCGAGAGCTGCGGCATACGCCGACCCCCTGCTGACTTGGTGACCAATGCAAGAAACACTTCTGTTCGACCTAGAAACGAATGGGTTCCTGGAGTGTCTCTCCGTCATCAATTGCCTTGTCATCGATGACACGGCAACTGGGGa                                                                                 <  8:271076/149‑1 (MQ=255)
                                                                      gagaGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTgggg                                                                                  <  1:82782/140‑1 (MQ=255)
                                                                      gagaGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTgggg                                                                                  >  2:82782/1‑140 (MQ=255)
                                                                       agagCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGa                                                                         >  5:120300/1‑148 (MQ=255)
                                                                           cTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTc                                                                    <  3:37976/149‑1 (MQ=255)
                                                                            tgtgGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCATGGAGGCAGTCTCCGTAAACCATTGCCTTGTCAACGAAGCAACGCCAACTGGGGACGTTAAGAAATTcc                                                                   >  6:265885/1‑149 (MQ=255)
                                                                                   tACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGa                                                                                                >  5:218889/1‑113 (MQ=255)
                                                                                   tACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGa                                                                                                <  6:218889/113‑1 (MQ=255)
                                                                                   tACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTgg                                                            >  3:64925/1‑149 (MQ=255)
                                                                                      gCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCt                                                         >  2:302146/1‑149 (MQ=255)
                                                                                       ccGACCCCCTGCTTACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTc                                                        <  5:265885/149‑1 (MQ=255)
                                                                                            ccccTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGc                                                   >  6:95003/1‑149 (MQ=255)
                                                                                                  ctgACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATgg                                              <  5:95003/148‑1 (MQ=255)
                                                                                                   tgACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa                                            <  7:113038/149‑1 (MQ=255)
                                                                                                    gACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAg                                           >  6:153401/1‑149 (MQ=255)
                                                                                                     aCTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa                                            <  6:120300/147‑1 (MQ=255)
                                                                                                     aCTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa                                            <  1:302146/147‑1 (MQ=255)
                                                                                                          gggACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATc                                                                                                     <  5:322785/85‑1 (MQ=255)
                                                                                                          gggACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTACTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATc                                                                                                     >  6:322785/1‑85 (MQ=255)
                                                                                                                aaTGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATgg                                              <  1:188718/134‑1 (MQ=255)
                                                                                                                aaTGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATgg                                              >  2:188718/1‑134 (MQ=255)
                                                                                                                 ctccaggAACCCATTCGTTTCGAGGTGGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTc                                >  1:186539/7‑147 (MQ=37)
                                                                                                                   ccaggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCTTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAg                             >  1:12185/5‑148 (MQ=37)
                                                                                                                   ccaggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCa                                                 >  1:110885/5‑128 (MQ=37)
                                                                                                                   ccaggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCa                                                 <  2:110885/124‑1 (MQ=37)
                                                                                                                     aaGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAg                          >  1:295610/1‑149 (MQ=255)
                                                                                                                      ggAACCCATTCGTTTCGAGGTCGAACAGAAGGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGATCCAGGAg                          >  7:159880/2‑148 (MQ=38)
                                                                                                                             cTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa                                            >  2:39291/1‑123 (MQ=255)
                                                                                                                             cTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGa                                            <  1:39291/123‑1 (MQ=255)
                                                                                                                                         cTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGCt       >  4:333351/1‑148 (MQ=255)
                                                                                                                                         cTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGCt       <  3:333351/148‑1 (MQ=255)
                                                                                                                                          tCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGc        >  2:80300/1‑146 (MQ=255)
                                                                                                                                          tCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGc        <  1:80300/146‑1 (MQ=255)
                                                                                                                                           cGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGCTTCa    >  1:306653/1‑149 (MQ=255)
                                                                                                                                             aaaCGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGCTTCATc  <  4:64925/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CTGGGCGAAGCCCTCAAGGGCCTGTACCACGGCTTTTGTGATGCCCTGGCCGATACCGGCACGCTCTTCCGAGAGCTGTGGCATACGCCGACCCCCTGCTGACTGGGGGACCAATGCAAGAACCCCTTCTGTTCGACCTCGAAACGAATGGGTTCCTGGAGGCAGTCTCCGTCATCCATTGCCTTGTCATCGAAGACACGGCAACTGGGGACGTTAAGAAATTCCCGCCTGGCCTCATCGCCATGGGAGTCAAATGGCTCCAGGAGCAACATTCCCAAGGCCGCTTCATC  >  NC_002947/2595040‑2595329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑