Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,630,704 G→A 8.0% D57N (GAC→AAC)  lon‑II → DNA‑binding ATP‑dependent protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,630,7040GA 8.0% 141.7 / 2.1 50D57N (GAC→AAC) lon‑IIDNA‑binding ATP‑dependent protease
Reads supporting (aligned to +/- strand):  ref base G (24/22);  new base A (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01

GTCGTTGTTTACCCGCACATGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAGCGCTTCTCCGAAGTTGAAG  >  NC_002947/2630578‑2630845
                                                                                                                              |                                                                                                                                             
gTCGTTGTTTACCCGCACATGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGccc                                                                                                                          <  8:196575/148‑1 (MQ=255)
gTCGTTGTTTACCCGCACATGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCt                                                                                                                         >  1:185908/1‑149 (MQ=255)
    ttgttTACCCGCACATGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTAc                                                                                                                     >  3:309600/1‑149 (MQ=255)
       tttACCCGCACATGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACcgc                                                                                                                  >  6:293155/1‑149 (MQ=255)
           cccGCACATGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTc                                                                                                               <  6:243836/148‑1 (MQ=255)
           cccGCACATGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCg                                                                                                              >  5:88132/1‑149 (MQ=255)
            ccGCACATGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCgg                                                                                                             >  7:167674/1‑149 (MQ=255)
               cacaTGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTAc                                                                                                          >  3:128490/1‑149 (MQ=255)
                acaTGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTAcc                                                                                                         >  6:301266/1‑149 (MQ=255)
                   tGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGtt                                                                                                      <  2:32380/149‑1 (MQ=255)
                    ggTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACTcc                                                                                                                           <  4:130886/127‑1 (MQ=255)
                    ggTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGcc                                                                                                                           >  3:130886/1‑127 (MQ=255)
                     gTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCcgacga                                                                                                                                              <  2:67307/107‑1 (MQ=255)
                     gTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCcgacga                                                                                                                                              >  1:67307/1‑107 (MQ=255)
                      ttATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGcc                                                                                                   >  3:261907/1‑149 (MQ=255)
                             gCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGtgc                                                                                            >  5:172477/1‑149 (MQ=255)
                                    gTGGGGCGCGAAAAGTCCATCGTAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTAGCGTTGCCACCGTGCTGCAAc                                                                                      <  5:85859/148‑1 (MQ=255)
                                       gggCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAActgct                                                                                  <  4:128490/149‑1 (MQ=255)
                                       gggCGCGAAAAGTCCAACGAACCCCTCGAGGCGGCGATGACGGGCGAAAAGAAGAACCAACTCCTGGCCAAGAAGAATCCAACCAACGAAGAACAGGGCGAAGACCCCATGTACCGCGACGGAACCAATGCCACCGAGCTGCaaaagc                                                                                   >  8:218116/1‑144 (MQ=255)
                                            cgAAAAGTCCATCGTAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGc                                                                             <  5:293155/149‑1 (MQ=255)
                                                aaGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGcc                                                                         <  4:261907/149‑1 (MQ=255)
                                                   tCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGt                                                                                                                       <  1:301958/100‑1 (MQ=255)
                                                   tCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGt                                                                                                                       >  2:301958/1‑100 (MQ=255)
                                                   tCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGa                                                                      <  8:167674/149‑1 (MQ=255)
                                                      aTCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATgg                                                                   >  3:281192/1‑149 (MQ=255)
                                                         gAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGATGCTGCCTGATGGCAc                                                                <  2:185908/149‑1 (MQ=255)
                                                              ccTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACAACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAg                                                                              <  8:44995/130‑1 (MQ=255)
                                                              ccTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACAACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAg                                                                              >  7:44995/1‑130 (MQ=255)
                                                              ccTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACAACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAg                                                                              >  7:50031/1‑130 (MQ=255)
                                                              ccTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACAACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAg                                                                              <  8:50031/130‑1 (MQ=255)
                                                                  gtggcggtGTTGACGGGCTAAAAGCATATCCTACTGGTGGCCCATAAGTATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAgg                                                        <  7:218116/146‑1 (MQ=255)
                                                                  gAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAg                                                                                                                                 <  8:175792/75‑1 (MQ=255)
                                                                  gAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAg                                                                                                                                 >  7:175792/1‑75 (MQ=255)
                                                                  gAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGt                                                       >  2:339007/1‑149 (MQ=255)
                                                                             gACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAgg                                            >  4:58314/1‑149 (MQ=255)
                                                                                     aaaaGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGagcag                                    <  5:301266/149‑1 (MQ=255)
                                                                                         gCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCgtg                                <  6:172477/149‑3 (MQ=255)
                                                                                            gATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTg                             >  2:103899/1‑149 (MQ=255)
                                                                                               ccTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCgg                          <  4:309600/149‑1 (MQ=255)
                                                                                                   ctgctgGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAgg                                            >  4:284906/1‑127 (MQ=255)
                                                                                                         gCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAgg                                            >  2:150915/1‑121 (MQ=255)
                                                                                                           ccAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCt                                                                        <  7:67334/91‑1 (MQ=255)
                                                                                                           ccAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCt                                                                        >  8:67334/1‑91 (MQ=255)
                                                                                                           ccAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAGCGCTtct              >  4:255091/1‑149 (MQ=255)
                                                                                                            cAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAGCGCTtctc             <  6:88132/149‑1 (MQ=255)
                                                                                                              gaagaaTCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAGCGCTTCTcc            >  8:19647/1‑148 (MQ=255)
                                                                                                                agaaTCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAGCGCTTCTCCGaa         <  1:339007/149‑1 (MQ=255)
                                                                                                                 gaaTCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAgc                    <  2:226919/137‑1 (MQ=255)
                                                                                                                 gaaTCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAgc                    >  1:226919/1‑137 (MQ=255)
                                                                                                                       aGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGGAGCGGGGTGCGGTCGAGCGCTTCTCCGAAGTTg     >  5:336625/1‑146 (MQ=255)
                                                                                                                       aGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGGAGCGGGGTGCGGTCGAGCGCTTCTCCGAAGTTGaa   <  6:336625/148‑1 (MQ=255)
                                                                                                                       aGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAGCGCTTCTCCGAAGTTGAAg  <  3:255091/149‑1 (MQ=255)
                                                                                                                              gacgaTCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAGCGCt                 <  4:13821/127‑1 (MQ=255)
                                                                                                                              gacgaTCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAGCGCt                 >  3:13821/1‑127 (MQ=255)
                                                                                                                              |                                                                                                                                             
GTCGTTGTTTACCCGCACATGGTTATCCCGCTGTTCGTGGGGCGCGAAAAGTCCATCGAAGCCCTCGAGGCGGCGATGACGGGCGAAAAGCAGATCCTACTGCTGGCCCAGAAGAATCCAGCCGACGACGATCCGGGCGAGGACGCCCTGTACCGCGTCGGTACCGTTGCCACCGTGCTGCAACTGCTGAAGCTGCCTGATGGCACCGTCAAGGTGCTGGTCGAGGGCGAGCAGCGGGGTGCGGTCGAGCGCTTCTCCGAAGTTGAAG  >  NC_002947/2630578‑2630845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑