Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 74,159 A→T 7.3% K212M (AAG→ATG)  PP_0063 → lipid A biosynthesis lauroyl acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294774,1590AT 7.3% 202.5 / 5.5 68K212M (AAG→ATG) PP_0063lipid A biosynthesis lauroyl acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (31/32);  new base T (3/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

GCGTGGCACCGTCGACCAAGGAAGGCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGATGTGAATGTGTCGGCGGCGG  >  NC_002947/74012‑74305
                                                                                                                                                   |                                                                                                                                                  
gcgTGGCACCGTCGACCAAGGAAGGCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAg                                                                                                                                                   <  5:160231/149‑1 (MQ=255)
           tCGACCAAGGAAGGCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGaa                                                                                                                                        <  5:103961/149‑1 (MQ=255)
              aCCAAGGAAGGCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACAt                                                                                                                                     >  8:330706/1‑149 (MQ=255)
               ccAAGGAAGGCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGt                                                                                                                                                  >  1:322674/1‑135 (MQ=255)
               ccAAGGAAGGCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGt                                                                                                                                                  <  2:322674/135‑1 (MQ=255)
                cAAGGAAGGCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGc                                                                                                                                   <  8:165642/149‑1 (MQ=255)
                   ggaaggCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTgg                                                                                                                                <  6:14897/149‑1 (MQ=255)
                   ggaaggCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTgg                                                                                                                                >  6:119102/1‑149 (MQ=255)
                   ggaaggCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGCGGCCAGGTGGGTATTCATGCCAACCCGGAGCCGGCGGATTCGGCGGGCGTGTTCTTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAATTTCGTGCGGAAAATGATgg                                                                                                                                >  7:51673/1‑149 (MQ=255)
                     aaggCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGcc                                                                                                                              <  5:316306/149‑1 (MQ=255)
                           tCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTgg                                                                                                                         >  6:25352/1‑148 (MQ=255)
                                aGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAgg                                                                                                                    <  3:194746/148‑1 (MQ=255)
                                      aTCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCaa                                                                                                                                                    >  3:325226/1‑110 (MQ=255)
                                      aTCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCaa                                                                                                                                                    <  4:325226/110‑1 (MQ=255)
                                      aTCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAg                                                                                                              >  8:157321/1‑148 (MQ=255)
                                      aTCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAg                                                                                                              <  7:157321/148‑1 (MQ=255)
                                      aTCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAg                                                                                                              <  7:330706/148‑1 (MQ=255)
                                       tCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAgg                                                                                                                    <  3:46902/141‑1 (MQ=255)
                                       tCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAgg                                                                                                                    >  4:46902/1‑141 (MQ=255)
                                       tCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGgt                                                                                                            <  5:25352/149‑1 (MQ=255)
                                           ggAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGgtgtgt                                                                                                        >  8:263330/1‑149 (MQ=255)
                                               gTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCt                                                                                                    <  3:172336/149‑1 (MQ=255)
                                               gTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCt                                                                                                    >  4:172336/1‑149 (MQ=255)
                                                          tggCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGgt                                                                                                            <  5:42605/130‑1 (MQ=255)
                                                          tggCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGgt                                                                                                            >  6:42605/1‑130 (MQ=255)
                                                             ccAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCATGTTCGGCACGAACTTGCTg                                                                                                                                 <  4:263052/106‑1 (MQ=255)
                                                             ccAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCATGTTCGGCACGAACTTGCTg                                                                                                                                 >  3:263052/1‑106 (MQ=255)
                                                               aGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTg                                                                                    >  6:139438/1‑149 (MQ=255)
                                                                 gTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGcc                                                                                  <  6:137114/149‑1 (MQ=255)
                                                                        ttCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATgg                                                                            <  5:119102/148‑1 (MQ=255)
                                                                           cTGCCGACCCGGATCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGAt                                                                              <  7:12233/143‑1 (MQ=255)
                                                                           cTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCg                                                                        <  2:47769/149‑1 (MQ=255)
                                                                           cTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCCTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGAt                                                                              >  8:12233/1‑143 (MQ=255)
                                                                            tGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGc                                                                                                                               >  7:95085/1‑93 (MQ=255)
                                                                            tGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGc                                                                                                                               <  8:95085/93‑1 (MQ=255)
                                                                              ccGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGc                                                                     <  5:139438/149‑1 (MQ=255)
                                                                                 aCCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGgt                                                                                                            >  7:12560/1‑107 (MQ=255)
                                                                                 aCCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGgt                                                                                                            <  8:12560/107‑1 (MQ=255)
                                                                                   ccGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTc                                                                  >  8:186417/1‑147 (MQ=255)
                                                                                   ccGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACAGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTc                                                                  >  8:182904/1‑147 (MQ=255)
                                                                                    cGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGCCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAg                                                               <  7:186417/149‑1 (MQ=255)
                                                                                                aaTCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCaa                                                                >  1:250567/1‑136 (MQ=255)
                                                                                                aaTCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCaa                                                                <  2:250567/136‑1 (MQ=255)
                                                                                                    ggcgggcgTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCTGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTg                                               >  8:171967/1‑149 (MQ=255)
                                                                                                              ttCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGgt                                                                                                            >  5:270819/1‑78 (MQ=255)
                                                                                                              ttCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGgt                                                                                                            <  6:270819/78‑1 (MQ=255)
                                                                                                                cGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAagag                                    <  7:182904/148‑1 (MQ=255)
                                                                                                                 gTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGAt                                  >  7:245968/1‑149 (MQ=255)
                                                                                                                  tACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATg                                 >  1:67564/1‑149 (MQ=255)
                                                                                                                      gTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTgc                                              >  7:98620/1‑132 (MQ=255)
                                                                                                                      gTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTgc                                              <  8:98620/132‑1 (MQ=255)
                                                                                                                        tCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGGATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTc                                                         >  1:111683/1‑119 (MQ=255)
                                                                                                                        tCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTc                                                         <  2:111683/119‑1 (MQ=255)
                                                                                                                         ccTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCAACCGCAGCGCATGCAGGaa                                                                              <  6:342331/97‑2 (MQ=255)
                                                                                                                         ccTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCAACCGCAGCGCATGCAGGaa                                                                              >  5:342331/1‑96 (MQ=255)
                                                                                                                          cTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCAc                         >  1:63881/1‑149 (MQ=255)
                                                                                                                           tCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCAcc                        >  2:77505/1‑149 (MQ=255)
                                                                                                                           tCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGACCGTGGCAAGCCGGTAGGTGAGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTAAAGCAcc                        >  2:250719/1‑149 (MQ=255)
                                                                                                                              gCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGAt                     <  8:245968/149‑1 (MQ=255)
                                                                                                                              gCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGAt                     <  2:63881/149‑1 (MQ=255)
                                                                                                                                   cAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGATGTGaa                <  1:77505/149‑1 (MQ=255)
                                                                                                                                    aGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGATGTGAAt               <  7:263330/149‑1 (MQ=255)
                                                                                                                                       caccggccAGCATGTTCGGCACGAACTTGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGATGTGAAtgtg            >  2:74918/7‑149 (MQ=255)
                                                                                                                                            aCCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGGGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGAt                     >  4:216856/1‑135 (MQ=255)
                                                                                                                                              cAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGATGTGAAt               >  7:240026/1‑139 (MQ=255)
                                                                                                                                              cAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGATGTGAATGTGTcggcgg     >  5:126357/1‑149 (MQ=255)
                                                                                                                                              cAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGGGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGATGTGAAt               <  8:240026/139‑1 (MQ=255)
                                                                                                                                               aGCATGTTCGGCACGAACTTGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGAt                     <  5:19782/132‑1 (MQ=255)
                                                                                                                                               aGCATGTTCGGCACGAACTTGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGAt                     >  6:19782/1‑132 (MQ=255)
                                                                                                                                                 cAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGATGTGAATGTGTcggcggcgg  >  4:286956/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGTGGCACCGTCGACCAAGGAAGGCATCCTCAGCGTGATCAAGGAAGTGCGCCGTGGTGGCCAGGTGGGTATTCCTGCCGACCCGGAGCCGGCGGAATCGGCGGGCGTGTTCGTACCGTTCCTCGGCACCCAGGCGCTGACCAGCAAGTTCGTGCCGAACATGCTGGCCGGTGGCAAGGCGGTAGGTGTGTTCCTGCATGCGCTGCGGTTGCCGGATGGCTCGGGCTTCAAGGTGTTCCTCGAAGCTGCGCCGGAAGAGATGTACAGCACCGATGTGAATGTGTCGGCGGCGG  >  NC_002947/74012‑74305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑