Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,658,381 C→G 6.9% A140A (GCG→GCC)  PP_2330 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,658,3810CG 6.9% 184.3 / 2.9 58A140A (GCG→GCC) PP_2330hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/24);  new base G (2/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

CGCCGGACTGGCGGGTCGGCAGGCGCAGCATGGCCATTACCGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGTCGGCGATTACCAGGATGC  >  NC_002947/2658245‑2658520
                                                                                                                                        |                                                                                                                                           
cgccgGACTGGCGGGTCGGCAGGCGCAGCATGGCCATTACCGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAAGATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGcgcggtcgcgg                                                                                                                                 >  7:238057/1‑149 (MQ=255)
 gccgGACTGGCGGGTCGGCAGGCGCAGCATGGCCATTACCGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGcgcggtcgcggt                                                                                                                                <  2:45966/149‑1 (MQ=255)
          gCGGGTCGGCAGGCGCAGCATGGCCATTACCGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCccgcc                                                                                                                         >  8:13432/1‑147 (MQ=255)
                   cAGGCGCAGCATGGCCATTACCGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTa                                                                                                                >  7:161793/1‑147 (MQ=255)
                         cagcaTGGCCATTACCGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAg                                                                                                        <  7:13432/149‑1 (MQ=255)
                               ggCCATTACCGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGa                                                                                                  >  3:337999/1‑149 (MQ=255)
                                  cATTACCGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGcc                                                                                                           <  2:2657/137‑1 (MQ=255)
                                  cATTACCGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAgattcgg                                                                                                  >  1:2657/1‑140 (MQ=255)
                                        cGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGGGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGAt                                                                                                 <  2:171231/141‑1 (MQ=255)
                                        cGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGGGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGAt                                                                                                 <  2:321857/141‑1 (MQ=255)
                                        cGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGGGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGAt                                                                                                 >  1:321857/1‑141 (MQ=255)
                                        cGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGGGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGAt                                                                                                 >  1:171231/1‑141 (MQ=255)
                                                ccATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTg                                                                                             <  6:296826/137‑1 (MQ=255)
                                                ccATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTg                                                                                             >  5:296826/1‑137 (MQ=255)
                                                 cATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCt                                                                                >  3:313483/1‑149 (MQ=255)
                                                      gCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTgg                                                                                    <  7:120306/140‑1 (MQ=255)
                                                      gCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTgg                                                                                    >  8:120306/1‑140 (MQ=255)
                                                       cACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGa                                                                                           >  4:316210/1‑132 (MQ=255)
                                                       cACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGa                                                                                           <  3:316210/132‑1 (MQ=255)
                                                               tgCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATgg                                                                  >  8:276791/1‑149 (MQ=255)
                                                               tgCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATgg                                                                  >  7:274548/1‑149 (MQ=255)
                                                                 cGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCt                                                                <  5:187862/149‑1 (MQ=255)
                                                                   gATGTCCGTTACTCCTTCGTATCTGATGCTCTTGAATTTCTGGTCGGGGGTCACGCGGTACTCGATCAGCGGGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCCCATGGCtga                                                              <  8:161793/149‑1 (MQ=255)
                                                                   gATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCtga                                                              <  7:319780/149‑1 (MQ=255)
                                                                            tACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGc                                                     >  2:30059/1‑149 (MQ=255)
                                                                              cacCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGcc                                                    <  4:313483/148‑1 (MQ=255)
                                                                               acCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGccc                                                   >  5:140840/1‑148 (MQ=255)
                                                                                 cTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAggg                                                                                                                    >  8:126958/1‑81 (MQ=255)
                                                                                 cTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAggg                                                                                                                    <  7:126958/81‑1 (MQ=255)
                                                                                  ttCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGa                                                 >  7:230255/1‑147 (MQ=255)
                                                                                     gTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAgg                                                                                                                     <  3:43884/76‑1 (MQ=255)
                                                                                     gTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAgg                                                                                                                     >  4:43884/1‑76 (MQ=255)
                                                                                                tctTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACtgtg                                          <  8:19032/140‑1 (MQ=255)
                                                                                                tctTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACtgtg                                          >  7:19032/1‑140 (MQ=255)
                                                                                                tctTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGa                                 >  6:363015/1‑149 (MQ=255)
                                                                                                 ctTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCGGTGAGGATGCTGGAAATCTGGTGCTCGAATTCCTCATCGCTGATGATCTTGCCAGACACTGTGCGGTAGCGAt                                >  8:123354/1‑149 (MQ=255)
                                                                                                  ttGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCACATGGCTGATGCTCTTGCCCGACACTGTGCGGTa                                     >  3:267799/1‑143 (MQ=255)
                                                                                                    gAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGaa                                                                                          <  4:316955/88‑1 (MQ=255)
                                                                                                    gAATATCTGGTCCGGGGTAACGCGGTACTAGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGaa                                                                                          >  3:316955/1‑88 (MQ=255)
                                                                                                     aaTATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCt                             <  8:230255/148‑1 (MQ=255)
                                                                                                      atatCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTc                            >  4:68182/1‑148 (MQ=255)
                                                                                                       tatCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGc                                         <  3:340554/134‑1 (MQ=255)
                                                                                                       tatCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGc                                         >  4:340554/1‑134 (MQ=255)
                                                                                                         tCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAg                        >  1:287059/1‑149 (MQ=255)
                                                                                                            ggTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCg                      <  2:348935/148‑1 (MQ=255)
                                                                                                            ggTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCg                      >  1:348935/1‑148 (MQ=255)
                                                                                                             gTCCGGGGTCACGCTGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAg                        <  1:299674/145‑1 (MQ=255)
                                                                                                             gTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCGCAGTGAGGATGCTGGATATCTGGTGCTCGATTTCCTCATTGCTGATGATCTTGGGCTAGACTGTGCGGTAGCGATCCTCGAAGCg                      <  4:337999/147‑1 (MQ=255)
                                                                                                             gTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAg                        <  5:363015/145‑1 (MQ=255)
                                                                                                             gTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGt                    >  7:100555/1‑149 (MQ=255)
                                                                                                                     tCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGTCGGCGAt             >  2:99103/1‑148 (MQ=255)
                                                                                                                       aCGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGTCGGCGATTa           <  3:68182/148‑1 (MQ=255)
                                                                                                                         gcgGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGTCGGCGATTAc          >  3:51150/1‑147 (MQ=255)
                                                                                                                           ggTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGTCGGCGATTACCAgg      <  1:99103/149‑1 (MQ=255)
                                                                                                                              aCTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGTCGGCGATTACCAGGATg   >  1:273237/1‑149 (MQ=255)
                                                                                                                               cTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGTCGGCGATTACCAGGATGc  <  1:30059/149‑1 (MQ=255)
                                                                                                                                   aTCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCgg                     >  5:144423/1‑126 (MQ=255)
                                                                                                                                   aTCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCgg                     <  6:144423/126‑1 (MQ=255)
                                                                                                                                        cgcgcgGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGTCGGCg               >  2:27757/1‑127 (MQ=255)
                                                                                                                                        cgcgcgGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGTCGGCg               <  1:27757/127‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
CGCCGGACTGGCGGGTCGGCAGGCGCAGCATGGCCATTACCGGGTAGCCCATCAGCACGATGATGCGGATGTCCGGTACACCTTCGTAGCTGATGCTCTTGAATATCTGGTCCGGGGTCACGCGGTACTCGATCAGCGCGCGGTCGCGGTGCCCGCCGAGGGAGTACAGCCCAGTGAGGATGCTGGAAATCTGGTGCTCGATTTCCTCATGGCTGATGATCTTGCCCGACACTGTGCGGTAGCGATCCTCGAAGCGGTCGGCGATTACCAGGATGC  >  NC_002947/2658245‑2658520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑