Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,670,284	G→A	9.3%	A678A (GCC→GCT)	acnB ←	aconitate hydratase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,670,284	0	G	A	9.3%	116.4 / 2.4	43	A678A (GCC→GCT)	acnB	aconitate hydratase B
Reads supporting (aligned to +/- strand):  ref base G (20/19);  new base A (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01

CCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAG  >  NC_002947/2670136‑2670430                                                                                                                                                     |                                                                                                                                                    ccAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCg                                                                                                                                                     >  7:36849/1‑149 (MQ=255)  cagtagCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCg                                                                                                                                                     <  7:167601/148‑1 (MQ=255)  cagcagCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCg                                                                                                                                                     <  2:58895/148‑1 (MQ=255)   agcagcTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGc                                                                                                                                                   <  5:322688/149‑1 (MQ=255)   agcagcTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGc                                                                                                                                                   >  6:322688/1‑149 (MQ=255)      agcTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAg                                                                                                                                                <  3:310108/149‑1 (MQ=255)                    gcgGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGT‑AGCCAGGTCGATTTCgat                                                                                                                                    <  5:327078/146‑1 (MQ=255)                    gcgGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGT‑AGCCAGGTCGATTTCgat                                                                                                                                    >  6:327078/1‑146 (MQ=255)                        aaGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTc                                                                                                                              >  3:48099/1‑149 (MQ=255)                                gATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTAtt                                                                                                                      >  4:197420/1‑149 (MQ=255)                                  tGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCg                                                                                                                    <  2:83245/149‑1 (MQ=255)                                  tGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCg                                                                                                                    <  3:197420/149‑1 (MQ=255)                                        tCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGc                                                                                                                           <  2:263435/136‑1 (MQ=255)                                        tCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGc                                                                                                                           >  1:263435/1‑136 (MQ=255)                                         cATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCgg                                                                                                             >  4:335536/1‑149 (MQ=255)                                             cACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGt                                                                                                          <  5:65953/148‑1 (MQ=255)                                              aCGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGt                                                                                                                         <  7:216326/132‑1 (MQ=255)                                              aCGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGt                                                                                                                         >  8:216326/1‑132 (MQ=255)                                                                 gtcgATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGACGACAGCAGCTCAGGCt                                                                                     >  6:190715/1‑149 (MQ=255)                                                                    gATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCAGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTgg                                                                                  >  3:106122/1‑149 (MQ=255)                                                                             gCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCGGCTCAGGCTTGgccagcca                                                                           >  3:50911/1‑147 (MQ=255)                                                                             gCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCGGCTCAGGCTTGgccagcca                                                                           <  4:50911/147‑1 (MQ=255)                                                                                ttGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGcca                                                                      >  5:346418/1‑147 (MQ=255)                                                                                   tACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTcc                                                                    <  8:36849/148‑1 (MQ=255)                                                                                   tACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCa                                                                   <  3:335536/149‑1 (MQ=255)                                                                                                agGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGc                                                                                                                           <  6:346213/80‑1 (MQ=255)                                                                                                agGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGc                                                                                                                           >  5:346213/1‑80 (MQ=255)                                                                                                    gggCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCt                                                             <  1:148452/138‑1 (MQ=255)                                                                                                    gggCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCt                                                             >  2:148452/1‑138 (MQ=255)                                                                                                       cATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTcc                                              >  8:116920/1‑149 (MQ=255)                                                                                                         tCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAg                                            <  4:310250/149‑1 (MQ=255)                                                                                                           gTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAgg                                           <  4:48099/148‑1 (MQ=255)                                                                                                              cGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCAGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGc                                       <  4:106122/149‑1 (MQ=255)                                                                                                                ggTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGAca                                                                                                 >  4:115461/1‑90 (MQ=255)                                                                                                                ggTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGAca                                                                                                 <  3:115461/90‑1 (MQ=255)                                                                                                                ggTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCgg                                     <  7:116920/149‑1 (MQ=255)                                                                                                                ggTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCgg                                     <  8:80673/149‑1 (MQ=255)                                                                                                                 gTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCggg                                    >  4:165141/1‑149 (MQ=255)                                                                                                                               agagCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCt                      >  6:159466/1‑149 (MQ=255)                                                                                                                                   cacaGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGAGCGCCGCGC‑CCAggccat                                       <  5:343756/128‑5 (MQ=255)                                                                                                                                   cacaGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGGAGCGCCGCGC‑CCAggccat                                       >  6:343756/1‑124 (MQ=255)                                                                                                                                    acaGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGcc                 >  2:131276/1‑149 (MQ=255)                                                                                                                                      aGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCCGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGcc                 >  7:164059/1‑147 (MQ=255)                                                                                                                                      aGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGc                  >  4:238593/1‑146 (MQ=255)                                                                                                                                      aGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGc                  <  3:238593/146‑1 (MQ=255)                                                                                                                                                   tCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCgcag  >  1:63585/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CCAGCAGCTTGCCGGCAGCGCGGAAGTGACCGATGTTGGTCATGCACGAACCGATGAACACTTCGTCGATCTTCTCGCCTTGTACCGAGGACAGCAGGCGGGCATCGTCCGGGTCGTTCGGCGCGCAGAGCACAGGCTCCTTGACGTCGGCCAGGTCGATTTCGATGATTTCGGCGTATTCGGCATCGGCGTCGGCCGACAGCAGCTCAGGCTTGGCCAGCCAGGCTTCCATGGCCTGG‑GCGCGGCGCTCCAGGGTGCGGGCATCGCCGTAGCCTTCGCCGATCATCCAGCGCAG  >  NC_002947/2670136‑2670430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑