Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,687,305 C→T 7.7% A139A (GCG→GCA)  PP_2354 ← two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,687,3050CT 7.7% 147.0 / 2.0 53A139A (GCG→GCA) PP_2354two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (28/20);  new base T (2/2);  total (31/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

ACACTTCAAGGCTCATTTCGGTGCTGGTGGGCACGCCGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAGTCGGTCGCGCCCAGGCGGATCATTTCCACCGCGTGCTCTTCGC  >  NC_002947/2687157‑2687453
                                                                                                                                                     |                                                                                                                                                     
acacTTCAAGGCTCATTTCGGTGCTGGTGGGCACGCCGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTcc                                                                                                                                                       <  5:204350/149‑1 (MQ=255)
    ttCAAGGCTCATTTCGGTGCTGGTGGGCACGCCGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGcc                                                                                                                                                    >  6:84358/1‑148 (MQ=255)
       aaGGCTCATTTCGGTGCTGGTGGGCACGCCGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCAGCCAAgg                                                                                                                                                >  4:312233/1‑149 (MQ=255)
          gCTCATTTCGGTGCTGGTGGGCACGCCGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTcc                                                                                                                                                       <  2:341784/139‑1 (MQ=255)
          gCTCATTTCGGTGCTGGTGGGCACGCCGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTcc                                                                                                                                                       >  1:341784/1‑139 (MQ=255)
           cTCATTTCGGTGCTGGTGGGCACGCCGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCctc                                                                                                                                            >  5:160435/1‑149 (MQ=255)
                  cGGTGCTGGTGGGCACGCCGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTcggcg                                                                                                                                     >  3:93641/1‑149 (MQ=255)
                              gCACGCCGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGgcgc                                                                                                                         >  6:107771/1‑149 (MQ=255)
                                    cGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGGTGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTg                                                                                                                       >  1:41009/1‑145 (MQ=255)
                                    cGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGGTGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTg                                                                                                                       <  2:41009/145‑1 (MQ=255)
                                       aCAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTcc                                                                                                                <  5:84358/149‑1 (MQ=255)
                                        cAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGTTCCCAGGTGCCCATGCAGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCt                                                                                                               <  6:172766/149‑1 (MQ=255)
                                                     cGGTCATCCCACAGCAACAGGCCCTCTTGCGGGCGGCAGTTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACTTCCGCCAAGGCATCTTCGCCGCGCCGACGGCGCTGGCGTTCCT‑GTACTTCgat                                                                                                    >  1:52839/1‑145 (MQ=255)
                                                         cATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCt                                                                                                               <  8:168664/132‑1 (MQ=255)
                                                         cATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCt                                                                                                               >  7:168664/1‑132 (MQ=255)
                                                            cccACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTc                                                                                                                 >  6:124486/1‑127 (MQ=255)
                                                            cccACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTc                                                                                                                 <  5:124486/127‑1 (MQ=255)
                                                              cacaGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGAGCTGGCGTTCCT‑GTAATTCGCTCAGGGCccgccg                                                                                        >  6:231508/1‑149 (MQ=255)
                                                               acaGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCTGCCAAGGCctct                                                                                                                                           >  1:313170/1‑98 (MQ=255)
                                                               acaGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCTGCCAAGGCctct                                                                                                                                           <  2:308910/98‑1 (MQ=255)
                                                               acaGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCTGCCAAGGCctct                                                                                                                                           <  2:313170/98‑1 (MQ=255)
                                                               acaGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCTGCCAAGGCctct                                                                                                                                           >  1:308910/1‑98 (MQ=255)
                                                               acaGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGgcgc                                                                                       <  5:107771/149‑1 (MQ=255)
                                                                        ggCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTg                                                                              <  4:93641/149‑1 (MQ=255)
                                                                         gCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCt                                                                                                               >  8:9380/1‑116 (MQ=255)
                                                                         gCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCt                                                                                                               <  7:9380/116‑1 (MQ=255)
                                                                                   gggC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGTGCAGCAGTgg                                                                   <  6:160435/149‑1 (MQ=255)
                                                                                        gcAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCTAGGCCTAATAGGCGCGGCCACGGCGCTGGCGTTACT‑GTACGTAGCTCAGGGCCCGGCGCACGGCGTTGGGCAGCCGTGGCatg                                                               >  1:278785/1‑147 (MQ=255)
                                                                                        gcAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCt                                                                                 >  1:210990/1‑131 (MQ=255)
                                                                                        gcAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCt                                                                                 <  2:210990/131‑1 (MQ=255)
                                                                                        gcAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAgg                                                               <  2:235462/149‑1 (MQ=255)
                                                                                          aGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGtt                                                             >  5:159816/1‑149 (MQ=255)
                                                                                              cccAGGTGCCCATTGCGGCGGCGTCGATGGCGATGGGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGGGGCGACGGCTCTGGCGTTCGT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGt                                                                     <  4:309572/137‑1 (MQ=255)
                                                                                              cccAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGt                                                                     >  3:309572/1‑137 (MQ=255)
                                                                                                 aGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGCCGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGttcttctt                                                       >  5:79328/1‑148 (MQ=255)
                                                                                                 aGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTcc                                                                                                                >  8:239925/1‑92 (MQ=255)
                                                                                                 aGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTcc                                                                                                                <  7:239925/92‑1 (MQ=255)
                                                                                                 aGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGttcttcttc                                                      <  3:312233/149‑1 (MQ=255)
                                                                                                       ccATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGcaca                                                >  3:239380/1‑149 (MQ=255)
                                                                                                       ccATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGcaca                                                >  2:262079/1‑149 (MQ=255)
                                                                                                       ccATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGcac                                                 >  3:97887/1‑148 (MQ=255)
                                                                                                          tGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAg                                             <  5:231508/149‑1 (MQ=255)
                                                                                                            ccGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAgtc                                           <  6:159816/149‑1 (MQ=255)
                                                                                                             cggcggCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGCCGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAgtcg                                          >  8:170337/1‑149 (MQ=255)
                                                                                                                   cgTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTa                                                                                                           >  2:290231/1‑78 (MQ=255)
                                                                                                                   cgTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTa                                                                                                           <  1:290231/78‑1 (MQ=255)
                                                                                                                    gTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAGTCGGTCgcgc                                   <  5:180470/149‑1 (MQ=255)
                                                                                                                           gCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGgc                                                                           >  8:315699/1‑102 (MQ=255)
                                                                                                                           gCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGgc                                                                           <  7:315699/102‑1 (MQ=255)
                                                                                                                             gATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTTGGCAGCAGTGGCAGGttct                                                           <  6:121541/116‑1 (MQ=255)
                                                                                                                             gATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGttct                                                           >  5:121541/1‑116 (MQ=255)
                                                                                                                             gATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAGTCGGTCGCGCCCAGGCGGa                          >  2:257255/1‑149 (MQ=255)
                                                                                                                                     cACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAGTCGGTCGCGCCCAGGCGGATCATTTc                   >  1:204554/1‑148 (MQ=255)
                                                                                                                                            ttCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCTGGTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAGTCGGTCGCGCCCAGGCGGATCATTTCCACcgcg            >  1:261458/1‑149 (MQ=255)
                                                                                                                                            ttCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAGTCGGTCGCGCCCAGGCGGATCATTTCCACCGCGt           >  2:192690/1‑149 (MQ=255)
                                                                                                                                                aCGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAGTCGGTCGCGCCCAGGCGGATCATTTCCACCGCGTGctc       >  5:131155/1‑149 (MQ=255)
                                                                                                                                                    ccGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAGTCGGTCGCGCCCAGGCGGATCATTTCCACCGCGTGCTCTTCg   >  5:157838/1‑149 (MQ=255)
                                                                                                                                                     cGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAGTCGGTCGCGCCCAGGCGGATCATTTCCACCGCGTGCTCTTCGc  <  4:239380/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACACTTCAAGGCTCATTTCGGTGCTGGTGGGCACGCCGAACAAGGTCTTGCAGCGGTCATCCCACAGCAACAGGCCCTCTTGCGGGC‑GCAGGTCCCAGGTGCCCATGCCGGCGGCGTCGATGGCGATGCGGGCACGTGCTTCGACGTCCGCCAAGGCCTCTTCGGCGCGGCGACGGCGCTGGCGTTCCT‑GTACTTCGCTCAGGGCCCGGCGCACGGCTTTGGGCAGCAGTGGCAGGTTCTTCTTCAGCACATAGTCGGTCGCGCCCAGGCGGATCATTTCCACCGCGTGCTCTTCGC  >  NC_002947/2687157‑2687453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑