Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,693,678 C→G 8.0% A259G (GCC→GGC)  PP_2361 → chaperone protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,693,6780CG 8.0% 155.1 / 3.6 50A259G (GCC→GGC) PP_2361chaperone protein
Reads supporting (aligned to +/- strand):  ref base C (25/21);  new base G (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

AAGGCAGCCAGAGCAAGCCACTGGCTGAAGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGC  >  NC_002947/2693537‑2693821
                                                                                                                                             |                                                                                                                                               
aaGGCAGCCAGAGCAAGCCACTGGCTGAAGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGc                                                                                                                                          <  3:86514/149‑1 (MQ=255)
      gCCAGAGCAAGCCACTGGCTGAAGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGc                                                                                                                                    <  7:200604/149‑1 (MQ=255)
       ccAGAGCAAGCCACTGGCTGAAGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGc                                                                                                                                    >  8:329215/1‑148 (MQ=255)
             cAAGCCACTGGCTGAAGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAg                                                                                                                             <  7:24104/149‑1 (MQ=255)
              aaGCCACTGGCTGAAGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGATGGCGTCCGccga                                                                                                                                       >  1:159754/1‑136 (MQ=255)
              aaGCCACTGGCTGAAGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGATGGCGTCCGccga                                                                                                                                       <  2:159754/138‑3 (MQ=255)
                  cACTGGCTGAAGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAAc                                                                                                                        >  2:77291/1‑149 (MQ=255)
                          gAAGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAAt                                                                                                                >  1:189263/1‑149 (MQ=255)
                           aaGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAAt                                                                                                                >  3:190455/1‑148 (MQ=255)
                            aGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATg                                                                                                               <  4:190455/148‑1 (MQ=255)
                             ggCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGaa                                                                                                             <  2:189263/149‑1 (MQ=255)
                              gCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGTTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAg                                                                                                            >  7:185750/1‑149 (MQ=255)
                                   ctgGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAg                                                                                                       <  1:77291/149‑1 (MQ=255)
                                          aTGTGCTGCCGGGGGCAATCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCa                                                                                                 <  7:97740/148‑1 (MQ=255)
                                          aTGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCa                                                                                                 <  8:185750/148‑1 (MQ=255)
                                               ctgcC‑GGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGggagga                                                                                          <  4:217631/149‑1 (MQ=255)
                                               ctgcC‑GGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGggagga                                                                                          <  4:222170/149‑1 (MQ=255)
                                                     ggggCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCgg                                                                                     >  6:61172/1‑149 (MQ=255)
                                                          aaGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATg                                                                                >  5:423/1‑149 (MQ=255)
                                                          aaGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATg                                                                                >  5:247136/1‑149 (MQ=255)
                                                              aTGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGCCCCGGCAATGGTTg                                                                            >  1:308600/1‑149 (MQ=255)
                                                              aTGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTg                                                                            >  7:96478/1‑149 (MQ=255)
                                                                      gCCGGCTCCCGTGGCATCCGGCAACTGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCg                                                                     <  5:61172/148‑1 (MQ=255)
                                                                      gCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAAcc                                                                      <  6:423/147‑1 (MQ=255)
                                                                         ggCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTg                                                                            >  6:251010/1‑138 (MQ=255)
                                                                         ggCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTg                                                                            <  5:251010/138‑1 (MQ=255)
                                                                          gCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCgtgtgt                                                                >  5:337231/1‑149 (MQ=255)
                                                                              ccGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAgg                                                                                                                                     >  2:345275/1‑76 (MQ=255)
                                                                              ccGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAgg                                                                                                                                     <  1:345275/76‑1 (MQ=255)
                                                                              ccGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGaaa                                                            >  4:115229/1‑149 (MQ=255)
                                                                                     aTCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTcc                                                     >  1:106991/1‑149 (MQ=255)
                                                                                      tCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGt                                                                              <  2:93366/123‑1 (MQ=255)
                                                                                      tCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGt                                                                              >  1:93366/1‑123 (MQ=255)
                                                                                      tCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTccc                                                    <  8:96478/149‑1 (MQ=255)
                                                                                           cAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGcga                                               <  6:337231/149‑1 (MQ=255)
                                                                                               ggCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCCATGGTTGGAAACCGTGTGGGACATGGCTCCCGGCgccgac                                           >  4:262882/1‑149 (MQ=255)
                                                                                                        cTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGCCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCACCCGGCGACGACGTGCCGCTg                                  >  5:118996/1‑149 (MQ=255)
                                                                                                         tCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTgg                                 >  7:34714/1‑149 (MQ=255)
                                                                                                              ggCAGTGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCt                            <  6:247136/149‑1 (MQ=255)
                                                                                                              ggCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCt                            >  1:326616/1‑149 (MQ=255)
                                                                                                                cAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTgg                          <  2:326616/149‑1 (MQ=255)
                                                                                                                     tCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTg                     >  2:217876/1‑149 (MQ=255)
                                                                                                                            tCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATgg              <  7:329215/149‑1 (MQ=255)
                                                                                                                              aaCACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGt             >  4:296959/1‑148 (MQ=255)
                                                                                                                                cacTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAg          >  5:233234/1‑149 (MQ=255)
                                                                                                                                   ttggcggATGGCGTCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGtt                                                                             <  6:123019/77‑1 (MQ=255)
                                                                                                                                   ttggcggATGGCGTCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGtt                                                                             >  5:123019/3‑79 (MQ=255)
                                                                                                                                    cggcggACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGc                              <  7:202852/125‑1 (MQ=255)
                                                                                                                                    cggcggACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGc                              >  8:202852/1‑125 (MQ=255)
                                                                                                                                        ggACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGCACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCgcgc  >  7:244949/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                               
AAGGCAGCCAGAGCAAGCCACTGGCTGAAGGCCTGCTGGGTTATGTGCTGCCGGGGGCAAGCATGCGCTGGCCGGCTCCCGTGGCATCCGGCAACGGCAGCGTGCTCAAGGGCAGGGTCAATGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGC  >  NC_002947/2693537‑2693821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑