Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	284,586	T→A	10.8%	intergenic (+33/+30)	betT‑II → / ← atsK	choline/carnitine/betaine transporter/alpha‑ketoglutarate‑dependent sulfonate dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	284,586	0	T	A	10.8%	157.4 / 6.0	61	intergenic (+33/+30)	betT‑II/atsK	choline/carnitine/betaine transporter/alpha‑ketoglutarate‑dependent sulfonate dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (30/24);  new base A (5/2);  total (35/26)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTGAGCGAAGGCAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCACGTCATTCTCC  >  NC_002947/284438‑284733                                                                                                                                                     |                                                                                                                                                    aCCTGAGCGAAGGCAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCt                                                                                                                                                     >  4:143242/1‑149 (MQ=255)     gAGCGAAGGCAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGcc                                                                                                                                                 >  2:111274/1‑149 (MQ=255)      aGCGAAGGCAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGc                                                                                                                                                  <  3:143242/147‑1 (MQ=255)               aGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCAAGCCCGCGAAGAgg                                                                                                                                       >  6:251912/1‑149 (MQ=255)                gCCAGAACTATGACCTGGTGGGCTACAGCAAGCAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCTCGGGCTTGCCCGCGAAGAGGc                                                                                                                                      <  4:271090/149‑1 (MQ=255)                  cAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGc                                                                                                                                      >  4:96895/1‑147 (MQ=255)                  cAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCa                                                                                                                                    <  5:128612/149‑1 (MQ=255)                   aGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCAAGCCCGCGAAGAGGCCAg                                                                                                                                   <  5:251912/149‑1 (MQ=255)                                        aCAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGt                                                                                                              <  1:111274/149‑1 (MQ=255)                                         cAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAg                                                                                                                >  4:245772/1‑146 (MQ=255)                                         cAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAg                                                                                                                <  3:245772/146‑1 (MQ=255)                                            cAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGTAAGGt                                                                                                              <  7:320203/145‑1 (MQ=255)                                            cAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGt                                                                                                              >  8:320203/1‑145 (MQ=255)                                               ggAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATccc                                                                                                       >  4:123206/1‑149 (MQ=255)                                               ggAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATcc                                                                                                        >  3:272250/1‑148 (MQ=255)                                                      atcatcAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAg                                                                                                                    <  3:264887/129‑1 (MQ=255)                                                      atcatcAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAg                                                                                                                    >  4:264887/1‑129 (MQ=255)                                                            aaCGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGc                                                                                          <  3:96895/149‑1 (MQ=255)                                                                          ccAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGc                                                                                   <  6:136689/142‑1 (MQ=255)                                                                          ccAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGc                                                                                   >  5:136689/1‑142 (MQ=255)                                                                           cAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACccg                                                                           >  5:73495/1‑149 (MQ=255)                                                                           cAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACccg                                                                           >  1:3139/1‑149 (MQ=255)                                                                                     ggCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTtcggt                                                                 <  2:3139/149‑1 (MQ=255)                                                                                        acaTGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTtcggtcgg                                                              >  2:20842/1‑149 (MQ=255)                                                                                           tGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTaa                                                           <  3:123206/149‑1 (MQ=255)                                                                                               gTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCgg                                                                                      <  4:205722/118‑1 (MQ=255)                                                                                               gTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCgg                                                                                      >  3:205722/1‑118 (MQ=255)                                                                                                  ccTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTtcg                                                                   <  3:96858/134‑1 (MQ=255)                                                                                                  ccTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTtcg                                                                   >  4:96858/1‑134 (MQ=255)                                                                                                  ccTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAat                                                          <  4:145771/143‑1 (MQ=255)                                                                                                  ccTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAat                                                          >  3:145771/1‑143 (MQ=255)                                                                                                  ccTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTAGCGGGCTTGCCCGCGAAGAGGCCCGCCAGGCAACCGCGAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGGGCATCACCCGCCGGGTGGGTCGGGAATGATCCa                                                    >  5:50515/1‑149 (MQ=255)                                                                                                     gagcttGGTCAGCGGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTtcggt                                                                 <  2:19751/128‑1 (MQ=255)                                                                                                     gagcttGGTCAGCGGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTtcggt                                                                 >  1:19751/6‑133 (MQ=255)                                                                                                     gCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCAc                                                   <  7:201751/147‑1 (MQ=255)                                                                                                     gCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCAc                                                   >  8:201751/1‑147 (MQ=255)                                                                                                        ccTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCa                                               >  7:311757/1‑148 (MQ=255)                                                                                                               cGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGGTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       <  8:299228/149‑1 (MQ=255)                                                                                                               cGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       <  6:73495/149‑1 (MQ=255)                                                                                                               cGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       <  1:20842/149‑1 (MQ=255)                                                                                                               cGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       >  2:27239/1‑149 (MQ=255)                                                                                                                     ccAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCAc                                 >  6:161357/1‑149 (MQ=255)                                                                                                                       aaGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCAcgc                               >  3:85262/1‑149 (MQ=255)                                                                                                                        aGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCAcgcg                              >  5:99436/1‑149 (MQ=255)                                                                                                                         gCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCgg                             <  5:161357/149‑1 (MQ=255)                                                                                                                             tcctgtcctgGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGAt                                                                                                >  8:214865/1‑78 (MQ=255)                                                                                                                             tcctgtcctgGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGt                         >  1:300518/1‑149 (MQ=255)                                                                                                                                  tcctgGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAg                    <  1:27239/149‑1 (MQ=255)                                                                                                                                    ctgGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGaa                  >  2:87533/1‑149 (MQ=255)                                                                                                                                       gCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCTGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGc               >  7:184983/1‑149 (MQ=255)                                                                                                                                       gCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCTGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGc               <  8:184983/149‑1 (MQ=255)                                                                                                                                       gCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTAGGTAGGTAATCATCCACCGCAAAAAGcg                                        >  8:283933/1‑123 (MQ=255)                                                                                                                                       gCCTCTTCGCGGGCAAGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGc               >  7:249958/1‑149 (MQ=255)                                                                                                                                         ctctTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAatc                                                         <  4:81288/105‑1 (MQ=255)                                                                                                                                         ctctTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAatc                                                         >  3:81288/1‑105 (MQ=255)                                                                                                                                         ctctTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCa             >  3:309836/1‑149 (MQ=255)                                                                                                                                         ctctTCGCGGGCAAGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCa             >  8:94149/1‑149 (MQ=255)                                                                                                                                          tctTCGCGGGCAAGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       <  5:223495/122‑1 (MQ=255)                                                                                                                                          tctTCGCGGGCAAGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCAGACGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       >  6:223495/1‑122 (MQ=255)                                                                                                                                            ttCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGc                                                <  4:198443/111‑1 (MQ=255)                                                                                                                                            ttCGCGGGCAAGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCAAGAACGCCACGt          >  8:49973/1‑149 (MQ=255)                                                                                                                                                    cTTGCCCGCGAAGAGGCCAGACAGGCAAGCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCAACACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATCCTGGGTCACGCGGTTGTCCCAGAACGCCACGTCATTCTcc  >  1:196770/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ACCTGAGCGAAGGCAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGTCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACCGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCACGTCATTCTCC  >  NC_002947/284438‑284733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑