Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,738,424 C→G 10.0% T268R (ACG→AGG)  PP_2395 → leucine‑rich repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,738,4240CG10.0% 120.4 / 4.1 40T268R (ACG→AGG) PP_2395leucine‑rich repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (20/16);  new base G (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

ATGGTGGCTGTGTGCGAGAAGGGGGAGCTGCCGCGCCTGGCGGGCGACCTTGTGCTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCAGCCGCTTCGTGCTGCGGC  >  NC_002947/2738285‑2738570
                                                                                                                                           |                                                                                                                                                   
aTGGTGGCTGTGTGCGAGAAGGGGGAGCTGCCGCGCCTGGCGGGCGACCTTGTGCTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACacgac                                                                                                                                                  <  7:188758/143‑1 (MQ=255)
aTGGTGGCTGTGTGCGAGAAGGGGGAGCTGCCGCGCCTGGCGGGCGACCTTGTGCTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACacgac                                                                                                                                                  >  8:188758/1‑143 (MQ=255)
          tgtgCGAGAAGGGGGAGCTGCCGCGCCTGGCGGGCGACCTTGTGCTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCtt                                                                                                                                  <  8:158928/149‑1 (MQ=255)
                   aGGGGGAGCTGCCGCGCCTGGCGGGCGACCTTGTGCTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCt                                                                                                                         <  8:253316/149‑1 (MQ=255)
                     ggggAGCTGCCGCGCCTGGCGGGCGACCTTGTGCTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTgg                                                                                                                       <  2:59540/149‑1 (MQ=255)
                                                 ttGTGCTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTc                                                                                          >  5:225299/1‑149 (MQ=255)
                                                     gCTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGCGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGgcgc                                                                                                >  7:278467/1‑139 (MQ=255)
                                                      cTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGagag                                                                                     >  2:31556/1‑149 (MQ=255)
                                                      cTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCCTTT‑TCGcc                                                                                                       >  4:55077/1‑131 (MQ=255)
                                                      cTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCCTTT‑TCGcc                                                                                                       <  3:55077/131‑1 (MQ=255)
                                                              gCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTTCTCGCCCTGGCGCGCGTTCGAGAGCGTCGAc                                                                              <  1:31556/149‑1 (MQ=255)
                                                                              ggTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAAc                                                              <  6:225299/148‑1 (MQ=255)
                                                                                   ccAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGt                                                                                            >  2:63899/1‑113 (MQ=255)
                                                                                   ccAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGt                                                                                            <  1:63899/113‑1 (MQ=255)
                                                                                                   aCCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGAc                                          >  3:63711/1‑147 (MQ=255)
                                                                                                   aCCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGAc                                          >  1:169698/1‑147 (MQ=255)
                                                                                                   aCCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACaa                                        <  2:169698/149‑1 (MQ=255)
                                                                                                   aCCGTGTTCATTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGTGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGAc                                          >  5:119950/1‑147 (MQ=255)
                                                                                                    ccGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAg                                       >  6:254407/1‑149 (MQ=255)
                                                                                                     cGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGACCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAgg                                      >  5:223306/1‑149 (MQ=255)
                                                                                                            gTTGTCATTGATCAGGTCGTCCTGTTCATCACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCa                               <  8:86617/149‑1 (MQ=255)
                                                                                                              tGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCAGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGAGAGACAAGGCCTATCAGc                             >  5:353761/1‑149 (MQ=255)
                                                                                                                tCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCtt                                                                                                                                  >  8:293390/1‑47 (MQ=255)
                                                                                                                tCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCtt                                                                                                                                  <  7:293390/47‑1 (MQ=255)
                                                                                                                  aTTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCg                                                       >  4:288229/1‑119 (MQ=255)
                                                                                                                  aTTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCg                                                       <  3:288229/119‑1 (MQ=255)
                                                                                                                   ttGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGTCAAACTGAACCGCGAACTGGGCCGTTGCTTGCga                                             >  8:54671/1‑128 (MQ=255)
                                                                                                                   ttGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGTCAAACTGAACCGCGAACTGGGCCGTTGCTTGCga                                             <  7:54671/128‑1 (MQ=255)
                                                                                                                   ttGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTttc                        >  2:104869/1‑149 (MQ=255)
                                                                                                                     gAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTttctt                      >  3:262345/1‑149 (MQ=255)
                                                                                                                       gCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCa                    <  4:63711/149‑1 (MQ=255)
                                                                                                                         gccgCGTCGTGTTGAACAGGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCga                                             <  4:175639/119‑1 (MQ=255)
                                                                                                                         gccgCGTCGTGTTGAACAGGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCga                                             >  3:175639/4‑122 (MQ=255)
                                                                                                                             cgtcgtGTTGAACAGGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGttgcttgc                                               <  6:12967/116‑1 (MQ=255)
                                                                                                                             cgtcgtGTTGAACAGGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGttgcttgc                                               >  5:12967/1‑116 (MQ=255)
                                                                                                                               tCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCAGCCGCTTc            <  1:104869/149‑1 (MQ=255)
                                                                                                                                 cTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGttgcttgc                                               >  3:94576/1‑112 (MQ=255)
                                                                                                                                 cTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGttgcttgc                                               <  4:94576/112‑1 (MQ=255)
                                                                                                                                   gTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCAGCCGCTTCGtgc        >  6:337662/1‑149 (MQ=255)
                                                                                                                                     tCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCAGc                  >  6:234160/1‑137 (MQ=255)
                                                                                                                                     tCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCAGc                  <  5:234160/137‑1 (MQ=255)
                                                                                                                                       aaCACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCAGCCGCTTCGTGCTgcg    >  7:3029/1‑149 (MQ=255)
                                                                                                                                         cacGACGCGGTGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCAGCCGCTTCGTGCTgcggc  <  6:119950/149‑1 (MQ=255)
                                                                                                                                         cacGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCa                    <  2:279735/131‑1 (MQ=255)
                                                                                                                                         cacGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCa                    >  1:279735/1‑131 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
ATGGTGGCTGTGTGCGAGAAGGGGGAGCTGCCGCGCCTGGCGGGCGACCTTGTGCTGGCCAGGCAACTGAAATTGCTGGGTTGCCAGATTGAGCAGGTCACCGTGTTCGTTGTCATTGAGCAGGGCGTCCTGTTCAACACGACGCGGCGGGTTTTGCTTTACGTGCCTGGGGACCCTTTT‑TCGCCCTGGCGCGCGTTCGAGAGCGTCGACAAACTGAACCGCGAACTGGGCCGTTGCTTGCGAGACAAGGCCTATCAGCGTTTCTTCAGCCGCTTCGTGCTGCGGC  >  NC_002947/2738285‑2738570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑