Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,755,392 T→G 8.7% L573R (CTG→CGG)  czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,755,3920TG 8.7% 139.3 / 3.8 46L573R (CTG→CGG) czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (24/18);  new base G (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGCTATGCCCCGGTTCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGATTGCCTCCGACCC  >  NC_002947/2755247‑2755533
                                                                                                                                                 |                                                                                                                                             
cgcTATGCCCCGGTTCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCa                                                                                                                                            <  6:209631/149‑1 (MQ=255)
    aTGCCCCGGTTCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGcc                                                                                                                                        >  4:202427/1‑149 (MQ=255)
             ttCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCGGCACGcgcag                                                                                                                                     <  8:230189/143‑5 (MQ=255)
             ttCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCGGCACGcgcag                                                                                                                                     >  7:230189/1‑139 (MQ=255)
                  gCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCggg                                                                                                                          >  2:232951/1‑149 (MQ=255)
                         tGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGc                                                                                                                   <  1:232951/149‑1 (MQ=255)
                           cTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCt                                                                                                                 <  3:216164/149‑1 (MQ=255)
                                         aCTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTgg                                                                                                   >  4:37136/1‑149 (MQ=255)
                                                 gtgCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCaa                                                                                           >  8:288252/1‑149 (MQ=255)
                                                            gccgccCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTg                                                                                  >  1:254923/1‑147 (MQ=255)
                                                             ccgccCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCAc                                                                                                                       >  3:273813/1‑109 (MQ=255)
                                                             ccgccCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCAc                                                                                                                       <  4:273813/109‑1 (MQ=255)
                                                                        cTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGatcat                                                                    <  3:37136/149‑1 (MQ=255)
                                                                         tgttgtCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGatcatc                                                                   >  3:280895/1‑149 (MQ=255)
                                                                           ttgtCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTgcg                                                                                                                                   >  4:260190/1‑83 (MQ=255)
                                                                           ttgtCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGatcatc                                                                   <  2:254923/147‑1 (MQ=255)
                                                                                gggCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAgg                                                            >  2:119938/1‑149 (MQ=255)
                                                                                  gCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAg                                                             >  6:357579/1‑146 (MQ=255)
                                                                                  gCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAg                                                             <  5:357579/146‑1 (MQ=255)
                                                                                         ggCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGc                                                                                                            >  6:132277/1‑92 (MQ=255)
                                                                                            cAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTg                                                 >  4:197885/1‑148 (MQ=255)
                                                                                            cAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTg                                                 >  7:247863/1‑148 (MQ=255)
                                                                                             aGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAg                                                             <  2:160675/135‑1 (MQ=255)
                                                                                             aGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAg                                                             >  1:160675/1‑135 (MQ=255)
                                                                                                    tGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGgtg                                        <  4:280895/149‑1 (MQ=255)
                                                                                                      ggCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGgtgt                                       <  7:288252/148‑1 (MQ=255)
                                                                                                       gCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGttt                                     >  2:278108/1‑149 (MQ=255)
                                                                                                                  aTCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCgg                          >  5:309703/1‑149 (MQ=255)
                                                                                                                      ccAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAgc                                                                                       <  7:22674/84‑1 (MQ=255)
                                                                                                                      ccAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAgc                                                                                       >  8:22674/1‑84 (MQ=255)
                                                                                                                      ccAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGt                                                  >  7:116883/1‑121 (MQ=255)
                                                                                                                      ccAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGt                                                  <  8:116883/121‑1 (MQ=255)
                                                                                                                           cTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGCCACTGcc                   >  8:211012/1‑147 (MQ=255)
                                                                                                                            tCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCg                                                       <  4:401/110‑1 (MQ=255)
                                                                                                                            tCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCg                                                       >  3:401/1‑110 (MQ=255)
                                                                                                                             cAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCgaga               <  3:197885/149‑1 (MQ=255)
                                                                                                                               gCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGc                                   >  4:3546/1‑127 (MQ=255)
                                                                                                                               gCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGc                                   <  3:3546/127‑1 (MQ=255)
                                                                                                                                  aGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGATTGcc          >  5:219372/1‑149 (MQ=255)
                                                                                                                                         tggtgCCCGGCACGCGCAGGGCCTGCAGGGCGCCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGccc                                 >  8:45397/5‑119 (MQ=37)
                                                                                                                                         tggtgCCCGGCACGCGCAGGGCCTGCAGGGCGCCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGccc                                 <  7:45397/115‑1 (MQ=37)
                                                                                                                                            cGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGATTGCCTCCGAccc  <  6:309703/147‑1 (MQ=255)
                                                                                                                                              cccTGCAGGCCCTGCGCGTGCCGGGTACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTTCCGAGATTg            <  5:44184/135‑1 (MQ=255)
                                                                                                                                              cccTGCAGGCCCTGCGCGTGCCGGGTACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGAttc            >  6:44184/1‑134 (MQ=255)
                                                                                                                                               ccTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGAtt             >  6:201964/1‑133 (MQ=255)
                                                                                                                                               ccTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGAtt             <  5:201964/133‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CGCTATGCCCCGGTTCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGATTGCCTCCGACCC  >  NC_002947/2755247‑2755533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑