Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,755,396 G→C 8.9% Q574H (CAG→CAC)  czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,755,3960GC 8.9% 134.7 / 4.4 45Q574H (CAG→CAC) czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (24/17);  new base C (2/2);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

ATGCCCCGGTTCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGATTGCCTCCGACCCGATGCCGCCGA  >  NC_002947/2755251‑2755544
                                                                                                                                                 |                                                                                                                                                    
aTGCCCCGGTTCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGcc                                                                                                                                                   >  4:202427/1‑149 (MQ=255)
         ttCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCGGCACGcgcag                                                                                                                                                <  8:230189/143‑5 (MQ=255)
         ttCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCGGCACGcgcag                                                                                                                                                >  7:230189/1‑139 (MQ=255)
              gCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCggg                                                                                                                                     >  2:232951/1‑149 (MQ=255)
                     tGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGc                                                                                                                              <  1:232951/149‑1 (MQ=255)
                       cTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCt                                                                                                                            <  3:216164/149‑1 (MQ=255)
                                     aCTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTgg                                                                                                              >  4:37136/1‑149 (MQ=255)
                                             gtgCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCaa                                                                                                      >  8:288252/1‑149 (MQ=255)
                                                        gccgccCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTg                                                                                             >  1:254923/1‑147 (MQ=255)
                                                         ccgccCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCAc                                                                                                                                  >  3:273813/1‑109 (MQ=255)
                                                         ccgccCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCAc                                                                                                                                  <  4:273813/109‑1 (MQ=255)
                                                                    cTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGatcat                                                                               <  3:37136/149‑1 (MQ=255)
                                                                     tgttgtCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGatcatc                                                                              >  3:280895/1‑149 (MQ=255)
                                                                       ttgtCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTgcg                                                                                                                                              >  4:260190/1‑83 (MQ=255)
                                                                       ttgtCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGatcatc                                                                              <  2:254923/147‑1 (MQ=255)
                                                                            gggCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAgg                                                                       >  2:119938/1‑149 (MQ=255)
                                                                              gCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAg                                                                        <  5:357579/146‑1 (MQ=255)
                                                                              gCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAg                                                                        >  6:357579/1‑146 (MQ=255)
                                                                                     ggCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGc                                                                                                                       >  6:132277/1‑92 (MQ=255)
                                                                                        cAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTg                                                            >  4:197885/1‑148 (MQ=255)
                                                                                        cAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTg                                                            >  7:247863/1‑148 (MQ=255)
                                                                                         aGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAg                                                                        <  2:160675/135‑1 (MQ=255)
                                                                                         aGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAg                                                                        >  1:160675/1‑135 (MQ=255)
                                                                                                tGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGgtg                                                   <  4:280895/149‑1 (MQ=255)
                                                                                                  ggCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGgtgt                                                  <  7:288252/148‑1 (MQ=255)
                                                                                                   gCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGttt                                                >  2:278108/1‑149 (MQ=255)
                                                                                                              aTCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCgg                                     >  5:309703/1‑149 (MQ=255)
                                                                                                                  ccAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAgc                                                                                                  >  8:22674/1‑84 (MQ=255)
                                                                                                                  ccAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAgc                                                                                                  <  7:22674/84‑1 (MQ=255)
                                                                                                                  ccAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGt                                                             <  8:116883/121‑1 (MQ=255)
                                                                                                                  ccAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGt                                                             >  7:116883/1‑121 (MQ=255)
                                                                                                                       cTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGCCACTGcc                              >  8:211012/1‑147 (MQ=255)
                                                                                                                        tCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCg                                                                  <  4:401/110‑1 (MQ=255)
                                                                                                                        tCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCg                                                                  >  3:401/1‑110 (MQ=255)
                                                                                                                         cAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCgaga                          <  3:197885/149‑1 (MQ=255)
                                                                                                                           gCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGc                                              >  4:3546/1‑127 (MQ=255)
                                                                                                                           gCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGc                                              <  3:3546/127‑1 (MQ=255)
                                                                                                                              aGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGATTGcc                     >  5:219372/1‑149 (MQ=255)
                                                                                                                                     tggtgCCCGGCACGCGCAGGGCCTGCAGGGCGCCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGccc                                            >  8:45397/5‑119 (MQ=37)
                                                                                                                                     tggtgCCCGGCACGCGCAGGGCCTGCAGGGCGCCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGccc                                            <  7:45397/115‑1 (MQ=37)
                                                                                                                                        cGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGATTGCCTCCGAccc             <  6:309703/147‑1 (MQ=255)
                                                                                                                                          cccTGCAGGCCCTGCGCGTGCCGGGTACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTTCCGAGATTg                       <  5:44184/135‑1 (MQ=255)
                                                                                                                                          cccTGCAGGCCCTGCGCGTGCCGGGTACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGAttc                       >  6:44184/1‑134 (MQ=255)
                                                                                                                                           ccTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGAtt                        >  6:201964/1‑133 (MQ=255)
                                                                                                                                           ccTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGAtt                        <  5:201964/133‑1 (MQ=255)
                                                                                                                                                 ggCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGATTGCCTCCGACCCGATGCCGCCGa  <  6:219372/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATGCCCCGGTTCTGGCCTGGGTGCTGGGCCGGCGCAAACTGGCATGTGCCGCCGCCGCCGCCCTGGTGCTGTTGTCGGGCGTGATGGCCAGCCGCATGGGCAGCGAGTTCATCCCCAGCCTCAGCGAGGGTGACTTCGCCCTGCAGGCCCTGCGCGTGCCGGGCACCAGCCTGTCGCAGTCGGTGGACATGCAACAGCGCCTGGAGCAGGCGATCATCGCCCAGGTGCCGGAGGTGGAGCGGGTGTTTGCCCGCACCGGCACTGCCGAGATTGCCTCCGACCCGATGCCGCCGA  >  NC_002947/2755251‑2755544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑