Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,758,053	T→C	6.3%	S47G (AGC→GGC)	PP_2411 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,758,053	0	T	C	6.3%	183.4 / 2.8	63	S47G (AGC→GGC)	PP_2411	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (33/26);  new base C (2/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

CATCAGCAAGATGGTCAGCGACAGGATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCGGGGGATACCTCATGATGTGCGGACATGCGGGTG  >  NC_002947/2757907‑2758198                                                                                                                                                   |                                                                                                                                                  cATCAGCAAGATGGTCAGCGACAGGATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTgg                                                                                                                                                 >  6:311464/1‑149 (MQ=255) cATCAGCAAGATGGTCAGCGACAGGATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTgg                                                                                                                                                 >  2:256426/1‑149 (MQ=255)  aTCAGCAAGATGGTCAGCGACAGGATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGc                                                                                                                                                <  1:247073/149‑1 (MQ=255)  aTCAGCAAGATGGTCAGCGACAGGATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGc                                                                                                                                                <  8:251288/149‑1 (MQ=255)      gCAAGATGGTCAGCGACAGGATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCg                                                                                                                                               >  7:131984/1‑146 (MQ=255)      gCAAGATGGTCAGCGACAGGATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCg                                                                                                                                               >  2:3455/1‑146 (MQ=255)        aaGATGGTCAGCGACAGGATGCGCTTGCGTCCCAGGCGGTCACCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCgaagaa                                                                                                                                          <  1:84042/149‑1 (MQ=255)          gATGGTCAGCGACAGGATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAAct                                                                                                                                        >  5:133834/1‑149 (MQ=255)                  gCGACAGGATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACtggctggc                                                                                                                                 >  6:299116/1‑148 (MQ=255)                        ggATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGc                                                                                                                          <  1:256426/149‑1 (MQ=255)                              gcTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACATCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGc                                                                                                                     >  5:179347/1‑148 (MQ=255)                               cTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCga                                                                                                                   <  5:299116/149‑1 (MQ=255)                                ttGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGc                                                                                                                     >  3:40118/1‑146 (MQ=255)                                  gCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCgaga                                                                                                                 >  3:314992/1‑148 (MQ=255)                                    gTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACATCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAg                                                                                                              <  6:179347/149‑1 (MQ=255)                                    gTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAg                                                                                                              >  5:350759/1‑149 (MQ=255)                                      cccAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTgg                                                                                                                      >  7:129231/1‑139 (MQ=255)                                      cccAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTgg                                                                                                                      <  8:129231/139‑1 (MQ=255)                                      cccAGGCGGTAGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGcc                                                                                                            >  6:300483/1‑149 (MQ=255)                                         aGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTa                                                                                                         <  6:133834/149‑1 (MQ=255)                                                  cccAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTgg                                                                                                                      >  1:99116/1‑127 (MQ=255)                                                  cccAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTgg                                                                                                                      <  2:99116/127‑1 (MQ=255)                                                   ccAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAg                                                                                               >  4:279573/1‑149 (MQ=255)                                                      aGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTc                                                                                             >  6:333042/1‑148 (MQ=255)                                                        gcgcCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGaa                                                                                          <  1:3455/149‑1 (MQ=255)                                                        gcgcCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGaa                                                                                          <  5:311464/149‑1 (MQ=255)                                                         cgcCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAAc                                                                                         <  7:7901/149‑1 (MQ=255)                                                           ccGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCa                                                                                       >  1:75020/1‑149 (MQ=255)                                                             gAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCAtt                                                                                     >  4:79394/1‑149 (MQ=255)                                                             gAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCAtt                                                                                     >  6:110334/1‑149 (MQ=255)                                                                   gATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTcgacg                                                                                >  6:253816/1‑148 (MQ=255)                                                                      gccgccCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGGAGTCGAACCATTCGACGAAAt                                                                            >  4:267390/1‑149 (MQ=255)                                                                         gccCAAAGTGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCTACGAAATTGc                                                                         <  5:300483/149‑1 (MQ=255)                                                                          cccAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGcc                                                                        >  7:225489/1‑149 (MQ=255)                                                                               aGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAATAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATgg                                                                   <  5:253816/149‑1 (MQ=255)                                                                                          caAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGc                                                         >  6:95252/1‑148 (MQ=255)                                                                                                ccACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATg                                                                    >  4:283383/1‑131 (MQ=255)                                                                                                ccACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATg                                                                    <  3:283383/131‑1 (MQ=255)                                                                                                 cACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGaa                                                                                          <  7:143223/108‑1 (MQ=255)                                                                                                 cACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGaa                                                                                          >  8:143223/1‑108 (MQ=255)                                                                                                 cACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTAccc                                                 >  6:306646/1‑149 (MQ=255)                                                                                                  aCGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCt                                                <  8:225489/149‑1 (MQ=255)                                                                                                            ccGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTg                                      <  2:75020/149‑1 (MQ=255)                                                                                                            ccGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTg                                      >  3:246787/1‑149 (MQ=255)                                                                                                            ccGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTg                                      <  3:79394/149‑1 (MQ=255)                                                                                                            ccGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTg                                      <  4:40118/149‑1 (MQ=255)                                                                                                             cgcgAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGc                                                                                                             >  5:326105/1‑77 (MQ=255)                                                                                                             cgcgAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGc                                                                                                             <  6:326105/77‑1 (MQ=255)                                                                                                                     ttttGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGc                                    >  2:304359/1‑142 (MQ=255)                                                                                                                     ttttGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGc                                    <  1:304359/142‑1 (MQ=255)                                                                                                                          aGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCGGGGGATACCt                         >  2:11024/1‑148 (MQ=255)                                                                                                                            cAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCGGGGGATACCTCat                      >  7:192125/1‑149 (MQ=255)                                                                                                                                 ccACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCGGGGGATACCTCATGAtgt                 <  5:333042/149‑1 (MQ=255)                                                                                                                                     gCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTg                                      <  2:298838/124‑1 (MQ=255)                                                                                                                                     gCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTg                                      >  1:298838/1‑124 (MQ=255)                                                                                                                                        cagttCTTCGCCAGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGAAGGCCGAAGCGGCAATTACCCTGCGCAgg                                         <  6:313798/114‑1 (MQ=255)                                                                                                                                        cagttCTTCGCCAGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGAAGGCCGAAGCGGCAATTACCCTGCGCAgg                                         >  5:313798/5‑118 (MQ=255)                                                                                                                                         gCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCGGGGGATACCTCATGATGTGCGGACAt         >  6:289136/1‑149 (MQ=255)                                                                                                                                          gttCTTCGCCAGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCgggg                                >  1:346234/3‑125 (MQ=255)                                                                                                                                          gttCTTCGCCAGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCgggg                                <  2:346234/123‑1 (MQ=255)                                                                                                                                                cGCTGGCGAAGAACTGGCTGGCTATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCGGGGGATACCTCATGATGTGCGGACATGCGGGt   <  8:131984/148‑1 (MQ=255)                                                                                                                                                cGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCGGGGGATACCTCATGATGTGCGGACATGCgg     <  5:110334/146‑1 (MQ=255)                                                                                                                                                 gCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCGGGGGATACCTCATGATGTGCGGACATGCGGGTg  >  2:219444/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CATCAGCAAGATGGTCAGCGACAGGATGCGCTTGCGTCCCAGGCGGTCGCCCAAGGCGCCGAAGACGATGCCGCCCAAAGGGCGCAGCGCAAAGGCCACGGCGAACACCGCGAAGGTTTTGAGCAAGGCCACGCTGGCGTCTTCGCTGGCGAAGAACTGGCTGGCGATCAGCGTGGCGAGAAAGCCGTACACGGCGAAGTCGAACCATTCGACGAAATTGCCGATGGCCGAAGCGGCAATTACCCTGCGCAGGGTGGCGGGGGATACCTCATGATGTGCGGACATGCGGGTG  >  NC_002947/2757907‑2758198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑