Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,788,078 T→A 12.1% V134D (GTC→GAC)  ahpF → alkyl hydroperoxide reductase subunit F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,788,0780TA12.1% 140.6 / 11.2 58V134D (GTC→GAC) ahpFalkyl hydroperoxide reductase subunit F
Reads supporting (aligned to +/- strand):  ref base T (26/24);  new base A (5/2);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.70e-01

TGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGTTTGGTCA  >  NC_002947/2787946‑2788222
                                                                                                                                    |                                                                                                                                                
tGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAAc                                                                                                                                  <  5:215394/149‑1 (MQ=255)
      cACTGCTGCCAGTGGGGGGCCACCCGTCCCAGGCCCGGGCCGAAGGGATCGAGCAGAGCCAGGCCCTGGAAGGTGAATTCAACTTCGACACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCCTCCAGGCACTGAACCTGATg                                                                                                                            <  8:226193/149‑1 (MQ=255)
            tgcAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAAcc                                                                                                                                 <  7:207320/138‑1 (MQ=255)
            tgcAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAAcc                                                                                                                                 >  8:207320/1‑138 (MQ=255)
              cAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTa                                                                                                                      <  5:33130/147‑1 (MQ=255)
              cAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACt                                                                                                                    >  4:291297/1‑149 (MQ=255)
                  tgggtggCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCGAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAAc                                                                                                                >  4:140798/1‑149 (MQ=255)
                    ggtggCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCaa                                                                                                                 >  4:58258/1‑146 (MQ=255)
                    ggtggCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCaa                                                                                                                 <  3:58258/146‑1 (MQ=255)
                         ccACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCAGTCGTGCCAGAACTGCCCGGCCGTCGGCCAGGCACTGACCCTGATCGCGGAACTCAACCCCAACg                                                                                                         >  8:116666/1‑149 (MQ=255)
                          cACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAg                                                                                                                                           <  1:48997/114‑1 (MQ=255)
                          cACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAg                                                                                                                                           >  2:48997/1‑114 (MQ=255)
                          cACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCCACCCCAACGt                                                                                                        >  7:58334/1‑149 (MQ=255)
                            cccGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTGGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTgc                                                                                                      >  6:357920/1‑149 (MQ=255)
                                  aaaGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGt                                                                                                        >  4:258949/1‑141 (MQ=255)
                                  aaaGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGt                                                                                                        <  3:258949/141‑1 (MQ=255)
                                    aGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGtt                                                                                              >  6:363551/1‑149 (MQ=255)
                                        cAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTa                                                                                                                      >  2:211215/1‑121 (MQ=255)
                                        cAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTa                                                                                                                      <  1:211215/121‑1 (MQ=255)
                                         aGTGCCGAAGTGATCGAGCGGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCAt                                                                                         >  7:211254/1‑149 (MQ=255)
                                            gCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGa                                                                                      <  8:58334/149‑1 (MQ=255)
                                                     aTCGAGCAGATCCAGGCCCCGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAAc                                                                                                                                  <  4:239048/96‑1 (MQ=255)
                                                     aTCGAGCAGATCCAGGCCCCGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAAc                                                                                                                                  >  3:239048/1‑96 (MQ=255)
                                                         aGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGc                                                                                            >  5:331950/1‑130 (MQ=255)
                                                         aGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGc                                                                                            <  6:331950/130‑1 (MQ=255)
                                                          gcggATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTcc                                                                        <  8:211254/146‑1 (MQ=255)
                                                             gATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAg                                                                      >  3:264524/1‑148 (MQ=255)
                                                              aTCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGa                                                                    <  3:291297/149‑1 (MQ=255)
                                                                 cAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACg                                                                  >  3:223933/1‑148 (MQ=255)
                                                                 cAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACg                                                                  >  1:349045/1‑148 (MQ=255)
                                                                   ggCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAg                                                               <  5:363551/149‑1 (MQ=255)
                                                                      ccTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAAc                                                                                                          <  6:36162/103‑1 (MQ=255)
                                                                      ccTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAAc                                                                                                          >  5:36162/1‑103 (MQ=255)
                                                                      ccTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTg                                                             >  6:263563/1‑148 (MQ=255)
                                                                          gAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATc                                                        <  4:264524/149‑1 (MQ=255)
                                                                              gTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATcgcg                                                     >  5:323623/1‑148 (MQ=255)
                                                                               tGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCgcgc                                                    >  8:182739/1‑148 (MQ=255)
                                                                                gAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGTATCgcgc                                                    >  7:259518/1‑147 (MQ=255)
                                                                                   ttCAACTTCGAAACCTACTTCTGGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGAt                                               <  5:357920/149‑1 (MQ=255)
                                                                                     cAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCa                                             >  3:16933/1‑149 (MQ=255)
                                                                                             aaaCCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTa                                     >  1:216053/1‑149 (MQ=255)
                                                                                                 cTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAg                                                                      >  2:261040/1‑112 (MQ=255)
                                                                                                 cTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGa                                 <  4:223933/149‑1 (MQ=255)
                                                                                                 cTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCCGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAg                                                                      <  1:261040/112‑1 (MQ=255)
                                                                                                          gCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTAc                         <  7:182739/148‑1 (MQ=255)
                                                                                                              tcctGCCCGAACTGCCCGGACGGCGTCCAGGCCCTGAACCTGATGGCGGTACTCTACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCaa                    <  8:259518/146‑1 (MQ=255)
                                                                                                                     ttcagtGCCTGGACGACGTCCGGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTAc                         <  8:320962/132‑1 (MQ=255)
                                                                                                                     ttcagtGCCTGGACGACGTCCGGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTAc                         >  7:320962/6‑137 (MQ=255)
                                                                                                                     ttcagtGCCTGGACGACGTCCGGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAg             >  1:60993/6‑149 (MQ=255)
                                                                                                                         gtGCCTGGACGACGTCCGGGCACTGCACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGgtgt         >  8:36724/2‑149 (MQ=255)
                                                                                                                          tGCCTGGACGACGTCCGGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGtt        >  1:135735/1‑149 (MQ=255)
                                                                                                                            cctggACGACGTCCGGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGa              <  5:285375/138‑1 (MQ=255)
                                                                                                                            cctggACGACGTCCGGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGa              >  6:285375/4‑141 (MQ=255)
                                                                                                                            cccGGTCGTCGTCCAGGCACTGAAGCTGATGGCGGTACTGATCCCCAACGTGGGCCATGTTGCCATCGAGGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGTTTg      <  7:116666/149‑1 (MQ=255)
                                                                                                                            cccGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGttt       <  2:216053/148‑1 (MQ=255)
                                                                                                                              cGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGTTTGGt    >  1:103031/1‑149 (MQ=255)
                                                                                                                              cGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGTTTGGt    <  6:13727/149‑1 (MQ=255)
                                                                                                                                gACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACGGAGTATCTACCTCAACGGTGAGGTGTTTGGTCa  <  2:103031/149‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                                
TGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGTTTGGTCA  >  NC_002947/2787946‑2788222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑