Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,799,186 C→T 11.3% intergenic (‑200/+16) PP_2452 ← / ← ansB hypothetical protein/glutaminase‑asparaginase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,799,1860CT11.3% 114.6 / 5.4 45intergenic (‑200/+16)PP_2452/ansBhypothetical protein/glutaminase‑asparaginase
Reads supporting (aligned to +/- strand):  ref base C (20/19);  new base T (3/2);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

GCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCC  >  NC_002947/2799043‑2799328
                                                                                                                                               |                                                                                                                                              
gCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAg                                                                                                                                           <  8:190871/149‑1 (MQ=255)
 cAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgg                                                                                                                                          <  4:167321/149‑1 (MQ=255)
      gCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAAAGAGGCCCTCAGAGCCGGCGCACCGGCACTGAggggg                                                                                                                                       >  2:240133/1‑147 (MQ=255)
      gCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCAGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAggggg                                                                                                                                       >  8:189253/1‑147 (MQ=255)
             aGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACt                                                                                                                                >  6:146368/1‑147 (MQ=255)
             aGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACt                                                                                                                                >  2:9325/1‑147 (MQ=255)
             aGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGCCGCACCGGCCCTGAGGGGGAAGCACt                                                                                                                                <  1:9325/147‑1 (MQ=255)
               gagaAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAg                                                                                                                             >  3:65111/1‑148 (MQ=255)
                     tCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgg                                                                                                                                          <  2:88665/129‑1 (MQ=255)
                       cAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTcc                                                                                                                       >  1:305417/1‑146 (MQ=255)
                                ccTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCTTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGc                                                                                                           <  1:59020/149‑1 (MQ=255)
                                ccTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgg                                                                                                                                          >  3:135562/1‑118 (MQ=255)
                                ccTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgg                                                                                                                                          <  4:135562/118‑1 (MQ=255)
                                ccTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCagaag                                                                                                                 <  2:45274/143‑1 (MQ=255)
                                ccTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCagaag                                                                                                                 >  1:45274/1‑143 (MQ=255)
                                        ccATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCa                                                                                                                              >  3:154869/1‑122 (MQ=255)
                                        ccATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCa                                                                                                                              <  4:154869/122‑1 (MQ=255)
                                           tGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCa                                                                                                                              >  8:16293/1‑119 (MQ=255)
                                           tGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCa                                                                                                                              <  7:16293/119‑1 (MQ=255)
                                                      aGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                                      <  8:93112/148‑1 (MQ=255)
                                                       gAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGt                                                                                    >  5:6182/1‑149 (MQ=255)
                                                        aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCagaa                                                                                                                  >  6:55558/1‑118 (MQ=255)
                                                        aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCagaa                                                                                                                  <  5:55558/118‑1 (MQ=255)
                                                          aGGTAAACGAGGTCGCCGGTTAGTAACCTCTGTGTCTACGCGTTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCGGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCtt                                                                                 <  8:333829/149‑1 (MQ=255)
                                                           ggTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAg                                                                                                                                           <  6:93551/90‑1 (MQ=38)
                                                           ggTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAg                                                                                                                                           >  5:93551/1‑90 (MQ=38)
                                                           ggTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTg                                                                                <  5:146368/149‑1 (MQ=255)
                                                                 cGATGTCTACGGTTTGTAAGCTCTGTGTCTACGCTGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCATGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATc                                                                          <  4:65111/149‑1 (MQ=255)
                                                                    tGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCtt                                                                                                 <  3:93064/123‑1 (MQ=255)
                                                                    tGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCtt                                                                                                 >  4:93064/1‑123 (MQ=255)
                                                                               tGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTATCCTGGGTCTTGGTCATCGCTACCATCGCCa                                                             <  2:305417/148‑1 (MQ=255)
                                                                                    ccTCTGTGTCTACGCGGTTGATGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATcc                                                       <  3:284421/149‑1 (MQ=255)
                                                                                     ctctGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCcg                                                      >  7:153367/1‑149 (MQ=255)
                                                                                              tACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTg                                             >  6:92007/1‑149 (MQ=255)
                                                                                                cgcgGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGc                                            <  6:6182/148‑1 (MQ=255)
                                                                                                    gTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTAGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTAGCGGGt                                        >  2:300894/1‑148 (MQ=255)
                                                                                                     ttGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGtt                                       >  1:137780/1‑148 (MQ=255)
                                                                                                        aGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAg                                    <  5:92007/148‑1 (MQ=255)
                                                                                                               ggCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                             <  8:153367/148‑1 (MQ=255)
                                                                                                                gCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGc                           >  4:65902/1‑149 (MQ=255)
                                                                                                                          ccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAg                 >  2:108523/1‑149 (MQ=255)
                                                                                                                           cTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGt                >  8:333007/1‑149 (MQ=255)
                                                                                                                              agagCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGt             >  5:89239/1‑149 (MQ=255)
                                                                                                                                       gcACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCAt    >  7:328083/1‑149 (MQ=255)
                                                                                                                                         aCCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATcc  >  8:33416/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                              
GCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCC  >  NC_002947/2799043‑2799328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑