Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,812,778	C→G	9.2%	intergenic (+72/‑38)	rplT → / → pheS	50S ribosomal protein L20/phenylalanine‑‑tRNA ligase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,812,778	0	C	G	9.2%	199.1 / 10.2	65	intergenic (+72/‑38)	rplT/pheS	50S ribosomal protein L20/phenylalanine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (30/29);  new base G (3/3);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

GCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGC  >  NC_002947/2812635‑2812923                                                                                                                                                |                                                                                                                                                   gCTGATCTGGCAGTGAATGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGc                                                                                                                                               >  6:206639/1‑149 (MQ=255)        tGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTccc                                                                                                                                       >  6:338284/1‑149 (MQ=255)        tGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTccc                                                                                                                                       >  3:304859/1‑149 (MQ=255)          gCAGTGAATGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGC‑TCTTcc                                                                                                                                        <  5:206639/146‑1 (MQ=255)          gCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTc                                                                                                                                         <  3:99973/145‑1 (MQ=255)          gCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTc                                                                                                                                         <  8:20694/145‑1 (MQ=255)                aaCGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGc                                                                                                                                               <  7:110398/134‑1 (MQ=255)                aaCGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGc                                                                                                                                               >  8:110398/1‑134 (MQ=255)                aaCGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTc                                                                                                                               <  5:338284/149‑1 (MQ=255)                      aaaGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCt                                                                                                                         <  3:315425/149‑1 (MQ=255)                       aaGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTg                                                                                                                        <  3:239517/149‑1 (MQ=255)                               tttGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTc                                                                                                                               >  3:93183/1‑134 (MQ=255)                               tttGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTc                                                                                                                               <  4:93183/134‑1 (MQ=255)                               tttGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGt                                                                                                                <  4:304859/149‑1 (MQ=255)                               tttGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGt                                                                                                                <  3:340708/149‑1 (MQ=255)                                    tgcGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATg                                                                                                           >  4:96234/1‑149 (MQ=255)                                     gcGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTg                                                                                                                        <  2:123318/135‑1 (MQ=255)                                     gcGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTg                                                                                                                        >  1:123318/1‑135 (MQ=255)                                      cGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATgg                                                                                                          >  4:73840/1‑148 (MQ=255)                                      agATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATg                                                                                                           <  2:104731/146‑1 (MQ=255)                                         ttGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGaaa                                                                                                       >  2:184261/1‑148 (MQ=255)                                            tCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCt                                                                                                   >  1:28828/1‑149 (MQ=255)                                              gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACa                                                                                                             <  2:131598/137‑1 (MQ=255)                                              gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACa                                                                                                             >  1:131598/1‑137 (MQ=255)                                              gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACAt                                                                                                            <  3:155687/138‑1 (MQ=255)                                              gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACAt                                                                                                            >  4:155687/1‑138 (MQ=255)                                              gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTg                                                                                                  >  3:148610/1‑148 (MQ=255)                                              gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGAACATGGAAAACCTg                                                                                                  >  5:217094/1‑148 (MQ=255)                                                gaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGAt                                                                                               >  8:79634/1‑149 (MQ=255)                                                gaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGAt                                                                                               <  2:28828/149‑1 (MQ=255)                                                      tAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTg                                                                                         >  2:303716/1‑149 (MQ=255)                                                       aaaGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTg                                                                                                                        <  8:69307/117‑1 (MQ=255)                                                       aaaGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTg                                                                                                                        >  7:69307/1‑117 (MQ=255)                                                         aGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGtc                                                                                      <  3:96234/149‑1 (MQ=255)                                                           ccGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGAACATGGAAAACCTGGATGCGCTGGtctc                                                                                    <  6:217094/149‑1 (MQ=255)                                                                  ggCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGcc                                                                             >  2:124024/1‑149 (MQ=255)                                                                     ttAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTa                                                                          >  6:7621/1‑149 (MQ=255)                                                                       aaGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTa                                                                          <  5:7621/147‑1 (MQ=255)                                                                             ccGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGccc                                                                            >  8:200292/1‑139 (MQ=255)                                                                             ccGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGccc                                                                            <  7:200292/139‑1 (MQ=255)                                                                              cgcgACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTg                                                                 >  2:183136/1‑149 (MQ=255)                                                                               gcgACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTgg                                                                <  3:73840/149‑1 (MQ=255)                                                                                     aTCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCAGGTTCCCCTATTTTCGTATCTGGAGTATGTACATGGAAAACCTGGATGCGCTGGTCTCCAAAGCCCTAGAGGCCGTGGAAcgc                                                           >  1:278833/1‑149 (MQ=255)                                                                                          ccGGCAGCGCTAGCACCGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCt                                                                                                   <  5:111797/103‑1 (MQ=255)                                                                                          ccGGCAGCGCTAGCACCGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCt                                                                                                   >  6:111797/1‑103 (MQ=255)                                                                                             gcggTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATgcgc                                                                                           >  6:168335/1‑108 (MQ=255)                                                                                             gcggTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGAc                                                  <  4:345894/149‑1 (MQ=255)                                                                                               ggTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACAt                                                <  1:303716/149‑1 (MQ=255)                                                                                                  gCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTagag                                                                       <  4:38584/123‑1 (MQ=255)                                                                                                  gCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTagag                                                                       >  3:38584/1‑123 (MQ=255)                                                                                                            ccGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGaa                                   >  7:42469/1‑149 (MQ=255)                                                                                                              gggCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGaa                                   >  1:43890/1‑147 (MQ=255)                                                                                                                       aCGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGt                        <  2:278833/149‑1 (MQ=255)                                                                                                                            aTCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAAtt                   <  1:124024/149‑1 (MQ=255)                                                                                                                                 tAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTAtctc              <  1:183136/149‑1 (MQ=255)                                                                                                                                   ggggAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGccc                                        <  6:66125/121‑1 (MQ=255)                                                                                                                                     ggAAGAGCTTTGAAGGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGAt                                                                                               >  5:102554/1‑64 (MQ=25)                                                                                                                                     ggAAGAGCTTTGAAGGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGAt                                                                                               <  6:102554/64‑1 (MQ=25)                                                                                                                                     ggAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCgg                          <  8:184283/133‑1 (MQ=255)                                                                                                                                     ggAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGc           >  6:69196/1‑148 (MQ=255)                                                                                                                                      gAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGc           >  2:155211/1‑147 (MQ=255)                                                                                                                                         gagCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCaag        >  6:215115/1‑147 (MQ=255)                                                                                                                                              ttCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAgg    <  7:28733/146‑1 (MQ=255)                                                                                                                                              ttCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAgg    >  8:28733/1‑146 (MQ=255)                                                                                                                                              ttCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGc  >  2:244050/1‑148 (MQ=255)                                                                                                                                                |                                                                                                                                                   GCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGC‑TCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGC  >  NC_002947/2812635‑2812923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑