| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,852,397 | T→A | 9.9% | Q312L (CAG→CTG) | PP_2505 ← | GAF domain/GGDEF domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,852,397 | 0 | T | A | 9.9% | 158.9 / 8.5 | 61 | Q312L (CAG→CTG) | PP_2505 | GAF domain/GGDEF domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (28/27); new base A (3/3); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01 | |||||||||||
ATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATTC > NC_002947/2852258‑2852545 | aTGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCAGGGTGAGc < 2:179455/148‑1 (MQ=255) cGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAgg < 1:338039/148‑1 (MQ=255) cccTCCCGGATCCGGAGATCTTAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCa > 3:84163/1‑149 (MQ=255) ccTCCCGGATCCGGAGATCTTAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCAc > 5:310146/1‑149 (MQ=255) cccgATCCGGACATCTTAACTTCCTCA‑TGCTAACGCTCGCACCTAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGCGCCCTGCTCACCCa < 4:206797/145‑1 (MQ=255) ccGGATCCGGAGATCTTAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCaa > 4:266162/1‑149 (MQ=255) gATCCGGAGATCTTAACTTCCTCAGGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATg > 1:3183/1‑149 (MQ=255) gATCCGGAGATCTTAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGc < 2:3183/149‑1 (MQ=255) ggAGATCTTAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATg < 3:160270/149‑1 (MQ=255) ttAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATgg > 1:106178/1‑143 (MQ=255) ttAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATgg < 2:106178/143‑1 (MQ=255) cTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCg < 4:84163/149‑1 (MQ=255) cTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAggg > 6:289936/1‑149 (MQ=255) cA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCa > 4:154075/1‑119 (MQ=255) gATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGCCCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGgccgcc < 3:6411/149‑1 (MQ=255) tAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAgg > 1:88300/1‑141 (MQ=255) tAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAgg < 2:88300/141‑1 (MQ=255) tAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGAACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCgg > 6:302168/1‑149 (MQ=255) aaCGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCggg > 4:96772/1‑149 (MQ=255) gCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTcc < 6:125674/129‑1 (MQ=255) acaTAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACa < 1:311952/145‑1 (MQ=255) acaTAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACa < 1:316470/145‑1 (MQ=255) acaTAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACa > 2:316470/1‑145 (MQ=255) acaTAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACa > 2:311952/1‑145 (MQ=255) acaTAGACAACTTGGTAACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCa < 1:203743/121‑1 (MQ=255) acaTAGACAACTTGGTAACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCa > 2:203743/1‑121 (MQ=255) gACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGgc < 3:266162/148‑1 (MQ=255) aCAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGgcgc > 2:348158/1‑149 (MQ=255) aCTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGTGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCt < 5:289936/149‑1 (MQ=255) tCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAggg > 3:274187/1‑117 (MQ=255) tCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAggg < 4:274187/117‑1 (MQ=255) cACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGAGCACACAATGATCATGGTCATCTACCGAGGGCCGACGGGTAACAGCAACGGCGCGCTGCCGAtt > 4:242859/1‑149 (MQ=255) aCGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCgg > 4:287857/1‑122 (MQ=255) aCGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGAtt < 3:96772/148‑1 (MQ=255) ccGCATAGAGACCGCCGTCAGCGCGCTTTATTACATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTcc > 1:218768/1‑149 (MQ=255) cGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGAtt < 3:195774/130‑1 (MQ=255) cGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGAtt > 4:195774/1‑130 (MQ=255) cGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCg > 3:252958/1‑149 (MQ=255) gCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAAAAGAAACCGCGCGCTTCCGATTGACTGACGCAAGCGCTcct > 8:312684/1‑147 (MQ=255) cgccgTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgcgc > 7:197605/1‑148 (MQ=255) ccgTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGAt > 3:308246/1‑116 (MQ=255) ccgTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGAt < 4:308246/116‑1 (MQ=255) aGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCtt < 1:348158/149‑1 (MQ=255) cgcgcTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTgg < 8:197605/149‑1 (MQ=255) gcgcTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGc < 8:14153/149‑1 (MQ=255) gcTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTa > 4:183972/1‑149 (MQ=255) ttGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCttt > 7:28404/1‑148 (MQ=255) atcaATCTGCTCACCCTTGGTGATCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACTGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGt < 7:312684/148‑1 (MQ=255) tcaATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAg > 1:94214/1‑149 (MQ=255) cccTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGc > 4:110943/3‑147 (MQ=255) cTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgc < 4:254283/115‑1 (MQ=255) cTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgc > 3:254283/1‑115 (MQ=255) cTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTa > 5:339478/1‑143 (MQ=255) cTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTa < 6:339478/143‑1 (MQ=255) gCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATtgactg < 1:61788/91‑1 (MQ=255) gCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATtgactg > 2:61788/1‑91 (MQ=255) gCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgc < 6:159329/113‑1 (MQ=255) aCCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTAt < 2:163135/125‑1 (MQ=255) aCCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTAt > 1:163135/1‑125 (MQ=255) cccTTGGTTATCATGGCGCAACATATGCTGATGGTCATCTTCCGAGCGCCGCCGGGTAACATAAACGCCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGaa < 5:302168/149‑1 (MQ=255) cccTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGaa < 2:94214/149‑1 (MQ=255) tGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATtc > 3:138068/1‑149 (MQ=255) | ATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCA‑GGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATTC > NC_002947/2852258‑2852545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |