Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,869,095 T→A 6.9% intergenic (+634/‑297) PP_2520 → / → PP_2522 hypothetical protein/transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,869,0950TA 6.9% 177.9 / 3.1 58intergenic (+634/‑297)PP_2520/PP_2522hypothetical protein/transposase
Reads supporting (aligned to +/- strand):  ref base T (25/29);  new base A (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

GACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGTGAGCAGATCCTGACGCGGCGCCAGACCCAACAAGTGAA  >  NC_002947/2868949‑2869225
                                                                                                                                                  |                                                                                                                                  
gACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTAAc                                                                                                                                  >  5:152412/1‑149 (MQ=255)
    gCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCa                                                                                                                              <  6:327697/149‑1 (MQ=255)
      tttCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAg                                                                                                                             >  1:101113/1‑148 (MQ=255)
        tCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAg                                                                                                                             <  2:101113/146‑1 (MQ=255)
        tCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGt                                                                                                                            <  2:126592/147‑1 (MQ=255)
          aGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACt                                                                                                                        <  4:20462/149‑1 (MQ=255)
          aGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACt                                                                                                                        >  4:101565/1‑149 (MQ=255)
           ggCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGATACTTCAGTTACTg                                                                                                                       >  3:239457/1‑149 (MQ=255)
            gCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTgc                                                                                                                      >  1:59350/1‑149 (MQ=255)
               aCCCTCACATCTCGATCAATACCCGGGTCGCTGATTGGGAATATCAGTTTCGCGCGCGCATCGCTGCCATGGCGTACCTGATGCAGTCTTTTTGCAACTTCCATAACGAGGGCGAGACCGTGCTGCGCAGTTACTTCAGTTACTGCGCa                                                                                                                   <  1:30472/149‑1 (MQ=255)
               aCCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTAACTGAAGTAACTGCGCa                                                                                                                   <  6:152412/149‑1 (MQ=255)
                 ccTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACt                                                                                                                 >  5:95043/1‑149 (MQ=255)
                 ccTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACt                                                                                                                 <  3:101565/149‑1 (MQ=255)
                   tCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTg                                                                                                                >  8:56959/1‑148 (MQ=255)
                            gATAAAAACCCGGGTAGCTGATTCGGAATATCAGTTTCGCGCGAGCAACGCTGCCATGTCATACCTCATGCACTCATTTGGCAACTTCCATAAAGAGGTCGAGACGGTAAAGCTCAGTTACTTCAGTTACTGCACAATGCAAATGAACt                                                                                                      >  2:264619/1‑149 (MQ=255)
                                aaTACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGcc                                                                                                   <  1:173151/148‑1 (MQ=255)
                                             cTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCgg                                                                                     <  5:142723/149‑1 (MQ=255)
                                              tGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCggg                                                                                    >  2:74308/1‑149 (MQ=255)
                                                aTTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCggg                                                                                    <  1:264619/147‑1 (MQ=255)
                                                aTTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCa                                                                                  >  4:146612/1‑149 (MQ=255)
                                                   cGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCAtt                                                                                <  2:240082/148‑1 (MQ=255)
                                                   cGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTc                                                                               >  8:124543/1‑149 (MQ=255)
                                                    ggAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCt                                                                              >  2:213084/1‑149 (MQ=255)
                                                     gAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTg                                                                             >  5:255031/1‑149 (MQ=255)
                                                        tatCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCtt                                                                          >  5:119802/1‑149 (MQ=255)
                                                          tCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTAc                                                                                                                         <  1:346682/100‑1 (MQ=255)
                                                          tCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTAc                                                                                                                         >  2:346682/1‑100 (MQ=255)
                                                          tCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTcc                                                                        <  1:74308/149‑1 (MQ=255)
                                                          tCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTcc                                                                        >  6:191091/1‑149 (MQ=255)
                                                             gTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTgg                                                                     <  7:56959/149‑1 (MQ=255)
                                                             gTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTgg                                                                     <  7:124543/149‑1 (MQ=255)
                                                                  gcgcgcgcAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCaa                                                                 <  6:255031/148‑1 (MQ=255)
                                                                  gcgcgcgcAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCaa                                                                 <  6:95043/148‑1 (MQ=255)
                                                                       cgcAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATgg                                                           <  6:119802/149‑1 (MQ=255)
                                                                          aaCGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTg                                                                      <  8:67275/135‑1 (MQ=255)
                                                                          aaCGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTg                                                                      >  7:67275/1‑135 (MQ=255)
                                                                           aCGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTc                                                       <  5:191091/149‑1 (MQ=255)
                                                                             gCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATggg                                                          <  1:114742/144‑1 (MQ=255)
                                                                             gCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATggg                                                          >  2:114742/1‑144 (MQ=255)
                                                                                     ggCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCAt                                                                                 >  8:328256/1‑113 (MQ=255)
                                                                                        gTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTACTGGCCAATGATGGGTTCTGCAAGCACAGCg                                          <  1:213084/149‑1 (MQ=255)
                                                                                          aCCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGt                                        >  4:166120/1‑149 (MQ=255)
                                                                                           ccTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGt                                        <  3:146612/148‑1 (MQ=255)
                                                                                               aTGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCAATCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGTGAGc                                    <  1:245951/148‑1 (MQ=255)
                                                                                                   aGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGc                                                    >  5:244892/1‑128 (MQ=255)
                                                                                                   aGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGc                                                    <  6:244892/128‑1 (MQ=255)
                                                                                                     tCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCaa                                                  <  7:198903/128‑1 (MQ=255)
                                                                                                     tCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCaa                                                  >  8:198903/1‑128 (MQ=255)
                                                                                                       aTTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGTGAGCAGATCCTGa                           <  3:166120/149‑1 (MQ=255)
                                                                                                           ggCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGTGAGCAGATCCTGAcgcg                       >  4:45748/1‑149 (MQ=255)
                                                                                                                    aaTAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGTGAGCAGATCCTGACGCGGCGCCAGAc              >  6:147066/2‑149 (MQ=255)
                                                                                                                      tAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGTGAGCAGATCCTGACGCGGCGCCAGAccc            <  2:59350/149‑1 (MQ=255)
                                                                                                                       aaCGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCt                                                      <  4:269261/106‑1 (MQ=255)
                                                                                                                        aCGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGTGa                                      >  4:319635/1‑121 (MQ=255)
                                                                                                                            ggTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGTGAGCAGATCCTGACGCGGCGCCAGACCCAACAAg      >  6:354247/1‑149 (MQ=255)
                                                                                                                                gagaCGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGTGAGCAGATCCTGACGCGGCGCCAGACCCAACAAGTGaa  <  3:45748/149‑1 (MQ=255)
                                                                                                                                        agtgcGCAGTAACTGAAGTAACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGc                                                    >  6:308416/3‑91 (MQ=38)
                                                                                                                                        agtgcGCAGTAACTGAAGTAACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGc                                                    <  5:308416/89‑1 (MQ=38)
                                                                                                                                                  |                                                                                                                                  
GACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGCGCGCAACGCTGCCATGGCGTACCTGATGCAGTCATTTGGCAACTTCCATAACGAGGTCGAGACGGTGCTGCGCAGTTACTTCAGTTACTGCGCACTGCAAATGAACTGCCTGGACCTGGCGCGGGCATTCTGCTTCCTGGCCAATGATGGGTTCTGCAAGCACAGCGGTGAGCAGATCCTGACGCGGCGCCAGACCCAACAAGTGAA  >  NC_002947/2868949‑2869225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑