Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,880,503 G→A 8.0% S115S (TCG→TCA)  PP_2535 → transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,880,5030GA 8.0% 143.0 / 2.1 50S115S (TCG→TCA) PP_2535transport protein
Reads supporting (aligned to +/- strand):  ref base G (24/22);  new base A (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

CGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGAAATGCTGGGTTGGCGCACGGCGTTCTGGG  >  NC_002947/2880363‑2880648
                                                                                                                                            |                                                                                                                                                 
cgcCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTcgc                                                                                                                                           <  1:324677/149‑1 (MQ=255)
   cGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGc                                                                                                                                        >  8:13836/1‑149 (MQ=255)
                   ggCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGccc                                                                                                                        <  4:319345/149‑1 (MQ=255)
                        aTGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCgag                                                                                                                     >  3:328258/1‑147 (MQ=255)
                          ggCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAgc                                                                                                                 >  7:60859/1‑149 (MQ=255)
                          ggCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAgc                                                                                                                 >  6:20842/1‑149 (MQ=255)
                             cATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAgcgcg                                                                                                              >  8:167104/1‑149 (MQ=255)
                               ttttCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCa                                                                                                                                      >  4:226246/1‑123 (MQ=255)
                               ttttCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCa                                                                                                                                      <  3:226246/123‑1 (MQ=255)
                                     ccTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGcc                                                                                                      >  2:119350/1‑149 (MQ=255)
                                           gCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCt                                                                                                <  6:256063/149‑1 (MQ=255)
                                            cAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTg                                                                                               <  4:328258/149‑1 (MQ=255)
                                                cTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAAt                                                                                           >  3:9219/1‑149 (MQ=255)
                                                      tCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCAt                                                                                     <  8:60859/149‑1 (MQ=255)
                                                                    cGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTgg                                                                       <  4:9219/149‑1 (MQ=255)
                                                                    cGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTgg                                                                       <  5:20842/149‑1 (MQ=255)
                                                                     gACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGc                                                                      >  6:213425/1‑149 (MQ=255)
                                                                        tACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGccac                                                                   >  7:151332/1‑149 (MQ=255)
                                                                               gCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATcggc                                                            >  5:170676/1‑149 (MQ=255)
                                                                                    ctgGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTgg                                                                                                  <  8:102115/106‑1 (MQ=255)
                                                                                    ctgGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTgg                                                                                                  >  7:102115/1‑106 (MQ=255)
                                                                                       gtggCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGcc                                                    >  6:101220/1‑149 (MQ=255)
                                                                                         ggCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGTCACCATCGGCGGCGTGCCGt                                                  >  5:125354/1‑149 (MQ=255)
                                                                                          gCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGtt                                                 <  6:170676/149‑1 (MQ=255)
                                                                                           cccGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTg                                                <  5:101220/149‑1 (MQ=255)
                                                                                                gTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGc                                                     >  3:193175/1‑139 (MQ=255)
                                                                                                gTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGc                                                     <  4:193175/139‑1 (MQ=255)
                                                                                                     cacttcactCAACCACGGCGCGTTCTTTGGCATCGGCTCAATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCa                                                                                      <  1:11892/101‑1 (MQ=255)
                                                                                                     cacttcactCAACCACGGCGCCTTCTTTGGCATCGGCTCAATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCa                                                                                      >  2:6214/1‑101 (MQ=255)
                                                                                                     cacttcactCAACCACGGCGCCTTCTTTGGCATCGGCTCAATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCa                                                                                      <  1:6214/101‑1 (MQ=255)
                                                                                                     cacttcactCAACCACGGCGCCTTCTTTGGCATCGGCTCAATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCa                                                                                      >  2:11892/1‑101 (MQ=255)
                                                                                                      acttcactCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGtt                                     >  3:143400/1‑149 (MQ=255)
                                                                                                      acttcactCAACCACGGCGCCTTCTTTGGCATCGGCTCGATAGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATCTTCATGGGTTTGACCCTGCCCTCCAACGGCGGCGTGCCGTTGGCGACCTGGtt                                     >  5:37205/1‑149 (MQ=255)
                                                                                                        ttcactCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCg                                                   >  3:201944/1‑133 (MQ=255)
                                                                                                        ttcactCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCg                                                   <  4:201944/133‑1 (MQ=255)
                                                                                                          cactCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTgg                                       <  1:56039/143‑1 (MQ=255)
                                                                                                          cactCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTgg                                       >  2:56039/1‑143 (MQ=255)
                                                                                                          cactCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTggg                                  <  8:151332/148‑1 (MQ=255)
                                                                                                           actCAACAACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCAAGTGTGCTGCCGCGCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCTTGCCGTTGGCGACCTGGTTGGGTg                                <  6:37205/149‑1 (MQ=255)
                                                                                                             tCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGa                               >  5:268333/1‑148 (MQ=255)
                                                                                                             tCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGa                               >  6:23376/1‑148 (MQ=255)
                                                                                                                   aCGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTg                                        >  4:36980/1‑133 (MQ=255)
                                                                                                                   aCGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTg                                        <  3:36980/133‑1 (MQ=255)
                                                                                                                     ggCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGAAATGCTggg                      <  7:13836/149‑1 (MQ=255)
                                                                                                                         ccTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGAAATGCTGGGTTgg                  <  4:143400/149‑1 (MQ=255)
                                                                                                                          cttcttTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGAAATGCTGGGTTGgc                 <  5:213425/149‑1 (MQ=255)
                                                                                                                          cttcttTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGAAATGCTGGGTTGgc                 >  1:33354/1‑149 (MQ=255)
                                                                                                                                       ggCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGAAATGCTGGGTTGGCGCACGGCGTTCt     <  5:23376/148‑1 (MQ=255)
                                                                                                                                         cTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGAAATGCTGGGTTGGCGCACGGCGTTCTggg  >  2:111697/1‑149 (MQ=255)
                                                                                                                                         cTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTAGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGAAATGCTGGGTTGGCGCACGGCGTTCTggg  <  1:111697/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
CGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACTCAACCACGGCGCCTTCTTTGGCATCGGCTCGATCGTCGCGGCCAGTGTGGTGCCGCCCGAGAAGCGCGCCGGGGCCGTGGCTGCAATGTTCATGGGTTTGACCCTGGCCACCATCGGCGGCGTGCCGTTGGCGACCTGGTTGGGTGAAATGCTGGGTTGGCGCACGGCGTTCTGGG  >  NC_002947/2880363‑2880648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑