Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,887,552 G→A 8.9% L373L (CTG→TTG)  PP_2542 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,887,5520GA 8.9% 124.9 / 2.4 45L373L (CTG→TTG) PP_2542GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (25/16);  new base A (2/2);  total (27/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

GCATCGTCCAGCAACGCGGCACTGTCGGGAATC‑GCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCC  >  NC_002947/2887404‑2887698
                                                                                                                                                      |                                                                                                                                                  
gcATCGTCCAGCAACGCGGCACTGTCGGGAATC‑GCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAg                                                                                                                                                    <  8:226417/149‑1 (MQ=255)
gcATCGTCCAGCAACGCGGCACTGTCGGGAATC‑GCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAg                                                                                                                                                    <  3:34185/149‑1 (MQ=255)
  aTCGTCCAGCAACGCGGCACTGTCGGGAATC‑GCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGgc                                                                                                                                                  >  5:130951/1‑149 (MQ=255)
    cGTCCAGCAACGCGGCACTGTCGGGAATC‑GCCGGATGGCGGGTCACAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGgcg                                                                                                                                                 >  1:72349/1‑149 (MQ=255)
      tCCAGCAACGCGGCACTGTCGGGAATCTGCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAAATTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGgcgat                                                                                                                                               >  1:14443/1‑147 (MQ=255)
        cAGCAACGCGGCACTGTCGGGAATC‑GCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGa                                                                                                                                            <  2:72349/149‑1 (MQ=255)
         aGCAACGCGGCACTGTCGGGAATC‑GCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAg                                                                                                                                           <  5:43984/149‑1 (MQ=255)
         aGCAACGCGGCACTGTCGGGAATC‑GCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAg                                                                                                                                           <  1:276709/149‑1 (MQ=255)
             aCGCGGCACTGTCGGGAATC‑GCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGc                                                                                                                                       <  5:99252/149‑1 (MQ=255)
                     cTGTCGGGAATC‑GCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCa                                                                                                                               <  6:130951/149‑1 (MQ=255)
                                                    gATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATAcacca                                                                                                  >  6:336315/1‑149 (MQ=255)
                                                          cATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                                             <  5:131851/148‑1 (MQ=255)
                                                             gctttCATCCTATTCGTTGAAAAATTAAATGCCCTGTCGATGCAGATGCCGGCAAACTTCCTGATGCGCCGCGGCCAGGCGCTGGCGCTGGTGTTTTAGGTGGTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                                          <  2:14443/145‑1 (MQ=255)
                                                              ccTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCa                                                                                         >  1:122417/1‑148 (MQ=255)
                                                                ttCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgc                                                                                                                             >  3:233647/1‑110 (MQ=255)
                                                                ttCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgc                                                                                                                             <  4:233647/110‑1 (MQ=255)
                                                                 tCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCAc                                                                                     >  7:9417/1‑149 (MQ=255)
                                                                       gATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                                 >  2:144026/1‑147 (MQ=255)
                                                                         ttCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCGAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGAGCTCCATCACCTGCGAgg                                                                             >  6:93497/1‑149 (MQ=255)
                                                                            ggTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAggtg                                                                           <  3:44864/148‑1 (MQ=255)
                                                                                aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATa                                                                                                       >  7:60073/1‑116 (MQ=255)
                                                                                aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                      >  4:111984/1‑149 (MQ=255)
                                                                                aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                      >  5:187161/1‑149 (MQ=255)
                                                                                aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGcctgg                                                                                                                       <  3:259355/100‑4 (MQ=21)
                                                                                aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGcctgg                                                                                                                       >  4:259355/1‑97 (MQ=21)
                                                                                              cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgcgc                                                                                                                           >  2:342965/1‑82 (MQ=255)
                                                                                              cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgcgc                                                                                                                           <  1:342965/82‑1 (MQ=255)
                                                                                               aGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCCTCACCTGCGAGGTGGTCAGGCCTGAGCGCATCtt                                                       >  1:160688/1‑149 (MQ=255)
                                                                                                    aTGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCACCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGt                                                  >  6:268699/1‑149 (MQ=255)
                                                                                                     tGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTg                                                 >  4:328822/1‑149 (MQ=255)
                                                                                                           tGCCGGCCAACTTCCTGATGCGCCTGGGCCAGGGGCTGGCGCAGGCGGTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTgcagca                                            <  8:9417/148‑1 (MQ=255)
                                                                                                                     cTTCCTGATGCGCCTCGGCCCGGCGCGGGCGCCGGGGCTTGAGTGGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATc                                 <  6:187161/149‑1 (MQ=255)
                                                                                                                          tGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                            >  4:83587/1‑149 (MQ=255)
                                                                                                                          tGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                            >  4:78792/1‑149 (MQ=255)
                                                                                                                            aTGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                            <  3:111984/147‑1 (MQ=255)
                                                                                                                            aTGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCg                           <  1:144026/148‑1 (MQ=255)
                                                                                                                              gcgcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                      <  4:105161/103‑1 (MQ=255)
                                                                                                                              gcgcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                      >  3:105161/1‑103 (MQ=255)
                                                                                                                                 ccTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCAACTGCGAGGTGGTCCGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                     >  8:47448/1‑149 (MQ=255)
                                                                                                                                 ccTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCAAGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                     >  8:42082/1‑149 (MQ=255)
                                                                                                                                   tCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCa                                   >  1:143177/1‑133 (MQ=255)
                                                                                                                                      gCCAGGCGCTGGCGCAGGCGCTTGAGGTGCATGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGCTGGTCAGGCCTGAGCGCATCATCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTgg                    >  1:31253/1‑145 (MQ=255)
                                                                                                                                        cAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGGGTACAGCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGttttt                                      >  5:246950/1‑125 (MQ=255)
                                                                                                                                        cAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCa              <  3:328822/149‑1 (MQ=255)
                                                                                                                                           gcgcTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                     >  7:280725/1‑139 (MQ=38)
                                                                                                                                           gcgcTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                     <  8:280725/139‑1 (MQ=38)
                                                                                                                                             gcTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAg         >  3:13595/1‑149 (MQ=255)
                                                                                                                                                    ccggCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGAccc  <  1:183903/147‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GCATCGTCCAGCAACGCGGCACTGTCGGGAATC‑GCCGGATGGCGGGTC‑CAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCC  >  NC_002947/2887404‑2887698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑