Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,887,559 G→C 9.1% L370L (CTC→CTG) ‡ PP_2542 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,887,5590GC 9.1% 116.2 / 4.8 44L370L (CTC→CTG) ‡PP_2542GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (23/16);  new base C (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.95e-01

CAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACG  >  NC_002947/2887412‑2887703
                                                                                                                                                   |                                                                                                                                                
cAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGa                                                                                                                                                 <  2:72349/149‑1 (MQ=255)
 aGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAg                                                                                                                                                <  5:43984/149‑1 (MQ=255)
 aGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAg                                                                                                                                                <  1:276709/149‑1 (MQ=255)
     aCGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGc                                                                                                                                            <  5:99252/149‑1 (MQ=255)
             cTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCa                                                                                                                                    <  6:130951/149‑1 (MQ=255)
                                          gATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATAcacca                                                                                                       >  6:336315/1‑149 (MQ=255)
                                                cATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                                                  <  5:131851/148‑1 (MQ=255)
                                                   gctttCATCCTATTCGTTGAAAAATTAAATGCCCTGTCGATGCAGATGCCGGCAAACTTCCTGATGCGCCGCGGCCAGGCGCTGGCGCTGGTGTTTTAGGTGGTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                                               <  2:14443/145‑1 (MQ=255)
                                                    ccTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCa                                                                                              >  1:122417/1‑148 (MQ=255)
                                                      ttCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgc                                                                                                                                  >  3:233647/1‑110 (MQ=255)
                                                      ttCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgc                                                                                                                                  <  4:233647/110‑1 (MQ=255)
                                                       tCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCAc                                                                                          >  7:9417/1‑149 (MQ=255)
                                                             gATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                                      >  2:144026/1‑147 (MQ=255)
                                                               ttCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCGAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGAGCTCCATCACCTGCGAgg                                                                                  >  6:93497/1‑149 (MQ=255)
                                                                  ggTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAggtg                                                                                <  3:44864/148‑1 (MQ=255)
                                                                      aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATa                                                                                                            >  7:60073/1‑116 (MQ=255)
                                                                      aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                           >  5:187161/1‑149 (MQ=255)
                                                                      aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                           >  4:111984/1‑149 (MQ=255)
                                                                      aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGcctgg                                                                                                                            <  3:259355/100‑4 (MQ=21)
                                                                      aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGcctgg                                                                                                                            >  4:259355/1‑97 (MQ=21)
                                                                                    cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgcgc                                                                                                                                >  2:342965/1‑82 (MQ=255)
                                                                                    cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgcgc                                                                                                                                <  1:342965/82‑1 (MQ=255)
                                                                                     aGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCCTCACCTGCGAGGTGGTCAGGCCTGAGCGCATCtt                                                            >  1:160688/1‑149 (MQ=255)
                                                                                          aTGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCACCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGt                                                       >  6:268699/1‑149 (MQ=255)
                                                                                           tGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTg                                                      >  4:328822/1‑149 (MQ=255)
                                                                                                 tGCCGGCCAACTTCCTGATGCGCCTGGGCCAGGGGCTGGCGCAGGCGGTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTgcagca                                                 <  8:9417/148‑1 (MQ=255)
                                                                                                           cTTCCTGATGCGCCTCGGCCCGGCGCGGGCGCCGGGGCTTGAGTGGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATc                                      <  6:187161/149‑1 (MQ=255)
                                                                                                                tGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                                 >  4:78792/1‑149 (MQ=255)
                                                                                                                tGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                                 >  4:83587/1‑149 (MQ=255)
                                                                                                                  aTGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                                 <  3:111984/147‑1 (MQ=255)
                                                                                                                  aTGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCg                                <  1:144026/148‑1 (MQ=255)
                                                                                                                    gcgcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                           >  3:105161/1‑103 (MQ=255)
                                                                                                                    gcgcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                           <  4:105161/103‑1 (MQ=255)
                                                                                                                       ccTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCAACTGCGAGGTGGTCCGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                          >  8:47448/1‑149 (MQ=255)
                                                                                                                       ccTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCAAGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                          >  8:42082/1‑149 (MQ=255)
                                                                                                                         tCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCa                                        >  1:143177/1‑133 (MQ=255)
                                                                                                                            gCCAGGCGCTGGCGCAGGCGCTTGAGGTGCATGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGCTGGTCAGGCCTGAGCGCATCATCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTgg                         >  1:31253/1‑145 (MQ=255)
                                                                                                                              cAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGGGTACAGCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGttttt                                           >  5:246950/1‑125 (MQ=255)
                                                                                                                              cAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCa                   <  3:328822/149‑1 (MQ=255)
                                                                                                                                 gcgcTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                          >  7:280725/1‑139 (MQ=38)
                                                                                                                                 gcgcTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                          <  8:280725/139‑1 (MQ=38)
                                                                                                                                   gcTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAg              >  3:13595/1‑149 (MQ=255)
                                                                                                                                          ccggCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGAccc       <  1:183903/147‑1 (MQ=255)
                                                                                                                                               gcTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACg  >  6:178880/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACG  >  NC_002947/2887412‑2887703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑