Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,887,561 G→A 9.7% L370F (CTC→TTC) ‡ PP_2542 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,887,5610GA 9.7% 109.9 / 2.4 41L370F (CTC→TTC) ‡PP_2542GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (23/14);  new base A (2/2);  total (25/16)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

AGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGC  >  NC_002947/2887413‑2887709
                                                                                                                                                    |                                                                                                                                                    
aGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAg                                                                                                                                                      <  5:43984/149‑1 (MQ=255)
aGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAg                                                                                                                                                      <  1:276709/149‑1 (MQ=255)
    aCGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGc                                                                                                                                                  <  5:99252/149‑1 (MQ=255)
            cTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCa                                                                                                                                          <  6:130951/149‑1 (MQ=255)
                                         gATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATAcacca                                                                                                             >  6:336315/1‑149 (MQ=255)
                                               cATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                                                        <  5:131851/148‑1 (MQ=255)
                                                  gctttCATCCTATTCGTTGAAAAATTAAATGCCCTGTCGATGCAGATGCCGGCAAACTTCCTGATGCGCCGCGGCCAGGCGCTGGCGCTGGTGTTTTAGGTGGTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                                                     <  2:14443/145‑1 (MQ=255)
                                                   ccTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCa                                                                                                    >  1:122417/1‑148 (MQ=255)
                                                     ttCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgc                                                                                                                                        <  4:233647/110‑1 (MQ=255)
                                                     ttCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgc                                                                                                                                        >  3:233647/1‑110 (MQ=255)
                                                      tCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCAc                                                                                                >  7:9417/1‑149 (MQ=255)
                                                            gATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                                            >  2:144026/1‑147 (MQ=255)
                                                              ttCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCGAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGAGCTCCATCACCTGCGAgg                                                                                        >  6:93497/1‑149 (MQ=255)
                                                                 ggTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAggtg                                                                                      <  3:44864/148‑1 (MQ=255)
                                                                     aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATa                                                                                                                  >  7:60073/1‑116 (MQ=255)
                                                                     aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                                 >  4:111984/1‑149 (MQ=255)
                                                                     aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                                 >  5:187161/1‑149 (MQ=255)
                                                                     aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGcctgg                                                                                                                                  >  4:259355/1‑97 (MQ=21)
                                                                     aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGcctgg                                                                                                                                  <  3:259355/100‑4 (MQ=21)
                                                                                   cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgcgc                                                                                                                                      <  1:342965/82‑1 (MQ=255)
                                                                                   cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgcgc                                                                                                                                      >  2:342965/1‑82 (MQ=255)
                                                                                    aGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCCTCACCTGCGAGGTGGTCAGGCCTGAGCGCATCtt                                                                  >  1:160688/1‑149 (MQ=255)
                                                                                         aTGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCACCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGt                                                             >  6:268699/1‑149 (MQ=255)
                                                                                          tGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTg                                                            >  4:328822/1‑149 (MQ=255)
                                                                                                tGCCGGCCAACTTCCTGATGCGCCTGGGCCAGGGGCTGGCGCAGGCGGTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTgcagca                                                       <  8:9417/148‑1 (MQ=255)
                                                                                                          cTTCCTGATGCGCCTCGGCCCGGCGCGGGCGCCGGGGCTTGAGTGGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATc                                            <  6:187161/149‑1 (MQ=255)
                                                                                                               tGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                                       >  4:78792/1‑149 (MQ=255)
                                                                                                               tGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                                       >  4:83587/1‑149 (MQ=255)
                                                                                                                 aTGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                                       <  3:111984/147‑1 (MQ=255)
                                                                                                                 aTGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCg                                      <  1:144026/148‑1 (MQ=255)
                                                                                                                   gcgcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                                 >  3:105161/1‑103 (MQ=255)
                                                                                                                   gcgcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                                 <  4:105161/103‑1 (MQ=255)
                                                                                                                      ccTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCAACTGCGAGGTGGTCCGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                                >  8:47448/1‑149 (MQ=255)
                                                                                                                      ccTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCAAGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                                >  8:42082/1‑149 (MQ=255)
                                                                                                                        tCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCa                                              >  1:143177/1‑133 (MQ=255)
                                                                                                                           gCCAGGCGCTGGCGCAGGCGCTTGAGGTGCATGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGCTGGTCAGGCCTGAGCGCATCATCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTgg                               >  1:31253/1‑145 (MQ=255)
                                                                                                                             cAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGGGTACAGCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGttttt                                                 >  5:246950/1‑125 (MQ=255)
                                                                                                                             cAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCa                         <  3:328822/149‑1 (MQ=255)
                                                                                                                                gcgcTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                                >  7:280725/1‑139 (MQ=38)
                                                                                                                                gcgcTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                                <  8:280725/139‑1 (MQ=38)
                                                                                                                                  gcTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAg                    >  3:13595/1‑149 (MQ=255)
                                                                                                                                         ccggCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGAccc             <  1:183903/147‑1 (MQ=255)
                                                                                                                                              gcTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACg        >  6:178880/1‑149 (MQ=255)
                                                                                                                                                    ggtggtCGCGCCAGGGCCCGTCGATGAGCGCCGCATACACGACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGc  <  5:336315/149‑1 (MQ=255)
                                                                                                                                                    ggTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCa                                           <  2:61625/108‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGC  >  NC_002947/2887413‑2887709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑