Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,887,566 T→C 9.6% K368R (AAG→AGG)  PP_2542 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,887,5660TC 9.6% 103.9 / 3.3 42K368R (AAG→AGG) PP_2542GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (22/15);  new base C (2/2);  total (25/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

CTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGCGAA  >  NC_002947/2887425‑2887712
                                                                                                                                             |                                                                                                                                                  
cTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCa                                                                                                                                             <  6:130951/149‑1 (MQ=255)
                             gATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATAcacca                                                                                                                >  6:336315/1‑149 (MQ=255)
                                   cATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                                                           <  5:131851/148‑1 (MQ=255)
                                      gctttCATCCTATTCGTTGAAAAATTAAATGCCCTGTCGATGCAGATGCCGGCAAACTTCCTGATGCGCCGCGGCCAGGCGCTGGCGCTGGTGTTTTAGGTGGTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                                                        <  2:14443/145‑1 (MQ=255)
                                       ccTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCa                                                                                                       >  1:122417/1‑148 (MQ=255)
                                         ttCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgc                                                                                                                                           <  4:233647/110‑1 (MQ=255)
                                         ttCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgc                                                                                                                                           >  3:233647/1‑110 (MQ=255)
                                          tCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCAc                                                                                                   >  7:9417/1‑149 (MQ=255)
                                                gATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                                               >  2:144026/1‑147 (MQ=255)
                                                  ttCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCGAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGAGCTCCATCACCTGCGAgg                                                                                           >  6:93497/1‑149 (MQ=255)
                                                     ggTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAggtg                                                                                         <  3:44864/148‑1 (MQ=255)
                                                         aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATa                                                                                                                     >  7:60073/1‑116 (MQ=255)
                                                         aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                                    >  4:111984/1‑149 (MQ=255)
                                                         aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                                    >  5:187161/1‑149 (MQ=255)
                                                         aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGcctgg                                                                                                                                     >  4:259355/1‑97 (MQ=21)
                                                         aaCAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGcctgg                                                                                                                                     <  3:259355/100‑4 (MQ=21)
                                                                       cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgcgc                                                                                                                                         >  2:342965/1‑82 (MQ=255)
                                                                       cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAgcgc                                                                                                                                         <  1:342965/82‑1 (MQ=255)
                                                                        aGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCCTCACCTGCGAGGTGGTCAGGCCTGAGCGCATCtt                                                                     >  1:160688/1‑149 (MQ=255)
                                                                             aTGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCACCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGt                                                                >  6:268699/1‑149 (MQ=255)
                                                                              tGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTg                                                               >  4:328822/1‑149 (MQ=255)
                                                                                    tGCCGGCCAACTTCCTGATGCGCCTGGGCCAGGGGCTGGCGCAGGCGGTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTgcagca                                                          <  8:9417/148‑1 (MQ=255)
                                                                                              cTTCCTGATGCGCCTCGGCCCGGCGCGGGCGCCGGGGCTTGAGTGGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATc                                               <  6:187161/149‑1 (MQ=255)
                                                                                                   tGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                                          >  4:78792/1‑149 (MQ=255)
                                                                                                   tGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                                          >  4:83587/1‑149 (MQ=255)
                                                                                                     aTGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTc                                          <  3:111984/147‑1 (MQ=255)
                                                                                                     aTGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCg                                         <  1:144026/148‑1 (MQ=255)
                                                                                                       gcgcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                                    >  3:105161/1‑103 (MQ=255)
                                                                                                       gcgcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAg                                                                                    <  4:105161/103‑1 (MQ=255)
                                                                                                          ccTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCAACTGCGAGGTGGTCCGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                                   >  8:47448/1‑149 (MQ=255)
                                                                                                          ccTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCAAGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                                   >  8:42082/1‑149 (MQ=255)
                                                                                                            tCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCa                                                 >  1:143177/1‑133 (MQ=255)
                                                                                                               gCCAGGCGCTGGCGCAGGCGCTTGAGGTGCATGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGCTGGTCAGGCCTGAGCGCATCATCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTgg                                  >  1:31253/1‑145 (MQ=255)
                                                                                                                 cAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGGGTACAGCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGttttt                                                    >  5:246950/1‑125 (MQ=255)
                                                                                                                 cAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCa                            <  3:328822/149‑1 (MQ=255)
                                                                                                                    gcgcTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                                   >  7:280725/1‑139 (MQ=38)
                                                                                                                    gcgcTGGCGCAAGCACCTCAAGCGCCTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                                   <  8:280725/139‑1 (MQ=38)
                                                                                                                      gcTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAg                       >  3:13595/1‑149 (MQ=255)
                                                                                                                             ccggCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGAccc                <  1:183903/147‑1 (MQ=255)
                                                                                                                                  gcTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACg           >  6:178880/1‑149 (MQ=255)
                                                                                                                                        ggtggtCGCGCCAGGGCCCGTCGATGAGCGCCGCATACACGACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGc     <  5:336315/149‑1 (MQ=255)
                                                                                                                                        ggTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCa                                              <  2:61625/108‑1 (MQ=255)
                                                                                                                                           gCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCACCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGCGaa  <  5:268699/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
CTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCGCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGCGAA  >  NC_002947/2887425‑2887712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑