Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,890,383 G→A 14.9% R464H (CGC→CAC)  gabP‑II → gamma‑aminobutyrate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,890,3830GA14.9% 110.4 / 9.9 47R464H (CGC→CAC) gabP‑IIgamma‑aminobutyrate permease
Reads supporting (aligned to +/- strand):  ref base G (22/18);  new base A (4/3);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

GTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTGAACCGAGCAGGGCTG  >  NC_002947/2890243‑2890530
                                                                                                                                             |                                                                                                                                                   
gTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCActc                                                                                                                                                  >  1:193929/1‑144 (MQ=255)
gTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCActc                                                                                                                                                  <  2:193929/144‑1 (MQ=255)
     tGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGctct                                                                                                                                                 <  6:113333/140‑1 (MQ=255)
     tGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGctct                                                                                                                                                 >  5:113333/1‑140 (MQ=255)
         ctacctGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTagcaagc                                                                                                                                       <  8:68800/146‑1 (MQ=255)
         ctacctGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTagcaagc                                                                                                                                       >  7:68800/1‑146 (MQ=255)
         ctacctGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAagcag                                                                                                                                     <  1:134597/148‑1 (MQ=255)
         ctacctGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAagcag                                                                                                                                     >  2:134597/1‑148 (MQ=255)
                             tCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCctct                                                                                                                >  4:136998/1‑149 (MQ=255)
                                  gCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGaaa                                                                                                           >  7:12267/1‑149 (MQ=255)
                                      cccTGGGCACCATGGCCTTCATGCCTGACTACCAGATGCTGGTCATTTGCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAAcgg                                                                                                        <  8:146834/148‑1 (MQ=255)
                                       ccTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTaa                                                                                                                               >  2:44092/1‑124 (MQ=255)
                                       ccTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTaa                                                                                                                               <  1:44092/124‑1 (MQ=255)
                                       ccTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAAcggcc                                                                                                      >  6:353363/1‑149 (MQ=255)
                                                                gACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGGAGCCGGcc                                                                                 <  6:172203/145‑1 (MQ=255)
                                                                gACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGGAGCCGGcc                                                                                 >  5:172203/1‑145 (MQ=255)
                                                                     ccAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTg                                                                                                               <  8:206572/110‑1 (MQ=255)
                                                                     ccAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTg                                                                                                               >  7:206572/1‑110 (MQ=255)
                                                                     ccAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTcgc                                                                          <  5:353363/147‑1 (MQ=255)
                                                                                tCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTg                                                             <  8:12267/149‑1 (MQ=255)
                                                                                 cATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCACTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTgg                                                            >  5:159257/1‑149 (MQ=255)
                                                                                   tttCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCt                                                              <  4:250604/145‑1 (MQ=255)
                                                                                   tttCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCt                                                              >  3:250604/1‑145 (MQ=255)
                                                                                       cACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCCGCTCCCTTGGGGACCCGGCCGTTTCGCGGTGTTGGGCCTGGGCtttt                                                      >  2:98856/1‑149 (MQ=255)
                                                                                        aCCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCttttt                                                     <  3:136998/149‑1 (MQ=255)
                                                                                               tCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCACTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCgg                                               <  6:159257/148‑1 (MQ=255)
                                                                                                 gCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCTAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCggg                                              >  1:63450/1‑148 (MQ=255)
                                                                                                    gcggcgGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCctct                                                                                                                <  2:35232/78‑1 (MQ=255)
                                                                                                    gcggcgGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCAtgt                                         >  2:82037/1‑149 (MQ=255)
                                                                                                         ggtggtggtGGCTATGGGTGTGGTGCACC‑AGATCGGGTCTGGTAGCATGCAGCACTAAGCGTCACTCGCGTCTTGAAACGGCCGGCTCCCTTGGGGACCCGGCGGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCgg                                               <  6:51483/138‑1 (MQ=255)
                                                                                                                  ggCGATGGGTGTGGTTCACC‑AGATCCGGTCTGGTAGCAAGCAGCTCTAAGCGGCACTCGCCTCTGGAATCGGCCGGCTCCCTTGGGGACCCGGGCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTc                           <  3:5046/149‑1 (MQ=255)
                                                                                                                  ggCGATGGGTGTGGTGCACC‑AGATCCACTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGtt                            >  4:77842/1‑148 (MQ=255)
                                                                                                                   gCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTc                           <  2:63450/148‑1 (MQ=255)
                                                                                                                     gATGGGTGTGGTGCACC‑AGATCCACTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTCATc                        >  4:120224/1‑149 (MQ=255)
                                                                                                                              ttgctaCC‑AGAGCGGATCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGGCCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTGaa               >  8:233616/6‑149 (MQ=255)
                                                                                                                               gtgCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTGAAc              >  7:268457/1‑149 (MQ=255)
                                                                                                                               gtgCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCCGCTCCCGTGGGGGCCCCGCCGGTTCGGGTTGTTGGGGCGTGGGTTTTTGTGGGGGCATGTGGTAACGCAGGTTCATACACCGTgtaa              >  5:47237/1‑146 (MQ=255)
                                                                                                                                 gCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTgg                              <  7:55811/131‑1 (MQ=255)
                                                                                                                                 gCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTgg                              >  8:55811/1‑131 (MQ=255)
                                                                                                                                  cACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGACACGCTGGTTCATCCACGGTGAACCGa           <  1:82037/149‑1 (MQ=255)
                                                                                                                                  cACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTGAAcc             >  5:148052/1‑147 (MQ=255)
                                                                                                                                   aCC‑AGATCCACTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTGAACCGAg          <  3:77842/149‑1 (MQ=255)
                                                                                                                                    cc‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCCGCTACCGTGGGGACCCGGCCGTGTAGCGTTGTTGGGCCGGGGGTTTTTATGCGGGCAAGTGGAAACGCTGGTTCATCCCACGTGAACCGAg          >  8:292463/1‑148 (MQ=255)
                                                                                                                                        gATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCAtgt                                         <  3:130077/114‑1 (MQ=255)
                                                                                                                                        gATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCAtgt                                         >  4:130077/1‑114 (MQ=255)
                                                                                                                                         aTCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTGAACCGAGCAggg     >  2:40535/1‑149 (MQ=255)
                                                                                                                                            cGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTGAACCGAGCAGGGCTg  >  2:274491/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
GTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACC‑AGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGGACCCGGCCGTTTCGCGTTGTTGGGCCTGGGCTTTTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTGAACCGAGCAGGGCTG  >  NC_002947/2890243‑2890530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑