| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,902,856 | G→A | 18.7% | intergenic (+30/+39) | quiC → / ← PP_2555 | 3‑dehydroshikimate dehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,902,856 | 0 | G | A | 18.7% | 65.9 / 8.3 | 32 | intergenic (+30/+39) | quiC/PP_2555 | 3‑dehydroshikimate dehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (12/14); new base A (2/4); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01 | |||||||||||
CGAGATCATCCAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGATGCTGCGGTTCCTGCAGACTGACCAGCCTCAACCCCGCCATGTCCAGCGCA > NC_002947/2902712‑2902997 | cGAGATCATCCAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTggg < 4:251632/147‑1 (MQ=255)cGAGATCATCCAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTggg < 4:159012/147‑1 (MQ=255) gagaTCATCCAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGTCTGTCTTGTGGGAGTGGGGt < 6:330008/148‑1 (MQ=255) gaTCATCCAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGTGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGt < 1:4241/146‑1 (MQ=255) gaTCATCCAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGt > 2:4241/1‑146 (MQ=255) gaTCATCCAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGAAGCAGGGttca > 4:259929/1‑146 (MQ=255) tcatcCAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAAcc < 2:57497/149‑1 (MQ=255) tcCAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTg > 6:360378/1‑149 (MQ=255) ccAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGc > 1:106872/1‑149 (MQ=255) cAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCt < 4:208239/149‑1 (MQ=255) cAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGAAGCAGGGTTCACCCCACTCCcac < 7:250308/149‑1 (MQ=255) aaGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCcaca < 6:252904/149‑1 (MQ=255) aGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAg < 5:360378/149‑1 (MQ=255) aGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGAAGCAGGGTTCACCCCACTCCcacaa < 3:259929/149‑2 (MQ=255) gCGGGTTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGTGTGAACCCTGCTTCCACAGGa < 6:355142/149‑1 (MQ=255) ggTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGattat > 4:123158/1‑149 (MQ=255) tACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTAGGGTGAACCCTGCTTCCACAGGAttatta < 7:302587/149‑1 (MQ=255) ttACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGa < 2:106872/149‑1 (MQ=255) ttGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGccc > 7:292927/1‑130 (MQ=255) ttGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGccc < 8:292927/130‑1 (MQ=255) ttGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGc > 5:347623/1‑148 (MQ=255) ccATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGAAGCAGGGTTCACCCCACTCCcacaa < 5:250336/98‑2 (MQ=25) ccATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGAAGCAGGGTTCACCCCACTCCcacaa > 6:250336/1‑97 (MQ=25) gggCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGAtgc < 3:123158/149‑1 (MQ=255) gcAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGATGCTGCGGTTcc > 8:31044/1‑149 (MQ=255) gcAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGATGCTGCGGTTcc > 2:16891/1‑149 (MQ=255) gCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGATGCTGCGGTTCCTGCa < 6:347623/149‑1 (MQ=255) ccGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGATGCTGCGGTTCCTg > 6:112054/1‑146 (MQ=255) cGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGATGCTGCGGTTCCTGCAGa > 8:2365/1‑149 (MQ=255) tAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGATGCTGCGGTTCCTGCAGACTGAcc < 7:31044/147‑1 (MQ=255) gCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCAcc > 2:263170/1‑102 (MQ=255) gCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCAcc < 1:263170/102‑1 (MQ=255) cGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGATGCTGCGGTTCCTGCAGACTGACCAGCCTCAACCCCGc > 2:234398/1‑148 (MQ=255) tgGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGATGCTGCGGTTCCTGCAGACTGACCAGCCTCAACCCCGCCATGTCCAGCGCa < 1:16891/149‑1 (MQ=255) | CGAGATCATCCAGCGCAAGGCGGGGTACGCTGGTTACGGCGCTGCCAACGTTGCGGTGCGCCTGGCAGCCATGGCCAAGGCCCGTAGCGGGGCGGCGCGCAAGCCGGTGCTGTAGTCGCCTGCACCGGCCTGTCTTGTGGGAGTGGGGTGAACCCTGCTTCCACAGGATTATTACGGCCCGATTCAGTGACGCTCAGCCACCATCAACAACGACTGCGGCACCGCGCTCTGCGGATGCTGCGGTTCCTGCAGACTGACCAGCCTCAACCCCGCCATGTCCAGCGCA > NC_002947/2902712‑2902997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |